Integrated Transcriptome and Metabolomics Analysis Reveals That Probiotics and Tea Polyphenols Synergetically Regulate Lipid Metabolism in Laying Hens
Tea polyphenols (TP) and probiotics (PB) have been recognized for their ability to improve lipid metabolism and regulate immune function. However, their specific impact on lipid metabolism in laying hens has not been thoroughly elucidated. Therefore, this study sought to examine the effect of TP and...
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2024-11-01
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| author | Ming Qin Cai Ma Zengguang Wang Mingzhi Liang Yufen Sha Jiewei Liu Shunjin Ge Longzong Guo Ruili Li |
| author_facet | Ming Qin Cai Ma Zengguang Wang Mingzhi Liang Yufen Sha Jiewei Liu Shunjin Ge Longzong Guo Ruili Li |
| author_sort | Ming Qin |
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| description | Tea polyphenols (TP) and probiotics (PB) have been recognized for their ability to improve lipid metabolism and regulate immune function. However, their specific impact on lipid metabolism in laying hens has not been thoroughly elucidated. Therefore, this study sought to examine the effect of TP and <i>Bacillus subtilis</i> on lipid metabolism in laying hens through transcriptome and metabolome analyses. Two hundred Hy-line Brown layers were randomly allocated into four groups with supplemental dietary TP and PB alone and their combination for 8 weeks. Each treatment had 10 replicates of five birds. Supplementation with a TP and PB combination (TP-PB) increased redness (a*) (<i>p</i> < 0.05) compared to the control basal diet (CT). Dietary TP-PB decreased egg yolk and serum total cholesterol (TC) concentrations (<i>p</i> < 0.05) without affecting the content of total bile acid (TBA). The combined use of TP and PB significantly improved hepatic fatty acid synthetase (FAS) activity (<i>p</i> < 0.05) and reduced liver fat particles. Dietary TP-PB primarily influenced the transcript levels of genes involved in fat metabolic pathways. In particular, TP-PB supplementation reduced lipid storage by activating the Notch signaling pathway. Furthermore, the addition of TP-PB in the diet modulated the abundance of metabolic biomarkers associated with bile secretion and valine, leucine, and isoleucine degradation. An interaction network of mRNAs and metabolites was constructed associated with lipid metabolism, such as deoxycholic acid, TAG (14:3–14:3–20:5), PDK4, and HES4. Overall, these findings emphasized the potential health advantages of the TP and PB combination as a possible functional feed supplement in livestock nutrition. |
| format | Article |
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| institution | Kabale University |
| issn | 2077-0472 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Agriculture |
| spelling | doaj-art-b2f901d36a8d4c9aafe41be9463b41a32024-11-26T17:43:56ZengMDPI AGAgriculture2077-04722024-11-011411207210.3390/agriculture14112072Integrated Transcriptome and Metabolomics Analysis Reveals That Probiotics and Tea Polyphenols Synergetically Regulate Lipid Metabolism in Laying HensMing Qin0Cai Ma1Zengguang Wang2Mingzhi Liang3Yufen Sha4Jiewei Liu5Shunjin Ge6Longzong Guo7Ruili Li8Institute of Animal Science and Veterinary Medicine, Yantai Academy of Agricultural Sciences, Yantai 265500, ChinaDepartment of Medical Genetics and Cell Biology, Binzhou Medical University, Yantai 265500, ChinaInstitute of Animal Science and Veterinary Medicine, Yantai Academy of Agricultural Sciences, Yantai 265500, ChinaInstitute of Animal Science and Veterinary Medicine, Yantai Academy of Agricultural Sciences, Yantai 265500, ChinaInstitute of Animal Science and Veterinary Medicine, Yantai Academy of Agricultural Sciences, Yantai 265500, ChinaShandong Xiantan Co., Ltd., Yantai 265500, ChinaShandong Xiantan Co., Ltd., Yantai 265500, ChinaShandong Yisheng Livestock and Poultry Breeding Co., Ltd., Yantai 265500, ChinaInstitute of Animal Science and Veterinary Medicine, Yantai Academy of Agricultural Sciences, Yantai 265500, ChinaTea polyphenols (TP) and probiotics (PB) have been recognized for their ability to improve lipid metabolism and regulate immune function. However, their specific impact on lipid metabolism in laying hens has not been thoroughly elucidated. Therefore, this study sought to examine the effect of TP and <i>Bacillus subtilis</i> on lipid metabolism in laying hens through transcriptome and metabolome analyses. Two hundred Hy-line Brown layers were randomly allocated into four groups with supplemental dietary TP and PB alone and their combination for 8 weeks. Each treatment had 10 replicates of five birds. Supplementation with a TP and PB combination (TP-PB) increased redness (a*) (<i>p</i> < 0.05) compared to the control basal diet (CT). Dietary TP-PB decreased egg yolk and serum total cholesterol (TC) concentrations (<i>p</i> < 0.05) without affecting the content of total bile acid (TBA). The combined use of TP and PB significantly improved hepatic fatty acid synthetase (FAS) activity (<i>p</i> < 0.05) and reduced liver fat particles. Dietary TP-PB primarily influenced the transcript levels of genes involved in fat metabolic pathways. In particular, TP-PB supplementation reduced lipid storage by activating the Notch signaling pathway. Furthermore, the addition of TP-PB in the diet modulated the abundance of metabolic biomarkers associated with bile secretion and valine, leucine, and isoleucine degradation. An interaction network of mRNAs and metabolites was constructed associated with lipid metabolism, such as deoxycholic acid, TAG (14:3–14:3–20:5), PDK4, and HES4. Overall, these findings emphasized the potential health advantages of the TP and PB combination as a possible functional feed supplement in livestock nutrition.https://www.mdpi.com/2077-0472/14/11/2072tea polyphenolsprobioticslipid metabolismtranscriptomemetabolomemRNA-metabolite network |
| spellingShingle | Ming Qin Cai Ma Zengguang Wang Mingzhi Liang Yufen Sha Jiewei Liu Shunjin Ge Longzong Guo Ruili Li Integrated Transcriptome and Metabolomics Analysis Reveals That Probiotics and Tea Polyphenols Synergetically Regulate Lipid Metabolism in Laying Hens Agriculture tea polyphenols probiotics lipid metabolism transcriptome metabolome mRNA-metabolite network |
| title | Integrated Transcriptome and Metabolomics Analysis Reveals That Probiotics and Tea Polyphenols Synergetically Regulate Lipid Metabolism in Laying Hens |
| title_full | Integrated Transcriptome and Metabolomics Analysis Reveals That Probiotics and Tea Polyphenols Synergetically Regulate Lipid Metabolism in Laying Hens |
| title_fullStr | Integrated Transcriptome and Metabolomics Analysis Reveals That Probiotics and Tea Polyphenols Synergetically Regulate Lipid Metabolism in Laying Hens |
| title_full_unstemmed | Integrated Transcriptome and Metabolomics Analysis Reveals That Probiotics and Tea Polyphenols Synergetically Regulate Lipid Metabolism in Laying Hens |
| title_short | Integrated Transcriptome and Metabolomics Analysis Reveals That Probiotics and Tea Polyphenols Synergetically Regulate Lipid Metabolism in Laying Hens |
| title_sort | integrated transcriptome and metabolomics analysis reveals that probiotics and tea polyphenols synergetically regulate lipid metabolism in laying hens |
| topic | tea polyphenols probiotics lipid metabolism transcriptome metabolome mRNA-metabolite network |
| url | https://www.mdpi.com/2077-0472/14/11/2072 |
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