Protocol for evaluating the activity of R2 retrotransposons in mammalian cells
Summary: R2 retrotransposons can be harnessed to insert genes at targeted sites by all-RNA delivery, presenting a new technology for next-generation biotherapeutics. Here, we report a protocol for evaluating the gene integration activity of R2 retrotransposons in mammalian cells. We describe the con...
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Main Authors: | Yangcan Chen, Shengqiu Luo, Yanping Hu, Qi Zhou, Wei Li |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2025-03-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007032 |
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