Protocol for evaluating the activity of R2 retrotransposons in mammalian cells

Summary: R2 retrotransposons can be harnessed to insert genes at targeted sites by all-RNA delivery, presenting a new technology for next-generation biotherapeutics. Here, we report a protocol for evaluating the gene integration activity of R2 retrotransposons in mammalian cells. We describe the con...

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Bibliographic Details
Main Authors: Yangcan Chen, Shengqiu Luo, Yanping Hu, Qi Zhou, Wei Li
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724007032
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