Stiffness and surface topology of silicone implants competitively mediate inflammatory responses of macrophages and foreign body response

Adverse inflammatory responses, dominated by macrophages, that are induced by physical cues of silicone implants can heavily damage the life quality of patients via causing fibrosis and device failure. As stiffness and surface topology affect macrophages at the same time, the competition or partners...

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Main Authors: Sicen He, Qingrong Zhang, Jiezhi Jia, Wei Xia, Shengnan Chen, Fanyi Min, Yanlin Song, Yunlong Yu, Jiangfeng Li, Zheng Li, Gaoxing Luo
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Materials Today Bio
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Online Access:http://www.sciencedirect.com/science/article/pii/S259000642400365X
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author Sicen He
Qingrong Zhang
Jiezhi Jia
Wei Xia
Shengnan Chen
Fanyi Min
Yanlin Song
Yunlong Yu
Jiangfeng Li
Zheng Li
Gaoxing Luo
author_facet Sicen He
Qingrong Zhang
Jiezhi Jia
Wei Xia
Shengnan Chen
Fanyi Min
Yanlin Song
Yunlong Yu
Jiangfeng Li
Zheng Li
Gaoxing Luo
author_sort Sicen He
collection DOAJ
description Adverse inflammatory responses, dominated by macrophages, that are induced by physical cues of silicone implants can heavily damage the life quality of patients via causing fibrosis and device failure. As stiffness and surface topology affect macrophages at the same time, the competition or partnership among physical cues against the regulation of macrophages is still ambiguous. Herein, a series of PDMS implants with different stiffness at ∼ MPa and surface topology at tens of micrometers were fabricated to investigate the relationship, the regulation rule, and the underlying mechanism of the two physical cues against the inflammatory responses of M1 macrophages. There is a competitive rule: surface topology could suppress the inflammatory responses of M1 macrophages in the soft group but did not have the same effect in the stiff group. Without surface topology, lower stiffness unexpectedly evoked stronger inflammatory responses of M1 macrophages. Implanting experiments also proved that the competitive state against mediating in vivo immune responses and the unexpected inflammatory responses. The reason is that stiffness could strongly up-regulate focal adhesion and activate the MAPK/NF-κB signaling axis to evoke inflammatory responses, which could shield the effect of surface topology. Therefore, for patient healthcare, it is crucial to prioritize stiffness while not surface topology at MPa levels to minimize adverse reactions.
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series Materials Today Bio
spelling doaj-art-1d405bf0035c4de987e63bde37a0e66d2024-12-14T06:32:13ZengElsevierMaterials Today Bio2590-00642024-12-0129101304Stiffness and surface topology of silicone implants competitively mediate inflammatory responses of macrophages and foreign body responseSicen He0Qingrong Zhang1Jiezhi Jia2Wei Xia3Shengnan Chen4Fanyi Min5Yanlin Song6Yunlong Yu7Jiangfeng Li8Zheng Li9Gaoxing Luo10Institute of Burn Research, Southwest Hospital & State Key Lab of Trauma and Chemical Poisoning, Third Military Medical University (Army Medical University), Chongqing, 400038, PR ChinaInstitute of Burn Research, Southwest Hospital & State Key Lab of Trauma and Chemical Poisoning, Third Military Medical University (Army Medical University), Chongqing, 400038, PR ChinaInstitute of Burn Research, Southwest Hospital & State Key Lab of Trauma and Chemical Poisoning, Third Military Medical University (Army Medical University), Chongqing, 400038, PR ChinaInstitute of Burn Research, Southwest Hospital & State Key Lab of Trauma and Chemical Poisoning, Third Military Medical University (Army Medical University), Chongqing, 400038, PR ChinaKey Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences (ICCAS), Beijing Engineering Research Center of Nanomaterials for Green Printing Technology, Beijing National Laboratory for Molecular Sciences (BNLMS), Beijing, 100190, PR China; School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, 100049, PR ChinaKey Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences (ICCAS), Beijing Engineering Research Center of Nanomaterials for Green Printing Technology, Beijing National Laboratory for Molecular Sciences (BNLMS), Beijing, 100190, PR China; School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, 100049, PR ChinaKey Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences (ICCAS), Beijing Engineering Research Center of Nanomaterials for Green Printing Technology, Beijing National Laboratory for Molecular Sciences (BNLMS), Beijing, 100190, PR China; School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, 100049, PR ChinaInstitute of Burn Research, Southwest Hospital & State Key Lab of Trauma and Chemical Poisoning, Third Military Medical University (Army Medical University), Chongqing, 400038, PR China; Corresponding author.Institute of Burn Research, Southwest Hospital & State Key Lab of Trauma and Chemical Poisoning, Third Military Medical University (Army Medical University), Chongqing, 400038, PR China; Corresponding author.Institute of Burn Research, Southwest Hospital & State Key Lab of Trauma and Chemical Poisoning, Third Military Medical University (Army Medical University), Chongqing, 400038, PR China; Corresponding author.Institute of Burn Research, Southwest Hospital & State Key Lab of Trauma and Chemical Poisoning, Third Military Medical University (Army Medical University), Chongqing, 400038, PR China; Corresponding author.Adverse inflammatory responses, dominated by macrophages, that are induced by physical cues of silicone implants can heavily damage the life quality of patients via causing fibrosis and device failure. As stiffness and surface topology affect macrophages at the same time, the competition or partnership among physical cues against the regulation of macrophages is still ambiguous. Herein, a series of PDMS implants with different stiffness at ∼ MPa and surface topology at tens of micrometers were fabricated to investigate the relationship, the regulation rule, and the underlying mechanism of the two physical cues against the inflammatory responses of M1 macrophages. There is a competitive rule: surface topology could suppress the inflammatory responses of M1 macrophages in the soft group but did not have the same effect in the stiff group. Without surface topology, lower stiffness unexpectedly evoked stronger inflammatory responses of M1 macrophages. Implanting experiments also proved that the competitive state against mediating in vivo immune responses and the unexpected inflammatory responses. The reason is that stiffness could strongly up-regulate focal adhesion and activate the MAPK/NF-κB signaling axis to evoke inflammatory responses, which could shield the effect of surface topology. Therefore, for patient healthcare, it is crucial to prioritize stiffness while not surface topology at MPa levels to minimize adverse reactions.http://www.sciencedirect.com/science/article/pii/S259000642400365XMacrophagesInflammatory responseStiffnessSurface topologyImplants
spellingShingle Sicen He
Qingrong Zhang
Jiezhi Jia
Wei Xia
Shengnan Chen
Fanyi Min
Yanlin Song
Yunlong Yu
Jiangfeng Li
Zheng Li
Gaoxing Luo
Stiffness and surface topology of silicone implants competitively mediate inflammatory responses of macrophages and foreign body response
Materials Today Bio
Macrophages
Inflammatory response
Stiffness
Surface topology
Implants
title Stiffness and surface topology of silicone implants competitively mediate inflammatory responses of macrophages and foreign body response
title_full Stiffness and surface topology of silicone implants competitively mediate inflammatory responses of macrophages and foreign body response
title_fullStr Stiffness and surface topology of silicone implants competitively mediate inflammatory responses of macrophages and foreign body response
title_full_unstemmed Stiffness and surface topology of silicone implants competitively mediate inflammatory responses of macrophages and foreign body response
title_short Stiffness and surface topology of silicone implants competitively mediate inflammatory responses of macrophages and foreign body response
title_sort stiffness and surface topology of silicone implants competitively mediate inflammatory responses of macrophages and foreign body response
topic Macrophages
Inflammatory response
Stiffness
Surface topology
Implants
url http://www.sciencedirect.com/science/article/pii/S259000642400365X
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