Probing spatiotemporally organized GPCR signaling using genetically encoded molecular tools
Abstract G-protein-coupled receptors (GPCRs) control various downstream signaling pathways, with multiple effectors whose interactions are subject to sophisticated regulation to achieve signaling specificity. Spatiotemporal organization of GPCR signaling is essential for efficient control of multifa...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2025-07-01
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| Series: | Experimental and Molecular Medicine |
| Online Access: | https://doi.org/10.1038/s12276-025-01485-2 |
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| _version_ | 1849344838015647744 |
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| author | Yonghoon Kwon Sohum Mehta Jin Zhang |
| author_facet | Yonghoon Kwon Sohum Mehta Jin Zhang |
| author_sort | Yonghoon Kwon |
| collection | DOAJ |
| description | Abstract G-protein-coupled receptors (GPCRs) control various downstream signaling pathways, with multiple effectors whose interactions are subject to sophisticated regulation to achieve signaling specificity. Spatiotemporal organization of GPCR signaling is essential for efficient control of multifaceted signaling pathways. To study how this spatiotemporal signaling is structured and affects cellular functionality, various genetically encoded molecular tools that can detect and perturb the target biochemical activities at a subcellular level have been developed. In this Review, we introduce various types of fluorescent protein-based biosensors and molecular tools that allow us to directly elucidate the spatiotemporal mechanisms of GPCR signaling regulation at a subcellular level. Finally, we highlight several applications of these molecular tools to study the spatiotemporal organization of GPCR in living cells to obtain a comprehensive understanding of the signaling architecture. |
| format | Article |
| id | doaj-art-0e2fa86d41f44c19944e2ac0d06e7a61 |
| institution | Kabale University |
| issn | 2092-6413 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Experimental and Molecular Medicine |
| spelling | doaj-art-0e2fa86d41f44c19944e2ac0d06e7a612025-08-20T03:42:34ZengNature Publishing GroupExperimental and Molecular Medicine2092-64132025-07-015771432144210.1038/s12276-025-01485-2Probing spatiotemporally organized GPCR signaling using genetically encoded molecular toolsYonghoon Kwon0Sohum Mehta1Jin Zhang2Department of Pharmacology, University of California San DiegoDepartment of Pharmacology, University of California San DiegoDepartment of Pharmacology, University of California San DiegoAbstract G-protein-coupled receptors (GPCRs) control various downstream signaling pathways, with multiple effectors whose interactions are subject to sophisticated regulation to achieve signaling specificity. Spatiotemporal organization of GPCR signaling is essential for efficient control of multifaceted signaling pathways. To study how this spatiotemporal signaling is structured and affects cellular functionality, various genetically encoded molecular tools that can detect and perturb the target biochemical activities at a subcellular level have been developed. In this Review, we introduce various types of fluorescent protein-based biosensors and molecular tools that allow us to directly elucidate the spatiotemporal mechanisms of GPCR signaling regulation at a subcellular level. Finally, we highlight several applications of these molecular tools to study the spatiotemporal organization of GPCR in living cells to obtain a comprehensive understanding of the signaling architecture.https://doi.org/10.1038/s12276-025-01485-2 |
| spellingShingle | Yonghoon Kwon Sohum Mehta Jin Zhang Probing spatiotemporally organized GPCR signaling using genetically encoded molecular tools Experimental and Molecular Medicine |
| title | Probing spatiotemporally organized GPCR signaling using genetically encoded molecular tools |
| title_full | Probing spatiotemporally organized GPCR signaling using genetically encoded molecular tools |
| title_fullStr | Probing spatiotemporally organized GPCR signaling using genetically encoded molecular tools |
| title_full_unstemmed | Probing spatiotemporally organized GPCR signaling using genetically encoded molecular tools |
| title_short | Probing spatiotemporally organized GPCR signaling using genetically encoded molecular tools |
| title_sort | probing spatiotemporally organized gpcr signaling using genetically encoded molecular tools |
| url | https://doi.org/10.1038/s12276-025-01485-2 |
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