Ginsenoside Rh2 inhibits breast cancer cell growth via<?A3B2 ACK?>ERβ-TNFα pathway
Ginsenoside Rh2 is one of rare panaxidiols extracted from Panax ginseng and a potential estrogen receptor ligand that exhibits moderate estrogenic activity. However, the effect of Rh2 on growth inhibition and its underlying molecular mechanism in human breast cells are not fully understood. In this...
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China Science Publishing & Media Ltd.
2022-05-01
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| Series: | Acta Biochimica et Biophysica Sinica |
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| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2022039 |
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| author | Peng Kunjian Luo Tiao Li Jijia Huang Jingjia Dong Zizeng Liu Jia Pi Chaoqiong Zou Zizeng Gu Qin Liu Ousheng Zhang Jian-Ting Luo Zhi-Yong |
| author_facet | Peng Kunjian Luo Tiao Li Jijia Huang Jingjia Dong Zizeng Liu Jia Pi Chaoqiong Zou Zizeng Gu Qin Liu Ousheng Zhang Jian-Ting Luo Zhi-Yong |
| author_sort | Peng Kunjian |
| collection | DOAJ |
| description | Ginsenoside Rh2 is one of rare panaxidiols extracted from Panax ginseng and a potential estrogen receptor ligand that exhibits moderate estrogenic activity. However, the effect of Rh2 on growth inhibition and its underlying molecular mechanism in human breast cells are not fully understood. In this study, we tested cell viability by MTT and colony formation assays. Cell growth and cell cycle were determined to investigate the effect of ginsenoside Rh2 by flow cytometry. The expressions of estrogen receptors (ERs), TNFα, and apoptosis-related proteins were detected by qPCR and western blot analysis. The mechanisms of ERα and ERβ action were determined using transfection and inhibitors. Antitumor effect of ginsenoside Rh2 against MCF-7 cells was investigated in xenograft mice. Our results showed that ginsenoside Rh2 induced apoptosis and G1/S phase arrest in MCF-7 cells. Treatment of cells with ginsenoside Rh2 down-regulated protein levels of ERα, and up-regulated mRNA and protein levels of ERβ and TNFα. We also found that ginsenoside Rh2-induced TNFα over-expression is through up-regulation of ERβ initiated by ginsenoside Rh2. Furthermore, ginsenoside Rh2 induced MCF-7 cell apoptosis via estrogen receptor β-TNFα pathway in vivo. These results demonstrate that ginsenoside Rh2 promotes TNFα-induced apoptosis and G1/S phase arrest via regulation of ERβ. |
| format | Article |
| id | doaj-art-feb6cdcb11ea49d89c74f23d1b34b97d |
| institution | Kabale University |
| issn | 1672-9145 |
| language | English |
| publishDate | 2022-05-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj-art-feb6cdcb11ea49d89c74f23d1b34b97d2025-08-20T03:53:46ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452022-05-015464765610.3724/abbs.202203920d259ccGinsenoside Rh2 inhibits breast cancer cell growth via<?A3B2 ACK?>ERβ-TNFα pathwayPeng Kunjian0Luo Tiao1Li Jijia2Huang Jingjia3Dong Zizeng4Liu Jia5Pi Chaoqiong6Zou Zizeng7Gu Qin8Liu Ousheng9Zhang Jian-Ting10Luo Zhi-Yong11["Department of Biochemistry and Molecular Biology, Hunan Province Key Laboratory of Basic and Applied Hematology & Hunan Key Laboratory of Animal Models for Human Diseases, School of Life Sciences, Central South University, Changsha 410008, China"]["Hunan Key Laboratory of Oral Health Research & Xiangya Stomatological Hospital & Xiangya School of Stomatology, Central South University, Changsha 410008, China"]["Center of Stomatology, Xiangya Hospital, Central South University, Changsha 410008, China."]["Department of Biochemistry and Molecular Biology, Hunan Province Key Laboratory of Basic and Applied Hematology & Hunan Key Laboratory of Animal Models for Human Diseases, School of Life Sciences, Central South University, Changsha 410008, China"]["Department of Cell and Cancer Biology, University of Toledo College of Medicine and Life Sciences, Toledo, OH 43614, USA"]["Department of Biochemistry and Molecular Biology, Hunan Province Key Laboratory of Basic and Applied Hematology & Hunan Key Laboratory of Animal Models for Human Diseases, School of Life Sciences, Central South University, Changsha 410008, China"]["Department of Biochemistry and Molecular Biology, Hunan Province Key Laboratory of Basic and Applied Hematology & Hunan Key Laboratory of Animal Models for Human Diseases, School of Life Sciences, Central South University, Changsha 410008, China"]["Department of Biochemistry and Molecular Biology, Hunan Province Key Laboratory of Basic and Applied Hematology & Hunan Key Laboratory of Animal Models for Human Diseases, School of Life Sciences, Central South University, Changsha 410008, China"]["Department of Biochemistry and Molecular Biology, Hunan Province Key Laboratory of Basic and Applied Hematology & Hunan Key Laboratory of Animal Models for Human Diseases, School of Life Sciences, Central South University, Changsha 410008, China"]["Hunan Key Laboratory of Oral Health Research & Xiangya Stomatological Hospital & Xiangya School of Stomatology, Central South University, Changsha 410008, China"]["Department of Cell and Cancer Biology, University of Toledo College of Medicine and Life Sciences, Toledo, OH 43614, USA"]["Department of Biochemistry and Molecular Biology, Hunan Province Key Laboratory of Basic and Applied Hematology & Hunan Key Laboratory of Animal Models for Human Diseases, School of Life Sciences, Central South University, Changsha 410008, China"]Ginsenoside Rh2 is one of rare panaxidiols extracted from Panax ginseng and a potential estrogen receptor ligand that exhibits moderate estrogenic activity. However, the effect of Rh2 on growth inhibition and its underlying molecular mechanism in human breast cells are not fully understood. In this study, we tested cell viability by MTT and colony formation assays. Cell growth and cell cycle were determined to investigate the effect of ginsenoside Rh2 by flow cytometry. The expressions of estrogen receptors (ERs), TNFα, and apoptosis-related proteins were detected by qPCR and western blot analysis. The mechanisms of ERα and ERβ action were determined using transfection and inhibitors. Antitumor effect of ginsenoside Rh2 against MCF-7 cells was investigated in xenograft mice. Our results showed that ginsenoside Rh2 induced apoptosis and G1/S phase arrest in MCF-7 cells. Treatment of cells with ginsenoside Rh2 down-regulated protein levels of ERα, and up-regulated mRNA and protein levels of ERβ and TNFα. We also found that ginsenoside Rh2-induced TNFα over-expression is through up-regulation of ERβ initiated by ginsenoside Rh2. Furthermore, ginsenoside Rh2 induced MCF-7 cell apoptosis via estrogen receptor β-TNFα pathway in vivo. These results demonstrate that ginsenoside Rh2 promotes TNFα-induced apoptosis and G1/S phase arrest via regulation of ERβ.https://www.sciengine.com/doi/10.3724/abbs.2022039ginsenoside Rh2estrogen receptorTNFαbreast cancerapoptosis |
| spellingShingle | Peng Kunjian Luo Tiao Li Jijia Huang Jingjia Dong Zizeng Liu Jia Pi Chaoqiong Zou Zizeng Gu Qin Liu Ousheng Zhang Jian-Ting Luo Zhi-Yong Ginsenoside Rh2 inhibits breast cancer cell growth via<?A3B2 ACK?>ERβ-TNFα pathway Acta Biochimica et Biophysica Sinica ginsenoside Rh2 estrogen receptor TNFα breast cancer apoptosis |
| title | Ginsenoside Rh2 inhibits breast cancer cell growth via<?A3B2 ACK?>ERβ-TNFα pathway |
| title_full | Ginsenoside Rh2 inhibits breast cancer cell growth via<?A3B2 ACK?>ERβ-TNFα pathway |
| title_fullStr | Ginsenoside Rh2 inhibits breast cancer cell growth via<?A3B2 ACK?>ERβ-TNFα pathway |
| title_full_unstemmed | Ginsenoside Rh2 inhibits breast cancer cell growth via<?A3B2 ACK?>ERβ-TNFα pathway |
| title_short | Ginsenoside Rh2 inhibits breast cancer cell growth via<?A3B2 ACK?>ERβ-TNFα pathway |
| title_sort | ginsenoside rh2 inhibits breast cancer cell growth via a3b2 ack erβ tnfα pathway |
| topic | ginsenoside Rh2 estrogen receptor TNFα breast cancer apoptosis |
| url | https://www.sciengine.com/doi/10.3724/abbs.2022039 |
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