Efficient regeneration protocol for producing true-to-type oil palm (Elaeis guineensis (jacq.) through somatic embryogenesis from immature male inflorescence
Producing homogeneous planting material in oil palm poses a significant challenge, which can be addressed through somatic embryogenesis. This study successfully achieved somatic embryogenesis using immature male inflorescence from Tenera hybrid. Modified Eeuwens medium yielded better results than th...
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2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402417510X |
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| author | B. Kalyana Babu R.K. Mathur K. Suresh G. Ravichandran B. Susanthi G.B. Patil Narayana Ruthweek M. Mahesh |
| author_facet | B. Kalyana Babu R.K. Mathur K. Suresh G. Ravichandran B. Susanthi G.B. Patil Narayana Ruthweek M. Mahesh |
| author_sort | B. Kalyana Babu |
| collection | DOAJ |
| description | Producing homogeneous planting material in oil palm poses a significant challenge, which can be addressed through somatic embryogenesis. This study successfully achieved somatic embryogenesis using immature male inflorescence from Tenera hybrid. Modified Eeuwens medium yielded better results than the Murashige and Skoog (MS) and CHU (N6) media when supplemented with 2,4-D, picloram and α-naphthaleneacetic acid (NAA). The Eeuwens (Y3) medium supplemented with picloram led to an increased callus percentage, ranging from 25 % to 63.7 %. The highest rate of somatic embryogenesis was observed with a combination of 2,4-D (16 mg/l), picloram (24 mg/l), and 2,4,5-trichlorophenoxyacetic acid (2,4,5 –T) (2 mg/l), resulting in an average embryogenesis percentage between 2.54 % and 7.24 %. The average embryogenesis percentage varied from. Additionally, the liquid media proved effective for root growth and proliferation (R2 medium), which included Indole-3-butyric acid (IBA) (2 mg/l) and NAA (0.5 mg/l), along with activated charcoal (100 mg/l). The elongated, nodular-shaped root-like structures developed into root primordial structures. This protocol will be instrumental in producing homogeneous oil palm planting material through micropropagation and cloning of mother palms, facilitating the establishment of semi-clonal seed gardens. |
| format | Article |
| id | doaj-art-fe9414eb5349402c9509f597fc9b558b |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-fe9414eb5349402c9509f597fc9b558b2025-01-17T04:51:24ZengElsevierHeliyon2405-84402025-01-01111e41479Efficient regeneration protocol for producing true-to-type oil palm (Elaeis guineensis (jacq.) through somatic embryogenesis from immature male inflorescenceB. Kalyana Babu0R.K. Mathur1K. Suresh2G. Ravichandran3B. Susanthi4G.B. Patil5Narayana Ruthweek6M. Mahesh7Corresponding author.; ICAR-Indian Institute of Oil Palm Research, Pedavegi, 534 435, Eluru (Dt), Andhra Pradesh, IndiaICAR-Indian Institute of Oil Palm Research, Pedavegi, 534 435, Eluru (Dt), Andhra Pradesh, IndiaICAR-Indian Institute of Oil Palm Research, Pedavegi, 534 435, Eluru (Dt), Andhra Pradesh, IndiaICAR-Indian Institute of Oil Palm Research, Pedavegi, 534 435, Eluru (Dt), Andhra Pradesh, IndiaICAR-Indian Institute of Oil Palm Research, Pedavegi, 534 435, Eluru (Dt), Andhra Pradesh, IndiaICAR-Indian Institute of Oil Palm Research, Pedavegi, 534 435, Eluru (Dt), Andhra Pradesh, IndiaICAR-Indian Institute of Oil Palm Research, Pedavegi, 534 435, Eluru (Dt), Andhra Pradesh, IndiaICAR-Indian Institute of Oil Palm Research, Pedavegi, 534 435, Eluru (Dt), Andhra Pradesh, IndiaProducing homogeneous planting material in oil palm poses a significant challenge, which can be addressed through somatic embryogenesis. This study successfully achieved somatic embryogenesis using immature male inflorescence from Tenera hybrid. Modified Eeuwens medium yielded better results than the Murashige and Skoog (MS) and CHU (N6) media when supplemented with 2,4-D, picloram and α-naphthaleneacetic acid (NAA). The Eeuwens (Y3) medium supplemented with picloram led to an increased callus percentage, ranging from 25 % to 63.7 %. The highest rate of somatic embryogenesis was observed with a combination of 2,4-D (16 mg/l), picloram (24 mg/l), and 2,4,5-trichlorophenoxyacetic acid (2,4,5 –T) (2 mg/l), resulting in an average embryogenesis percentage between 2.54 % and 7.24 %. The average embryogenesis percentage varied from. Additionally, the liquid media proved effective for root growth and proliferation (R2 medium), which included Indole-3-butyric acid (IBA) (2 mg/l) and NAA (0.5 mg/l), along with activated charcoal (100 mg/l). The elongated, nodular-shaped root-like structures developed into root primordial structures. This protocol will be instrumental in producing homogeneous oil palm planting material through micropropagation and cloning of mother palms, facilitating the establishment of semi-clonal seed gardens.http://www.sciencedirect.com/science/article/pii/S240584402417510XOil palmSomatic embryogenesisTissue cultureImmature male inflorescenceTenera |
| spellingShingle | B. Kalyana Babu R.K. Mathur K. Suresh G. Ravichandran B. Susanthi G.B. Patil Narayana Ruthweek M. Mahesh Efficient regeneration protocol for producing true-to-type oil palm (Elaeis guineensis (jacq.) through somatic embryogenesis from immature male inflorescence Heliyon Oil palm Somatic embryogenesis Tissue culture Immature male inflorescence Tenera |
| title | Efficient regeneration protocol for producing true-to-type oil palm (Elaeis guineensis (jacq.) through somatic embryogenesis from immature male inflorescence |
| title_full | Efficient regeneration protocol for producing true-to-type oil palm (Elaeis guineensis (jacq.) through somatic embryogenesis from immature male inflorescence |
| title_fullStr | Efficient regeneration protocol for producing true-to-type oil palm (Elaeis guineensis (jacq.) through somatic embryogenesis from immature male inflorescence |
| title_full_unstemmed | Efficient regeneration protocol for producing true-to-type oil palm (Elaeis guineensis (jacq.) through somatic embryogenesis from immature male inflorescence |
| title_short | Efficient regeneration protocol for producing true-to-type oil palm (Elaeis guineensis (jacq.) through somatic embryogenesis from immature male inflorescence |
| title_sort | efficient regeneration protocol for producing true to type oil palm elaeis guineensis jacq through somatic embryogenesis from immature male inflorescence |
| topic | Oil palm Somatic embryogenesis Tissue culture Immature male inflorescence Tenera |
| url | http://www.sciencedirect.com/science/article/pii/S240584402417510X |
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