Establishment of a method for detecting complement C3d-sensitized platelets
[Objective] To establish a detection method for complement C3d-sensitized platelets. [Methods] Parallel detection of the same platelet sample under conditions of complement C3d sensitization and non-sensitization was conducted using microcolumn gel immunoagglutination inhibition assay technology. Th...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Institute of Blood Transfusion of Chinese Academy of Medical Sciences
2024-12-01
|
| Series: | Zhongguo shuxue zazhi |
| Subjects: | |
| Online Access: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2024.12.012&lang=en |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841557886946246656 |
|---|---|
| author | LI Hongyang YU Hongmei WANG Changmin LIU Tiemei |
| author_facet | LI Hongyang YU Hongmei WANG Changmin LIU Tiemei |
| author_sort | LI Hongyang |
| collection | DOAJ |
| description | [Objective] To establish a detection method for complement C3d-sensitized platelets. [Methods] Parallel detection of the same platelet sample under conditions of complement C3d sensitization and non-sensitization was conducted using microcolumn gel immunoagglutination inhibition assay technology. The supernatant obtained after the reaction between anti-C3d monoclonal antibodies and platelet samples was then reacted with C3d-sensitized red blood cells to observe whether agglutination occurs. Subsequently, this method was used to test samples from 22 clinical patients to determine whether their platelets were sensitized by complement C3d. [Results] The same platelet sample, after being sensitized with complement C3d, showed negative or weakened aggregation, which was determined as a positive result, whereas platelets that were not sensitized with complement C3d exhibited aggregation, which was determined as a negative result. A total of 22 clinical patient samples were tested, of which 16 were negative and 6 were positive. [Conclusion] A microcolumn gel immunoagglutination inhibition test was established to detect whether platelets are sensitized by complement C3d, which aids in the auxiliary diagnosis of complement-related immune diseases involving platelets. |
| format | Article |
| id | doaj-art-fe452f4c352a4ecaabae1a1d250b1de1 |
| institution | Kabale University |
| issn | 1004-549X |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | Institute of Blood Transfusion of Chinese Academy of Medical Sciences |
| record_format | Article |
| series | Zhongguo shuxue zazhi |
| spelling | doaj-art-fe452f4c352a4ecaabae1a1d250b1de12025-01-06T09:43:02ZzhoInstitute of Blood Transfusion of Chinese Academy of Medical SciencesZhongguo shuxue zazhi1004-549X2024-12-0137121412141610.13303/j.cjbt.issn.1004-549x.2024.12.0121004-549X(2024)12-1412-05Establishment of a method for detecting complement C3d-sensitized plateletsLI Hongyang0YU Hongmei1WANG Changmin2LIU Tiemei3Department of Blood Transfusion, China-Japan Union Hospital of Jilin University, Changchun 130033, ChinaDepartment of Blood Transfusion, China-Japan Union Hospital of Jilin University, Changchun 130033, ChinaChangchun Boxun Biotechnology Co., Ltd, Changchun 130033, ChinaDepartment of Blood Transfusion, China-Japan Union Hospital of Jilin University, Changchun 130033, China[Objective] To establish a detection method for complement C3d-sensitized platelets. [Methods] Parallel detection of the same platelet sample under conditions of complement C3d sensitization and non-sensitization was conducted using microcolumn gel immunoagglutination inhibition assay technology. The supernatant obtained after the reaction between anti-C3d monoclonal antibodies and platelet samples was then reacted with C3d-sensitized red blood cells to observe whether agglutination occurs. Subsequently, this method was used to test samples from 22 clinical patients to determine whether their platelets were sensitized by complement C3d. [Results] The same platelet sample, after being sensitized with complement C3d, showed negative or weakened aggregation, which was determined as a positive result, whereas platelets that were not sensitized with complement C3d exhibited aggregation, which was determined as a negative result. A total of 22 clinical patient samples were tested, of which 16 were negative and 6 were positive. [Conclusion] A microcolumn gel immunoagglutination inhibition test was established to detect whether platelets are sensitized by complement C3d, which aids in the auxiliary diagnosis of complement-related immune diseases involving platelets.https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2024.12.012&lang=enmicrocolumn gelimmunoaggregation inhibition assaycomplement c3dplatelets |
| spellingShingle | LI Hongyang YU Hongmei WANG Changmin LIU Tiemei Establishment of a method for detecting complement C3d-sensitized platelets Zhongguo shuxue zazhi microcolumn gel immunoaggregation inhibition assay complement c3d platelets |
| title | Establishment of a method for detecting complement C3d-sensitized platelets |
| title_full | Establishment of a method for detecting complement C3d-sensitized platelets |
| title_fullStr | Establishment of a method for detecting complement C3d-sensitized platelets |
| title_full_unstemmed | Establishment of a method for detecting complement C3d-sensitized platelets |
| title_short | Establishment of a method for detecting complement C3d-sensitized platelets |
| title_sort | establishment of a method for detecting complement c3d sensitized platelets |
| topic | microcolumn gel immunoaggregation inhibition assay complement c3d platelets |
| url | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2024.12.012&lang=en |
| work_keys_str_mv | AT lihongyang establishmentofamethodfordetectingcomplementc3dsensitizedplatelets AT yuhongmei establishmentofamethodfordetectingcomplementc3dsensitizedplatelets AT wangchangmin establishmentofamethodfordetectingcomplementc3dsensitizedplatelets AT liutiemei establishmentofamethodfordetectingcomplementc3dsensitizedplatelets |