Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in China
Introduction: There are few studies on paediatric carbapenem-resistant Klebsiella pneumoniae (CRKP) in China. The present study investigated the molecular epidemiological and drug resistance characteristics of CRKP from paediatric patients in China to provide a reference for the prevention and cont...
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The Journal of Infection in Developing Countries
2022-11-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/17003 |
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| author | Xian Zhang Jian Xue Mei-Jing Shen Wen-Hong An Ze-Qiong Chen Kai-Feng Wu |
| author_facet | Xian Zhang Jian Xue Mei-Jing Shen Wen-Hong An Ze-Qiong Chen Kai-Feng Wu |
| author_sort | Xian Zhang |
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Introduction: There are few studies on paediatric carbapenem-resistant Klebsiella pneumoniae (CRKP) in China. The present study investigated the molecular epidemiological and drug resistance characteristics of CRKP from paediatric patients in China to provide a reference for the prevention and control of CRKP infection.
Methodology: In total, 20 nonrepetitive clinical CRKP isolates were collected between February 2019 and February 2020 in a tertiary hospital in China. Strain identification and drug susceptibility testing were carried out using the VITEK® 2 Compact Bacterial Identification and Monitoring System. Sequence typing, phylogenetic relationships, and antibiotic resistance-associated genes were analysed by whole genome sequencing (WGS).
Results: sequence typing (MLST) and Core genome multilocus sequence typing (cgMLST) analysis revealed the most frequently represented were ST2407-CT3536 (30%), ST76-CT5893 (25%), and ST309-CT7864 (25%). All 20 CRKP isolates were divided into three clusters. All isolates were highly resistant to a variety of β-lactams and were highly susceptible to quinolones, aminoglycosides, and sulphonamides. All isolates mainly carried the carbapenem resistance genes blaNDM-1 and blaKPC-2, among which 10 isolates carried both blaNDM-1 and blaKPC-2 simultaneously.
Conclusions: Sequence typing, phylogenetic relationships, and antibiotic resistance genes can be determined using WGS technology. This can guide CRKP infection control and clinical treatment for paediatric patients.
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| format | Article |
| id | doaj-art-fbdfa3ad24b64dbaa9fa8f179d6e230f |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-fbdfa3ad24b64dbaa9fa8f179d6e230f2025-08-20T03:52:39ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802022-11-01161110.3855/jidc.17003Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in ChinaXian Zhang0Jian Xue1Mei-Jing Shen2Wen-Hong An3Ze-Qiong Chen4Kai-Feng Wu5Department of Hospital Infection, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, ChinaDepartment of Health Management, Zunyi Medical And Pharmaceutical College, Guizhou, ChinaDepartment of Clinical Laboratory, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, ChinaDepartment of Hospital Infection, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, ChinaDepartment of Hospital Infection, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, ChinaDepartment of Clinical Laboratory, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, China Introduction: There are few studies on paediatric carbapenem-resistant Klebsiella pneumoniae (CRKP) in China. The present study investigated the molecular epidemiological and drug resistance characteristics of CRKP from paediatric patients in China to provide a reference for the prevention and control of CRKP infection. Methodology: In total, 20 nonrepetitive clinical CRKP isolates were collected between February 2019 and February 2020 in a tertiary hospital in China. Strain identification and drug susceptibility testing were carried out using the VITEK® 2 Compact Bacterial Identification and Monitoring System. Sequence typing, phylogenetic relationships, and antibiotic resistance-associated genes were analysed by whole genome sequencing (WGS). Results: sequence typing (MLST) and Core genome multilocus sequence typing (cgMLST) analysis revealed the most frequently represented were ST2407-CT3536 (30%), ST76-CT5893 (25%), and ST309-CT7864 (25%). All 20 CRKP isolates were divided into three clusters. All isolates were highly resistant to a variety of β-lactams and were highly susceptible to quinolones, aminoglycosides, and sulphonamides. All isolates mainly carried the carbapenem resistance genes blaNDM-1 and blaKPC-2, among which 10 isolates carried both blaNDM-1 and blaKPC-2 simultaneously. Conclusions: Sequence typing, phylogenetic relationships, and antibiotic resistance genes can be determined using WGS technology. This can guide CRKP infection control and clinical treatment for paediatric patients. https://jidc.org/index.php/journal/article/view/17003Whole genome sequencingpaediatricscarbapenem-resistant Klebsiella pneumoniaemolecular typingdrug resistance |
| spellingShingle | Xian Zhang Jian Xue Mei-Jing Shen Wen-Hong An Ze-Qiong Chen Kai-Feng Wu Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in China Journal of Infection in Developing Countries Whole genome sequencing paediatrics carbapenem-resistant Klebsiella pneumoniae molecular typing drug resistance |
| title | Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in China |
| title_full | Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in China |
| title_fullStr | Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in China |
| title_full_unstemmed | Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in China |
| title_short | Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in China |
| title_sort | molecular typing and drug resistance analysis of carbapenem resistant klebsiella pneumoniae from paediatric patients in china |
| topic | Whole genome sequencing paediatrics carbapenem-resistant Klebsiella pneumoniae molecular typing drug resistance |
| url | https://jidc.org/index.php/journal/article/view/17003 |
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