Evaluation of the protective role of ellagic acid in retinal degeneration: an experimental model
Background/aim Ellagic acid (EA) is widely recognized as a natural compound with pharmacological potency as a polyphenolic molecule, possessing antioxidant, anti-inflammatory, antimutagenic, and antiproliferative characteristics. The present study aims to evaluate the potential neuroprotective effec...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Journal of the Arab Society for Medical Research |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/jasmr.jasmr_25_24 |
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| author | Eman E. Mousa Aziza A. Elsaied Mohammad M. Murad Amal E. Ibrahim |
| author_facet | Eman E. Mousa Aziza A. Elsaied Mohammad M. Murad Amal E. Ibrahim |
| author_sort | Eman E. Mousa |
| collection | DOAJ |
| description | Background/aim
Ellagic acid (EA) is widely recognized as a natural compound with pharmacological potency as a polyphenolic molecule, possessing antioxidant, anti-inflammatory, antimutagenic, and antiproliferative characteristics. The present study aims to evaluate the potential neuroprotective effect of EA in retinal degeneration induced experimentally in rabbits.
Material and methods
A total of 27 male white New Zealand rabbits, with an average weight ranging from 1.5 to 2 kg, were divided into three groups (nine each). Group I served as the control group, while group II and group III, the macular degeneration (MD) induction groups that received a single intravitreal injection of sodium iodate (SI). Following the injection, group III was given 50 mg/kg of EA powder for 21 days, starting immediately after MD induction. Ophthalmic examinations of the retinas were conducted on days 7, 14, and 21 using a fundus camera, followed by electroretinogram (ERG) recording after MD induction. Apoptotic caspase-3 and caspase-7 activities in the retina tissues were also measured postdecapitation.
Results
The results showed a significant decrease (P≤0.05) in electroretinogram ‘a- and b-waves’ following intravitreal SI injection in group II and III comparing with control group. The pattern of internucleosomal fragmentation, indicating apoptosis, was time-dependent. The injection also increased relative caspase-3 and caspase-7 activity. However, group III of rabbits that is treated with EA exhibited noticeable improvements in these outcomes in comparing with group of MD-induced rabbits.
Conclusions
The administration of EA demonstrated a notable impact in improving the retina’s function, and decreased the apoptosis levels in MD model of rabbits |
| format | Article |
| id | doaj-art-fa0aa176b18b4c60a6c138f0fba9e7b7 |
| institution | Kabale University |
| issn | 1687-4293 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of the Arab Society for Medical Research |
| spelling | doaj-art-fa0aa176b18b4c60a6c138f0fba9e7b72025-01-04T14:00:31ZengWolters Kluwer Medknow PublicationsJournal of the Arab Society for Medical Research1687-42932024-12-0119221122010.4103/jasmr.jasmr_25_24Evaluation of the protective role of ellagic acid in retinal degeneration: an experimental modelEman E. MousaAziza A. ElsaiedMohammad M. MuradAmal E. IbrahimBackground/aim Ellagic acid (EA) is widely recognized as a natural compound with pharmacological potency as a polyphenolic molecule, possessing antioxidant, anti-inflammatory, antimutagenic, and antiproliferative characteristics. The present study aims to evaluate the potential neuroprotective effect of EA in retinal degeneration induced experimentally in rabbits. Material and methods A total of 27 male white New Zealand rabbits, with an average weight ranging from 1.5 to 2 kg, were divided into three groups (nine each). Group I served as the control group, while group II and group III, the macular degeneration (MD) induction groups that received a single intravitreal injection of sodium iodate (SI). Following the injection, group III was given 50 mg/kg of EA powder for 21 days, starting immediately after MD induction. Ophthalmic examinations of the retinas were conducted on days 7, 14, and 21 using a fundus camera, followed by electroretinogram (ERG) recording after MD induction. Apoptotic caspase-3 and caspase-7 activities in the retina tissues were also measured postdecapitation. Results The results showed a significant decrease (P≤0.05) in electroretinogram ‘a- and b-waves’ following intravitreal SI injection in group II and III comparing with control group. The pattern of internucleosomal fragmentation, indicating apoptosis, was time-dependent. The injection also increased relative caspase-3 and caspase-7 activity. However, group III of rabbits that is treated with EA exhibited noticeable improvements in these outcomes in comparing with group of MD-induced rabbits. Conclusions The administration of EA demonstrated a notable impact in improving the retina’s function, and decreased the apoptosis levels in MD model of rabbitshttps://journals.lww.com/10.4103/jasmr.jasmr_25_24apoptosiselectroretinogramellagic acidsodium iodaterabbitretina |
| spellingShingle | Eman E. Mousa Aziza A. Elsaied Mohammad M. Murad Amal E. Ibrahim Evaluation of the protective role of ellagic acid in retinal degeneration: an experimental model Journal of the Arab Society for Medical Research apoptosis electroretinogram ellagic acid sodium iodate rabbit retina |
| title | Evaluation of the protective role of ellagic acid in retinal degeneration: an experimental model |
| title_full | Evaluation of the protective role of ellagic acid in retinal degeneration: an experimental model |
| title_fullStr | Evaluation of the protective role of ellagic acid in retinal degeneration: an experimental model |
| title_full_unstemmed | Evaluation of the protective role of ellagic acid in retinal degeneration: an experimental model |
| title_short | Evaluation of the protective role of ellagic acid in retinal degeneration: an experimental model |
| title_sort | evaluation of the protective role of ellagic acid in retinal degeneration an experimental model |
| topic | apoptosis electroretinogram ellagic acid sodium iodate rabbit retina |
| url | https://journals.lww.com/10.4103/jasmr.jasmr_25_24 |
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