Development of a qPCR and a Lamp Assay for Verticillium longisporum Detection and a Triplex qPCR Assay for Simultaneous Detection of V. longisporum, Leptosphaeria biglobosa, and L. maculans from Canola Samples

Development of a qPCR and a Lamp Assay for Verticillium longisporum Detection and a Triplex qPCR Assay for Simultaneous Detection of V. longisporum, Leptosphaeria biglobosa, and L. maculans from Canola Samples

Verticillium wilt, Verticillium stem striping, and Verticillium stripe are common disease names that all denote infection caused by Verticillium longisporum on canola or other brassica crops. In this study, a quantitative PCR (qPCR) and a loop-mediated isothermal amplification (LAMP) assay were deve...

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Bibliographic Details
Main Authors: Heting Fu, Yalong Yang, Junye Jiang, Greg C. Daniels, Blake Hill, Shiming Xue, Kher Zahr, L. Stellar, Michael W. Harding, David Feindel, Carol Bvindi, W. G. Dilantha Fernando, Lipu Wang, Jie Feng
Format: Article
Language:English
Published: The American Phytopathological Society 2024-12-01
Series:PhytoFrontiers
Subjects:
blackleg
canola
multiplex qPCR
stripe
Verticillium stem striping
wilt
Online Access:https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-03-24-0031-R
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https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-03-24-0031-R

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