Structural and dynamic impacts of single-atom disruptions to guide RNA interactions within the recognition lobe of Geobacillus stearothermophilus Cas9

Structural and dynamic impacts of single-atom disruptions to guide RNA interactions within the recognition lobe of Geobacillus stearothermophilus Cas9

The intuitive manipulation of specific amino acids to alter the activity or specificity of CRISPR-Cas9 has been a topic of great interest. As a large multi-domain RNA-guided endonuclease, the intricate molecular crosstalk within the Cas9 protein hinges on its conformational dynamics, but a comprehen...

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Bibliographic Details
Main Authors: Helen B Belato, Alexa L Knight, Alexandra M D'Ordine, Chinmai Pindi, Zhiqiang Fan, Jinping Luo, Giulia Palermo, Gerwald Jogl, George P Lisi
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2025-05-01
Series:eLife
Subjects:
allostery
CRISPR-Cas9
NMR spectroscopy
molecular dynamics
specificity
Online Access:https://elifesciences.org/articles/99275
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https://elifesciences.org/articles/99275

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