ROS Scavenging and Osteogenic Differentiation Potential of L-Methionine-Substituted Poly(Organophosphazene) Electrospun Fibers
This study investigated the application of poly[bis (ethylmethionato) phosphazene] (PαAPz-M) electrospun fibers in tissue engineering, focusing on their reactive oxygen species (ROS) scavenging capabilities and material-directed cell behavior, including the influence of their degradation products on...
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MDPI AG
2024-11-01
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| Series: | Biomimetics |
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| Online Access: | https://www.mdpi.com/2313-7673/9/11/676 |
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| author | Meng Wang Kibret Mequanint |
| author_facet | Meng Wang Kibret Mequanint |
| author_sort | Meng Wang |
| collection | DOAJ |
| description | This study investigated the application of poly[bis (ethylmethionato) phosphazene] (PαAPz-M) electrospun fibers in tissue engineering, focusing on their reactive oxygen species (ROS) scavenging capabilities and material-directed cell behavior, including the influence of their degradation products on cell viability and differentiation, and the scaffold topography’s influence on cell alignment. The ROS scavenging ability of PαAPz-M was assessed by DPPH assay, and then PαAPz-M’s protection against exogenous ROS was studied. The results showed enhanced cell viability on PαAPz-M fiber mats under oxidative stress conditions. This study also investigated the effects of the degradation products of PαAPz-M on cell viability and osteogenic differentiation. It was observed that the late-stage degradation product, phosphoric acid, can significantly influence the osteogenic differentiation of MSCs. In contrast, methionine, which is the early-stage degradation product, showed a minimal influence. Additionally, the study fabricated fiber mats that can lead to enhanced cell alignment while maintaining high porosity. Collectively, this study expanded the applications of PαAPz-M fiber mat protection against oxidative stress and guiding osteogenic differentiation and cell alignment. |
| format | Article |
| id | doaj-art-f96b1509847544c0a6cd5dbc20a829aa |
| institution | Kabale University |
| issn | 2313-7673 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Biomimetics |
| spelling | doaj-art-f96b1509847544c0a6cd5dbc20a829aa2024-11-26T17:53:40ZengMDPI AGBiomimetics2313-76732024-11-0191167610.3390/biomimetics9110676ROS Scavenging and Osteogenic Differentiation Potential of L-Methionine-Substituted Poly(Organophosphazene) Electrospun FibersMeng Wang0Kibret Mequanint1Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, ON N6A 5B9, CanadaDepartment of Chemical and Biochemical Engineering, The University of Western Ontario, London, ON N6A 5B9, CanadaThis study investigated the application of poly[bis (ethylmethionato) phosphazene] (PαAPz-M) electrospun fibers in tissue engineering, focusing on their reactive oxygen species (ROS) scavenging capabilities and material-directed cell behavior, including the influence of their degradation products on cell viability and differentiation, and the scaffold topography’s influence on cell alignment. The ROS scavenging ability of PαAPz-M was assessed by DPPH assay, and then PαAPz-M’s protection against exogenous ROS was studied. The results showed enhanced cell viability on PαAPz-M fiber mats under oxidative stress conditions. This study also investigated the effects of the degradation products of PαAPz-M on cell viability and osteogenic differentiation. It was observed that the late-stage degradation product, phosphoric acid, can significantly influence the osteogenic differentiation of MSCs. In contrast, methionine, which is the early-stage degradation product, showed a minimal influence. Additionally, the study fabricated fiber mats that can lead to enhanced cell alignment while maintaining high porosity. Collectively, this study expanded the applications of PαAPz-M fiber mat protection against oxidative stress and guiding osteogenic differentiation and cell alignment.https://www.mdpi.com/2313-7673/9/11/676biodegradable poly(organophosphazenes)ROS scavengermaterial-guided cell behaviorhuman mesenchymal stem cellsvascular smooth muscle cellsvascular tissue engineering |
| spellingShingle | Meng Wang Kibret Mequanint ROS Scavenging and Osteogenic Differentiation Potential of L-Methionine-Substituted Poly(Organophosphazene) Electrospun Fibers Biomimetics biodegradable poly(organophosphazenes) ROS scavenger material-guided cell behavior human mesenchymal stem cells vascular smooth muscle cells vascular tissue engineering |
| title | ROS Scavenging and Osteogenic Differentiation Potential of L-Methionine-Substituted Poly(Organophosphazene) Electrospun Fibers |
| title_full | ROS Scavenging and Osteogenic Differentiation Potential of L-Methionine-Substituted Poly(Organophosphazene) Electrospun Fibers |
| title_fullStr | ROS Scavenging and Osteogenic Differentiation Potential of L-Methionine-Substituted Poly(Organophosphazene) Electrospun Fibers |
| title_full_unstemmed | ROS Scavenging and Osteogenic Differentiation Potential of L-Methionine-Substituted Poly(Organophosphazene) Electrospun Fibers |
| title_short | ROS Scavenging and Osteogenic Differentiation Potential of L-Methionine-Substituted Poly(Organophosphazene) Electrospun Fibers |
| title_sort | ros scavenging and osteogenic differentiation potential of l methionine substituted poly organophosphazene electrospun fibers |
| topic | biodegradable poly(organophosphazenes) ROS scavenger material-guided cell behavior human mesenchymal stem cells vascular smooth muscle cells vascular tissue engineering |
| url | https://www.mdpi.com/2313-7673/9/11/676 |
| work_keys_str_mv | AT mengwang rosscavengingandosteogenicdifferentiationpotentialoflmethioninesubstitutedpolyorganophosphazeneelectrospunfibers AT kibretmequanint rosscavengingandosteogenicdifferentiationpotentialoflmethioninesubstitutedpolyorganophosphazeneelectrospunfibers |