Femtomolar hydrogen sulfide detection via hybrid small-molecule nano-arrays
Abstract Early disease diagnosis hinges on the sensitive detection of signaling molecules. Among these, hydrogen sulfide (H2S) has emerged as a critical player in cardiovascular and nervous system signaling. On-chip immunoassays, particularly nanoarray-based interfacial detection, offer promising av...
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| Format: | Article |
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Nature Portfolio
2024-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55123-y |
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| author | Xing Xing Luyan Wu Yuchen Zhang Jiahao Pan Yusuke Ishigaki Huaqing Xie Takanori Suzuki Deju Ye Jianhua Zhang Weihua Zhang Zhenda Lu |
| author_facet | Xing Xing Luyan Wu Yuchen Zhang Jiahao Pan Yusuke Ishigaki Huaqing Xie Takanori Suzuki Deju Ye Jianhua Zhang Weihua Zhang Zhenda Lu |
| author_sort | Xing Xing |
| collection | DOAJ |
| description | Abstract Early disease diagnosis hinges on the sensitive detection of signaling molecules. Among these, hydrogen sulfide (H2S) has emerged as a critical player in cardiovascular and nervous system signaling. On-chip immunoassays, particularly nanoarray-based interfacial detection, offer promising avenues for ultra-sensitive analysis due to their confined reaction volumes and precise signal localization. Beyond the DNA or protein biomolecules array, this work presents a promising hybrid small molecule nano-array for H2S detection, using the power of dual molecules: a dye for fluorescence emission and a quencher with specific H2S reactivity. Upon H2S interaction, the quenched fluorescence reignites, creating an easily detectable array of bright spots. The molecule nano-array sensor shows exceptional responses to H2S over 8 magnitudes of dynamic range from 1 fM to 0.1 μM, with a remarkable detection limit of 1 fM, just using a 10 μL solution. This H2S detection method has the potential to significantly improve bioassay platforms, and the hybrid small-molecule nano-arrays we developed could be a valuable tool for advancing signaling molecule detection. |
| format | Article |
| id | doaj-art-f8cb03d732f04f38aab6a49d0f176254 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-f8cb03d732f04f38aab6a49d0f1762542025-01-05T12:35:22ZengNature PortfolioNature Communications2041-17232024-12-011511810.1038/s41467-024-55123-yFemtomolar hydrogen sulfide detection via hybrid small-molecule nano-arraysXing Xing0Luyan Wu1Yuchen Zhang2Jiahao Pan3Yusuke Ishigaki4Huaqing Xie5Takanori Suzuki6Deju Ye7Jianhua Zhang8Weihua Zhang9Zhenda Lu10College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing UniversityState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing UniversityCollege of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing UniversityCollege of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing UniversityDepartment of Chemistry, Faculty of Science, Hokkaido UniversitySchool of Energy and Materials, Shanghai Thermophysical Properties Big Data Professional Technical Service Platform, Shanghai Engineering Research Center of Advanced Thermal Functional Materials, Shanghai Polytechnic UniversityDepartment of Chemistry, Faculty of Science, Hokkaido UniversityState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing UniversityN.O.D topia (GuangZhou) Biotechnology Co., Ltd., Simpcare (GuangZhou) Biotechnology Co. Ltd.College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing UniversityCollege of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing UniversityAbstract Early disease diagnosis hinges on the sensitive detection of signaling molecules. Among these, hydrogen sulfide (H2S) has emerged as a critical player in cardiovascular and nervous system signaling. On-chip immunoassays, particularly nanoarray-based interfacial detection, offer promising avenues for ultra-sensitive analysis due to their confined reaction volumes and precise signal localization. Beyond the DNA or protein biomolecules array, this work presents a promising hybrid small molecule nano-array for H2S detection, using the power of dual molecules: a dye for fluorescence emission and a quencher with specific H2S reactivity. Upon H2S interaction, the quenched fluorescence reignites, creating an easily detectable array of bright spots. The molecule nano-array sensor shows exceptional responses to H2S over 8 magnitudes of dynamic range from 1 fM to 0.1 μM, with a remarkable detection limit of 1 fM, just using a 10 μL solution. This H2S detection method has the potential to significantly improve bioassay platforms, and the hybrid small-molecule nano-arrays we developed could be a valuable tool for advancing signaling molecule detection.https://doi.org/10.1038/s41467-024-55123-y |
| spellingShingle | Xing Xing Luyan Wu Yuchen Zhang Jiahao Pan Yusuke Ishigaki Huaqing Xie Takanori Suzuki Deju Ye Jianhua Zhang Weihua Zhang Zhenda Lu Femtomolar hydrogen sulfide detection via hybrid small-molecule nano-arrays Nature Communications |
| title | Femtomolar hydrogen sulfide detection via hybrid small-molecule nano-arrays |
| title_full | Femtomolar hydrogen sulfide detection via hybrid small-molecule nano-arrays |
| title_fullStr | Femtomolar hydrogen sulfide detection via hybrid small-molecule nano-arrays |
| title_full_unstemmed | Femtomolar hydrogen sulfide detection via hybrid small-molecule nano-arrays |
| title_short | Femtomolar hydrogen sulfide detection via hybrid small-molecule nano-arrays |
| title_sort | femtomolar hydrogen sulfide detection via hybrid small molecule nano arrays |
| url | https://doi.org/10.1038/s41467-024-55123-y |
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