SWITCHER, a CRISPR-inducible floxed wild-type Cre regulating CRISPR activity

Abstract Although several Cre-regulated CRISPR/Cas platforms exist, a CRISPR/Cas-controlled Cre-system remains a challenge. Here, we present a genetic switch we term SWITCHER based on a floxed wild-type Cre-construct representing a CRISPR-inducible and self-limiting kill switch. By leveraging CRISPR...

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Bibliographic Details
Main Authors: Björn Schuster, Ivana Dobiášovská, Jovana Ćurčić, Petr Pajer, Šimon Borna, Petr Bartůněk
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-08427-8
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