Emerging technologies for single-cell glycomics
Glycans are present on virtually all cellular surfaces and are important regulators of multicellular communications. Advances in single-cell omics technologies have revolutionized life science research by elucidating cellular heterogeneity through integrated multimodal analyses, providing a comprehe...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
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| Series: | BBA Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667160324000139 |
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| Summary: | Glycans are present on virtually all cellular surfaces and are important regulators of multicellular communications. Advances in single-cell omics technologies have revolutionized life science research by elucidating cellular heterogeneity through integrated multimodal analyses, providing a comprehensive view of cellular functions. However, dissecting the heterogeneity of glycans at the single-cell level has been challenging due to their structural complexity and unamplifiable nature. Recently, we developed a novel technology called single-cell glycan and RNA sequencing (scGR-seq), which converts glycan information into genetic information using DNA-barcoded lectins, amplifies it by PCR, and simultaneously measures the glycome and transcriptome in thousands of single cells on a next-generation sequencer. In this mini-review, we review the recent advances in single-cell glycomics, focusing on our scGR-seq technology. |
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| ISSN: | 2667-1603 |