Effects of drugs on kinetic values of cytokines, adenosine deaminase and 13,14-dihydro-15-keto-prostaglandin f<sub>2α</sub> in endotoxemia: a different approach
Aim: The objective of this study was to determine the effects of enrofloxacin (ENR), flunixin meglumine (FM) and dexamethasone (DEX) on kinetic values of cytokines, adenosine deaminase (ADA) and 13,14-dihydro-15-keto-prostaglandin F<sub>2a</sub> (PGM) in lipopolysaccharide-induced endoto...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=553 |
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| Summary: | Aim: The objective of this study was to determine the effects
of enrofloxacin (ENR), flunixin meglumine (FM) and dexamethasone
(DEX) on kinetic values of cytokines, adenosine
deaminase (ADA) and 13,14-dihydro-15-keto-prostaglandin
F<sub>2a</sub> (PGM) in lipopolysaccharide-induced endotoxemia.
Materials and Methods: Rats were divided into seven
groups. To induce endotoxemia, lipopolysaccharide (LPS)
was injected into all groups, including the positive control.
The six other groups received the following drugs: ENR,
FM, low-dose DEX, high-dose DEX, ENR + FM + low-dose
DEX and ENR + FM + high-dose DEX. After the treatments,
blood samples were collected at 0, 1, 2, 4, 6, 8, 12, 24, and
48 hours. Serum tumor necrosis factor alpha (TNFα), interleukin-
6 (IL-6), interleukin-10 (IL-10), ADA and plasma
PGM levels were determined by ELISA. Area under the concentration
time curve (AUC<sub>0-48</sub>), maximal concentration in
plasma or serum (C<sub>max</sub>) and time to reach (t<sub>max</sub>) values were
determined by pharmacokinetic computer program.
Results: ENR increased (p<0.05) AUC<sub>0-48</sub> of PGM and decreased
(p<0.05) AUC<sub>0-48</sub> of IL-6, IL-10 and ADA, while FM
decreased (p<0.05) AUC<sub>0-48</sub> of IL-6 and ADA compared
to LPS group. DEX alone and combined administrations
caused the lower AUC<sub>0-48</sub> of all values (p<0.05).
Conclusion: Kinetic values, especially AUC, may be used
for total evaluation of endotoxemia markers determined at
different sampling times in same groups as a different and
logical approach. |
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| ISSN: | 1309-6958 2146-1953 |