Cowpea bean β-vignin-derived AQQSY peptide exerts an anticancer effect by inducing cell cycle arrest in the G0/G1 phase and modulating apoptotic signals
The anticancer effect of digested β-vignin and pure peptides from cowpea beans was investigated in cultivated human colon cancer cells. The effect of AQQSY on the cell cycle was similar to that of palbociclib, suggesting that AQQSY may function as a CDK-6-targeting agent. AQQSY peptide reduced the p...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-11-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624005000 |
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| author | Biane Oliveira Philadelpho Johnnie Elton Machado dos Santos Emily Elaine Davis Mariana Barros de Cerqueira e Silva Eduardo Maffud Cilli Ederlan de Souza Ferreira Elvira González de Mejia |
| author_facet | Biane Oliveira Philadelpho Johnnie Elton Machado dos Santos Emily Elaine Davis Mariana Barros de Cerqueira e Silva Eduardo Maffud Cilli Ederlan de Souza Ferreira Elvira González de Mejia |
| author_sort | Biane Oliveira Philadelpho |
| collection | DOAJ |
| description | The anticancer effect of digested β-vignin and pure peptides from cowpea beans was investigated in cultivated human colon cancer cells. The effect of AQQSY on the cell cycle was similar to that of palbociclib, suggesting that AQQSY may function as a CDK-6-targeting agent. AQQSY peptide reduced the p-Rb/Rb ratio and induced apoptosis through an increase of Bax/Bcl-2 ratio, a decrease of XIAP, and activation of caspase-3. VIPASY peptide showed the potential to induce apoptosis through an increase in Bax/Bcl-2 ratio. The combination of VIPASY and AQQSY with palbociclib caused an additive effect on cell inhibition. Treatment with selected peptides from β-vignin digest caused cell cycle arrest by upregulation of p16 and apoptosis by increasing Bax/Bcl-2 ratio and caspase-8. The peptides and β-vignin digest treatments were more selective for cancer cells than the drugs. Additional research is necessary in vivo to understand the mechanisms of cowpea β-vignin-derived peptides in CRC treatment. |
| format | Article |
| id | doaj-art-f74c3583a66143f0b9d2ae8af5908a9c |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-f74c3583a66143f0b9d2ae8af5908a9c2024-11-16T05:10:40ZengElsevierJournal of Functional Foods1756-46462024-11-01122106498Cowpea bean β-vignin-derived AQQSY peptide exerts an anticancer effect by inducing cell cycle arrest in the G0/G1 phase and modulating apoptotic signalsBiane Oliveira Philadelpho0Johnnie Elton Machado dos Santos1Emily Elaine Davis2Mariana Barros de Cerqueira e Silva3Eduardo Maffud Cilli4Ederlan de Souza Ferreira5Elvira González de Mejia6School of Pharmacy, Federal University of Bahia, Barão de Jeremoabo Street, 40170-115 Salvador, BA, Brazil; University of Illinois at Urbana-Champaign, Department Food Science and Human Nutrition, 228 Edward R Madigan Lab, Urbana, IL 61801, BrazilSchool of Pharmacy, Federal University of Bahia, Barão de Jeremoabo Street, 40170-115 Salvador, BA, BrazilUniversity of Illinois at Urbana-Champaign, Department Food Science and Human Nutrition, 228 Edward R Madigan Lab, Urbana, IL 61801, BrazilSchool of Pharmacy, Federal University of Amazonas, General Rodrigo Octávio Jordão Ramos Avenue, 69067-005, Manaus, Amazonas, BrazilInstitute of Chemistry, São Paulo State University (UNESP), Rua Prof. Francisco Degni, 14800-060, Araraquara, SP, BrazilSchool of Pharmacy, Federal University of Bahia, Barão de Jeremoabo Street, 40170-115 Salvador, BA, Brazil; Corresponding authors.University of Illinois at Urbana-Champaign, Department Food Science and Human Nutrition, 228 Edward R Madigan Lab, Urbana, IL 61801, Brazil; Corresponding authors.The anticancer effect of digested β-vignin and pure peptides from cowpea beans was investigated in cultivated human colon cancer cells. The effect of AQQSY on the cell cycle was similar to that of palbociclib, suggesting that AQQSY may function as a CDK-6-targeting agent. AQQSY peptide reduced the p-Rb/Rb ratio and induced apoptosis through an increase of Bax/Bcl-2 ratio, a decrease of XIAP, and activation of caspase-3. VIPASY peptide showed the potential to induce apoptosis through an increase in Bax/Bcl-2 ratio. The combination of VIPASY and AQQSY with palbociclib caused an additive effect on cell inhibition. Treatment with selected peptides from β-vignin digest caused cell cycle arrest by upregulation of p16 and apoptosis by increasing Bax/Bcl-2 ratio and caspase-8. The peptides and β-vignin digest treatments were more selective for cancer cells than the drugs. Additional research is necessary in vivo to understand the mechanisms of cowpea β-vignin-derived peptides in CRC treatment.http://www.sciencedirect.com/science/article/pii/S1756464624005000Vigna unguiculataBioactive peptidesDockingCell cycle arrestBax/Bcl-2. HCT-116 cells |
| spellingShingle | Biane Oliveira Philadelpho Johnnie Elton Machado dos Santos Emily Elaine Davis Mariana Barros de Cerqueira e Silva Eduardo Maffud Cilli Ederlan de Souza Ferreira Elvira González de Mejia Cowpea bean β-vignin-derived AQQSY peptide exerts an anticancer effect by inducing cell cycle arrest in the G0/G1 phase and modulating apoptotic signals Journal of Functional Foods Vigna unguiculata Bioactive peptides Docking Cell cycle arrest Bax/Bcl-2. HCT-116 cells |
| title | Cowpea bean β-vignin-derived AQQSY peptide exerts an anticancer effect by inducing cell cycle arrest in the G0/G1 phase and modulating apoptotic signals |
| title_full | Cowpea bean β-vignin-derived AQQSY peptide exerts an anticancer effect by inducing cell cycle arrest in the G0/G1 phase and modulating apoptotic signals |
| title_fullStr | Cowpea bean β-vignin-derived AQQSY peptide exerts an anticancer effect by inducing cell cycle arrest in the G0/G1 phase and modulating apoptotic signals |
| title_full_unstemmed | Cowpea bean β-vignin-derived AQQSY peptide exerts an anticancer effect by inducing cell cycle arrest in the G0/G1 phase and modulating apoptotic signals |
| title_short | Cowpea bean β-vignin-derived AQQSY peptide exerts an anticancer effect by inducing cell cycle arrest in the G0/G1 phase and modulating apoptotic signals |
| title_sort | cowpea bean β vignin derived aqqsy peptide exerts an anticancer effect by inducing cell cycle arrest in the g0 g1 phase and modulating apoptotic signals |
| topic | Vigna unguiculata Bioactive peptides Docking Cell cycle arrest Bax/Bcl-2. HCT-116 cells |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624005000 |
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