Imaging Multidrug Resistance P-glycoprotein Transport Function Using MicroPET with Technetium-94m-Sestamibi
The best characterized mechanism of multidrug resistance (MDR) in cancer involves the MDR1 efflux transporter P-glycoprotein (Pgp). The positron-emitting radiotracer hexakis (2-methoxyisobutylisonitrile)- 94m Tc ( 94m Tc-MIBI) was synthesized and validated in cell transport studies as a substrate fo...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2005-01-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200504166 |
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| _version_ | 1841564649558900736 |
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| author | Heather M. Bigott Julie L. Prior David R. Piwnica-Worms Michael J. Welch |
| author_facet | Heather M. Bigott Julie L. Prior David R. Piwnica-Worms Michael J. Welch |
| author_sort | Heather M. Bigott |
| collection | DOAJ |
| description | The best characterized mechanism of multidrug resistance (MDR) in cancer involves the MDR1 efflux transporter P-glycoprotein (Pgp). The positron-emitting radiotracer hexakis (2-methoxyisobutylisonitrile)- 94m Tc ( 94m Tc-MIBI) was synthesized and validated in cell transport studies as a substrate for MDR1 Pgp. In vivo small-scale PET imaging and biodistribution studies of mdr1a/1b (−/−) gene deleted and wild-type mice demonstrated the use of 94m Tc-MIBI to detect Pgp function. The reversal effect of a Pgp modulator was shown in tissue distribution studies of KB 3–1 (Pgp-) and KB 8–5 (Pgp+) tumor-bearing nude mice. The current 94m Tc-MIBI experiments parallel previous studies employing 99m Tc-MIBI, showing essentially identical performance of the two technetium radiotracers and providing biological validation of 94m Tc-MIBI for PET imaging of multidrug resistance. |
| format | Article |
| id | doaj-art-f73f8cbbafb24b7dae0e0012b3672f36 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-f73f8cbbafb24b7dae0e0012b3672f362025-01-02T22:37:02ZengSAGE PublishingMolecular Imaging1536-01212005-01-01410.1162/1535350020050416610.1162_15353500200504166Imaging Multidrug Resistance P-glycoprotein Transport Function Using MicroPET with Technetium-94m-SestamibiHeather M. BigottJulie L. PriorDavid R. Piwnica-WormsMichael J. WelchThe best characterized mechanism of multidrug resistance (MDR) in cancer involves the MDR1 efflux transporter P-glycoprotein (Pgp). The positron-emitting radiotracer hexakis (2-methoxyisobutylisonitrile)- 94m Tc ( 94m Tc-MIBI) was synthesized and validated in cell transport studies as a substrate for MDR1 Pgp. In vivo small-scale PET imaging and biodistribution studies of mdr1a/1b (−/−) gene deleted and wild-type mice demonstrated the use of 94m Tc-MIBI to detect Pgp function. The reversal effect of a Pgp modulator was shown in tissue distribution studies of KB 3–1 (Pgp-) and KB 8–5 (Pgp+) tumor-bearing nude mice. The current 94m Tc-MIBI experiments parallel previous studies employing 99m Tc-MIBI, showing essentially identical performance of the two technetium radiotracers and providing biological validation of 94m Tc-MIBI for PET imaging of multidrug resistance.https://doi.org/10.1162/15353500200504166 |
| spellingShingle | Heather M. Bigott Julie L. Prior David R. Piwnica-Worms Michael J. Welch Imaging Multidrug Resistance P-glycoprotein Transport Function Using MicroPET with Technetium-94m-Sestamibi Molecular Imaging |
| title | Imaging Multidrug Resistance P-glycoprotein Transport Function Using MicroPET with Technetium-94m-Sestamibi |
| title_full | Imaging Multidrug Resistance P-glycoprotein Transport Function Using MicroPET with Technetium-94m-Sestamibi |
| title_fullStr | Imaging Multidrug Resistance P-glycoprotein Transport Function Using MicroPET with Technetium-94m-Sestamibi |
| title_full_unstemmed | Imaging Multidrug Resistance P-glycoprotein Transport Function Using MicroPET with Technetium-94m-Sestamibi |
| title_short | Imaging Multidrug Resistance P-glycoprotein Transport Function Using MicroPET with Technetium-94m-Sestamibi |
| title_sort | imaging multidrug resistance p glycoprotein transport function using micropet with technetium 94m sestamibi |
| url | https://doi.org/10.1162/15353500200504166 |
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