Structural and antimicrobial properties of synthesized gold nanoparticles using biological and chemical approaches
This study explores the synthesis and characterization of gold nanoparticles (AuNPs) using green and chemical methods, employing ginger extract and curcumin as reducing agents, in comparison to sodium citrate reduction. The biosynthesized AuNPs synthesized with ginger extract exhibited an average hy...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2024.1482102/full |
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| author | Hamidreza Kalantari Hamidreza Kalantari Raymond J. Turner |
| author_facet | Hamidreza Kalantari Hamidreza Kalantari Raymond J. Turner |
| author_sort | Hamidreza Kalantari |
| collection | DOAJ |
| description | This study explores the synthesis and characterization of gold nanoparticles (AuNPs) using green and chemical methods, employing ginger extract and curcumin as reducing agents, in comparison to sodium citrate reduction. The biosynthesized AuNPs synthesized with ginger extract exhibited an average hydrodynamic diameter of 15 and 10 nm for curcumin-conjugated AuNPs, while chemically synthesized AuNPs with sodium citrate displayed an average size of 10 nm. Assessments via Zeta potential measurements revealed negative surface charges across all samples, with the curcumin-conjugated AuNPs showing −36.3 mV, ginger extract-synthesized AuNPs showing −31.7 mV, and chemically produced gold nanoparticles having a surface charge of −40.4 mV. Transmission Electron Microscopy (TEM) confirmed spherical morphologies for the synthesized nanoparticles,and it revealed the presence of biomolecules embedded within the nanoparticles synthesized using biological materials, whereas chemically synthesized AuNPs lacked such features. The FTIR spectra of the biosynthesized AuNPs highlighted the presence of phenolic and aromatic compounds from the ginger extract and curcumin, indicating their role in coating the nanoparticles. Gas chromatography-mass spectrometry (GC-MS) analysis identified gingerol as a key component in the ginger extract, contributing to nanoparticle capping. The antimicrobial efficacy of the AuNPs was evaluated against P. aeruginosa, E. coli, and S. aureus, revealing superior activity for curcumin-AuNPs, with ginger-AuNPs also outperforming chemically synthesized counterparts. These findings confirm the advantages of biological approaches, using a plant extract like ginger and pure curcumin suspension, for better size distribution when used as reducing agents, along with improved antimicrobial efficacy compared to chemically produced gold nanoparticles synthesized with sodium citrate. This study also highlight the potential of green-synthesized AuNPs in biomedical applications, due to their enhanced stability from higher surface charge and the repeatability of biological methods. |
| format | Article |
| id | doaj-art-f70a964fb85148fcaa9ecf59c647405c |
| institution | Kabale University |
| issn | 2296-2646 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj-art-f70a964fb85148fcaa9ecf59c647405c2024-11-13T04:53:57ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462024-11-011210.3389/fchem.2024.14821021482102Structural and antimicrobial properties of synthesized gold nanoparticles using biological and chemical approachesHamidreza Kalantari0Hamidreza Kalantari1Raymond J. Turner2Department of Biological Sciences, Microbial Biochemistry Laboratory, University of Calgary, Calgary, NW, CanadaDepartment of Microbiology, Islamic Azad University, Tehran, IranDepartment of Biological Sciences, Microbial Biochemistry Laboratory, University of Calgary, Calgary, NW, CanadaThis study explores the synthesis and characterization of gold nanoparticles (AuNPs) using green and chemical methods, employing ginger extract and curcumin as reducing agents, in comparison to sodium citrate reduction. The biosynthesized AuNPs synthesized with ginger extract exhibited an average hydrodynamic diameter of 15 and 10 nm for curcumin-conjugated AuNPs, while chemically synthesized AuNPs with sodium citrate displayed an average size of 10 nm. Assessments via Zeta potential measurements revealed negative surface charges across all samples, with the curcumin-conjugated AuNPs showing −36.3 mV, ginger extract-synthesized AuNPs showing −31.7 mV, and chemically produced gold nanoparticles having a surface charge of −40.4 mV. Transmission Electron Microscopy (TEM) confirmed spherical morphologies for the synthesized nanoparticles,and it revealed the presence of biomolecules embedded within the nanoparticles synthesized using biological materials, whereas chemically synthesized AuNPs lacked such features. The FTIR spectra of the biosynthesized AuNPs highlighted the presence of phenolic and aromatic compounds from the ginger extract and curcumin, indicating their role in coating the nanoparticles. Gas chromatography-mass spectrometry (GC-MS) analysis identified gingerol as a key component in the ginger extract, contributing to nanoparticle capping. The antimicrobial efficacy of the AuNPs was evaluated against P. aeruginosa, E. coli, and S. aureus, revealing superior activity for curcumin-AuNPs, with ginger-AuNPs also outperforming chemically synthesized counterparts. These findings confirm the advantages of biological approaches, using a plant extract like ginger and pure curcumin suspension, for better size distribution when used as reducing agents, along with improved antimicrobial efficacy compared to chemically produced gold nanoparticles synthesized with sodium citrate. This study also highlight the potential of green-synthesized AuNPs in biomedical applications, due to their enhanced stability from higher surface charge and the repeatability of biological methods.https://www.frontiersin.org/articles/10.3389/fchem.2024.1482102/fullgold nanoparticles (AuNPs)green synthesisginger AuNPscurcumin AuNPschemical AuNPs (sodium citrate)antimicrobial activity |
| spellingShingle | Hamidreza Kalantari Hamidreza Kalantari Raymond J. Turner Structural and antimicrobial properties of synthesized gold nanoparticles using biological and chemical approaches Frontiers in Chemistry gold nanoparticles (AuNPs) green synthesis ginger AuNPs curcumin AuNPs chemical AuNPs (sodium citrate) antimicrobial activity |
| title | Structural and antimicrobial properties of synthesized gold nanoparticles using biological and chemical approaches |
| title_full | Structural and antimicrobial properties of synthesized gold nanoparticles using biological and chemical approaches |
| title_fullStr | Structural and antimicrobial properties of synthesized gold nanoparticles using biological and chemical approaches |
| title_full_unstemmed | Structural and antimicrobial properties of synthesized gold nanoparticles using biological and chemical approaches |
| title_short | Structural and antimicrobial properties of synthesized gold nanoparticles using biological and chemical approaches |
| title_sort | structural and antimicrobial properties of synthesized gold nanoparticles using biological and chemical approaches |
| topic | gold nanoparticles (AuNPs) green synthesis ginger AuNPs curcumin AuNPs chemical AuNPs (sodium citrate) antimicrobial activity |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2024.1482102/full |
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