Improving small-scale cultivation of Spodoptera frugiperda 9 cells by silanizing glassware
Abstract Cultivating insect cells in glass vessels can be challenging. Due to uncontrolled cell adhesion and associated cell loss as well as clumping, the replication of experiments is put at risk. A cost-effective solution to improve and stabilize cultivation may be to silanize glass vessels, makin...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-12-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-84093-w |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559556387241984 |
|---|---|
| author | Kristina Worch Benjamin Ole Mühlnickel Jana Pieper Antje Burse |
| author_facet | Kristina Worch Benjamin Ole Mühlnickel Jana Pieper Antje Burse |
| author_sort | Kristina Worch |
| collection | DOAJ |
| description | Abstract Cultivating insect cells in glass vessels can be challenging. Due to uncontrolled cell adhesion and associated cell loss as well as clumping, the replication of experiments is put at risk. A cost-effective solution to improve and stabilize cultivation may be to silanize glass vessels, making them more hydrophobic and chemically inert. Therefore, the cell growth parameters of Spodoptera frugiperda (Sf) 9 cells were characterized in an experiment comparing silanized and non-silanized vessels of three different sizes. Silanization had a significantly positive effect on living cell count and viability in small-scale cultivation (10 ml culture tubes and 50 ml culture flasks) by reducing adhesion of cells to the vessel walls. The treatment also improved the comparability of the biological replicates by reducing the variance of measurements. No such effects could be observed for larger 250 ml culture flasks. Overall, the results indicate that silanization can improve cultivation, especially when working with small glass vessels. |
| format | Article |
| id | doaj-art-f559d7b5db1541a08ba6bdae9853a1b9 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-f559d7b5db1541a08ba6bdae9853a1b92025-01-05T12:25:13ZengNature PortfolioScientific Reports2045-23222024-12-011411410.1038/s41598-024-84093-wImproving small-scale cultivation of Spodoptera frugiperda 9 cells by silanizing glasswareKristina Worch0Benjamin Ole Mühlnickel1Jana Pieper2Antje Burse3Department of Medical Engineering and Biotechnology, Ernst-Abbe-Hochschule, University of Applied SciencesDepartment of Medical Engineering and Biotechnology, Ernst-Abbe-Hochschule, University of Applied SciencesDepartment of Medical Engineering and Biotechnology, Ernst-Abbe-Hochschule, University of Applied SciencesDepartment of Medical Engineering and Biotechnology, Ernst-Abbe-Hochschule, University of Applied SciencesAbstract Cultivating insect cells in glass vessels can be challenging. Due to uncontrolled cell adhesion and associated cell loss as well as clumping, the replication of experiments is put at risk. A cost-effective solution to improve and stabilize cultivation may be to silanize glass vessels, making them more hydrophobic and chemically inert. Therefore, the cell growth parameters of Spodoptera frugiperda (Sf) 9 cells were characterized in an experiment comparing silanized and non-silanized vessels of three different sizes. Silanization had a significantly positive effect on living cell count and viability in small-scale cultivation (10 ml culture tubes and 50 ml culture flasks) by reducing adhesion of cells to the vessel walls. The treatment also improved the comparability of the biological replicates by reducing the variance of measurements. No such effects could be observed for larger 250 ml culture flasks. Overall, the results indicate that silanization can improve cultivation, especially when working with small glass vessels.https://doi.org/10.1038/s41598-024-84093-wCell cultureSf9SilanizationReproducibility |
| spellingShingle | Kristina Worch Benjamin Ole Mühlnickel Jana Pieper Antje Burse Improving small-scale cultivation of Spodoptera frugiperda 9 cells by silanizing glassware Scientific Reports Cell culture Sf9 Silanization Reproducibility |
| title | Improving small-scale cultivation of Spodoptera frugiperda 9 cells by silanizing glassware |
| title_full | Improving small-scale cultivation of Spodoptera frugiperda 9 cells by silanizing glassware |
| title_fullStr | Improving small-scale cultivation of Spodoptera frugiperda 9 cells by silanizing glassware |
| title_full_unstemmed | Improving small-scale cultivation of Spodoptera frugiperda 9 cells by silanizing glassware |
| title_short | Improving small-scale cultivation of Spodoptera frugiperda 9 cells by silanizing glassware |
| title_sort | improving small scale cultivation of spodoptera frugiperda 9 cells by silanizing glassware |
| topic | Cell culture Sf9 Silanization Reproducibility |
| url | https://doi.org/10.1038/s41598-024-84093-w |
| work_keys_str_mv | AT kristinaworch improvingsmallscalecultivationofspodopterafrugiperda9cellsbysilanizingglassware AT benjaminolemuhlnickel improvingsmallscalecultivationofspodopterafrugiperda9cellsbysilanizingglassware AT janapieper improvingsmallscalecultivationofspodopterafrugiperda9cellsbysilanizingglassware AT antjeburse improvingsmallscalecultivationofspodopterafrugiperda9cellsbysilanizingglassware |