AozC, a zn(II)2Cys6 transcription factor, negatively regulates salt tolerance in Aspergillus oryzae by controlling fatty acid biosynthesis
Abstract Background In the soy sauce fermentation industry, Aspergillus oryzae (A. oryzae) plays an essential role and is frequently subjected to high salinity levels, which pose a significant osmotic stress. This environmental challenge necessitates the activation of stress response mechanisms with...
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2025-01-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-024-02639-z |
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| author | Wenbin Yu Zeying Zhao Yufei Zhang Yayi Tu Bin He |
| author_facet | Wenbin Yu Zeying Zhao Yufei Zhang Yayi Tu Bin He |
| author_sort | Wenbin Yu |
| collection | DOAJ |
| description | Abstract Background In the soy sauce fermentation industry, Aspergillus oryzae (A. oryzae) plays an essential role and is frequently subjected to high salinity levels, which pose a significant osmotic stress. This environmental challenge necessitates the activation of stress response mechanisms within the fungus. The Zn(II)2Cys6 family of transcription factors, known for their zinc binuclear cluster-containing proteins, are key regulators in fungi, modulating various cellular functions such as stress adaptation and metabolic pathways. Results Overexpression of AozC decreased growth rates in the presence of salt, while its knockdown enhanced growth, the number of spores, and biomass, particularly under conditions of 15% salt concentration, doubling these metrics compared to the wild type. Conversely, the knockdown of AozC via RNA interference significantly enhanced spore density and dry biomass, particularly under 15% salt stress, where these parameters were markedly improved over the wild type strain. Moreover, the overexpression of AozC led to a downregulation of the FAD2 gene, a pivotal enzyme in the biosynthesis of unsaturated fatty acids (UFAs), which are essential for preserving cell membrane fluidity and integrity under saline conditions. Transcriptome profiling further exposed the influence of AozC on the regulation of UFA biosynthesis and the modulation of critical stress response pathways. Notably, the regulatory role of AozC in the mitogen-activated protein kinase (MAPK) signaling and ABC transporters pathways was highlighted, underscoring its significance in cellular osmotic balance and endoplasmic reticulum homeostasis. These findings collectively indicate that AozC functions as a negative regulator of salt tolerance in A. oryzae. Conclusion This research suggest that AozC acts as a negative regulator in salt tolerance and modulates fatty acid biosynthesis in response to osmotic stress. These results provide insights into the regulatory mechanisms of stress adaptation in A. oryzae. |
| format | Article |
| id | doaj-art-f4600740aafa467685ded91f9c966b6d |
| institution | Kabale University |
| issn | 1475-2859 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj-art-f4600740aafa467685ded91f9c966b6d2025-01-12T12:45:37ZengBMCMicrobial Cell Factories1475-28592025-01-0124111310.1186/s12934-024-02639-zAozC, a zn(II)2Cys6 transcription factor, negatively regulates salt tolerance in Aspergillus oryzae by controlling fatty acid biosynthesisWenbin Yu0Zeying Zhao1Yufei Zhang2Yayi Tu3Bin He4Key Laboratory of Natural Microbial Medicine Research of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal UniversityKey Laboratory of Natural Microbial Medicine Research of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal UniversityKey Laboratory of Natural Microbial Medicine Research of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal UniversityKey Laboratory of Natural Microbial Medicine Research of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal UniversityKey Laboratory of Natural Microbial Medicine Research of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal UniversityAbstract Background In the soy sauce fermentation industry, Aspergillus oryzae (A. oryzae) plays an essential role and is frequently subjected to high salinity levels, which pose a significant osmotic stress. This environmental challenge necessitates the activation of stress response mechanisms within the fungus. The Zn(II)2Cys6 family of transcription factors, known for their zinc binuclear cluster-containing proteins, are key regulators in fungi, modulating various cellular functions such as stress adaptation and metabolic pathways. Results Overexpression of AozC decreased growth rates in the presence of salt, while its knockdown enhanced growth, the number of spores, and biomass, particularly under conditions of 15% salt concentration, doubling these metrics compared to the wild type. Conversely, the knockdown of AozC via RNA interference significantly enhanced spore density and dry biomass, particularly under 15% salt stress, where these parameters were markedly improved over the wild type strain. Moreover, the overexpression of AozC led to a downregulation of the FAD2 gene, a pivotal enzyme in the biosynthesis of unsaturated fatty acids (UFAs), which are essential for preserving cell membrane fluidity and integrity under saline conditions. Transcriptome profiling further exposed the influence of AozC on the regulation of UFA biosynthesis and the modulation of critical stress response pathways. Notably, the regulatory role of AozC in the mitogen-activated protein kinase (MAPK) signaling and ABC transporters pathways was highlighted, underscoring its significance in cellular osmotic balance and endoplasmic reticulum homeostasis. These findings collectively indicate that AozC functions as a negative regulator of salt tolerance in A. oryzae. Conclusion This research suggest that AozC acts as a negative regulator in salt tolerance and modulates fatty acid biosynthesis in response to osmotic stress. These results provide insights into the regulatory mechanisms of stress adaptation in A. oryzae.https://doi.org/10.1186/s12934-024-02639-zAspergillus oryzaeDifferentially expressed genesSalt treatmentFatty acidTranscriptomeTranscription factor |
| spellingShingle | Wenbin Yu Zeying Zhao Yufei Zhang Yayi Tu Bin He AozC, a zn(II)2Cys6 transcription factor, negatively regulates salt tolerance in Aspergillus oryzae by controlling fatty acid biosynthesis Microbial Cell Factories Aspergillus oryzae Differentially expressed genes Salt treatment Fatty acid Transcriptome Transcription factor |
| title | AozC, a zn(II)2Cys6 transcription factor, negatively regulates salt tolerance in Aspergillus oryzae by controlling fatty acid biosynthesis |
| title_full | AozC, a zn(II)2Cys6 transcription factor, negatively regulates salt tolerance in Aspergillus oryzae by controlling fatty acid biosynthesis |
| title_fullStr | AozC, a zn(II)2Cys6 transcription factor, negatively regulates salt tolerance in Aspergillus oryzae by controlling fatty acid biosynthesis |
| title_full_unstemmed | AozC, a zn(II)2Cys6 transcription factor, negatively regulates salt tolerance in Aspergillus oryzae by controlling fatty acid biosynthesis |
| title_short | AozC, a zn(II)2Cys6 transcription factor, negatively regulates salt tolerance in Aspergillus oryzae by controlling fatty acid biosynthesis |
| title_sort | aozc a zn ii 2cys6 transcription factor negatively regulates salt tolerance in aspergillus oryzae by controlling fatty acid biosynthesis |
| topic | Aspergillus oryzae Differentially expressed genes Salt treatment Fatty acid Transcriptome Transcription factor |
| url | https://doi.org/10.1186/s12934-024-02639-z |
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