Valorization of local regional apple (Malus domestica Borkh.) cultivars versus commercial samples from Spain: Phenolic compounds by HPLC-MS/MS, cytotoxicity and biological potential on nitric oxide radicals and lipoxygenase inhibition
Sustainability in the fruit industry and protection of local cultivars are nowadays important tasks for consumers and authorities. Phenolic compounds are interesting to explore because they play an important role in fruit-related health benefits. The phenolic profile of the regional and commercial S...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624006340 |
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| author | Adrián Millán-Laleona Pilar Cebollada Giovanni Caprioli Diletta Piatti Filippo Maggi Ana Pina Carlota Gómez-Rincón Víctor López |
| author_facet | Adrián Millán-Laleona Pilar Cebollada Giovanni Caprioli Diletta Piatti Filippo Maggi Ana Pina Carlota Gómez-Rincón Víctor López |
| author_sort | Adrián Millán-Laleona |
| collection | DOAJ |
| description | Sustainability in the fruit industry and protection of local cultivars are nowadays important tasks for consumers and authorities. Phenolic compounds are interesting to explore because they play an important role in fruit-related health benefits. The phenolic profile of the regional and commercial Spanish apple cultivars was performed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). For a deeper understanding of their anti-inflammatory and antioxidant potential, the 5-lipoxigenase inhibition and NO− scavenging were evaluated. Moreover, cellular viability was determined to perform MTT assay in HepG2 and HeLa cell lines to discard cytotoxicity. The results showed that procyanidin B2 (PB2), 3-caffeoylquinic acid, epicatechin and 4-hydroxy benzoic acid were the most abundant phenolic compounds. Autochthonous apple cultivars showed better 5-LOX/NO− inhibition and nontoxic effects in the tested cell lines with an interesting profile of phenolic compounds. Local autochthonous apples can be considered a better source of bioactive compounds than the commercial samples. |
| format | Article |
| id | doaj-art-f32aa3b0f3e64e2c9b73a3ff7b336a83 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-f32aa3b0f3e64e2c9b73a3ff7b336a832025-01-12T05:24:43ZengElsevierJournal of Functional Foods1756-46462025-01-01124106631Valorization of local regional apple (Malus domestica Borkh.) cultivars versus commercial samples from Spain: Phenolic compounds by HPLC-MS/MS, cytotoxicity and biological potential on nitric oxide radicals and lipoxygenase inhibitionAdrián Millán-Laleona0Pilar Cebollada1Giovanni Caprioli2Diletta Piatti3Filippo Maggi4Ana Pina5Carlota Gómez-Rincón6Víctor López7Department of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Villanueva de Gállego (Zaragoza), Spain; Instituto Agroalimentario de Aragón-IA2, CITA-Universidad de Zaragoza, 50013 Zaragoza, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Villanueva de Gállego (Zaragoza), SpainChemistry Interdisciplinary Project (ChIP), School of Pharmacy, University of Camerino, Via Madonna delle Carceri, 62032 Camerino, ItalyChemistry Interdisciplinary Project (ChIP), School of Pharmacy, University of Camerino, Via Madonna delle Carceri, 62032 Camerino, ItalyChemistry Interdisciplinary Project (ChIP), School of Pharmacy, University of Camerino, Via Madonna delle Carceri, 62032 Camerino, ItalyInstituto Agroalimentario de Aragón-IA2, CITA-Universidad de Zaragoza, 50013 Zaragoza, Spain; Departamento de Ciencia Vegetal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Zaragoza, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Villanueva de Gállego (Zaragoza), Spain; Instituto Agroalimentario de Aragón-IA2, CITA-Universidad de Zaragoza, 50013 Zaragoza, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Villanueva de Gállego (Zaragoza), Spain; Instituto Agroalimentario de Aragón-IA2, CITA-Universidad de Zaragoza, 50013 Zaragoza, Spain; Corresponding author at: Department of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Zaragoza, Spain.Sustainability in the fruit industry and protection of local cultivars are nowadays important tasks for consumers and authorities. Phenolic compounds are interesting to explore because they play an important role in fruit-related health benefits. The phenolic profile of the regional and commercial Spanish apple cultivars was performed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). For a deeper understanding of their anti-inflammatory and antioxidant potential, the 5-lipoxigenase inhibition and NO− scavenging were evaluated. Moreover, cellular viability was determined to perform MTT assay in HepG2 and HeLa cell lines to discard cytotoxicity. The results showed that procyanidin B2 (PB2), 3-caffeoylquinic acid, epicatechin and 4-hydroxy benzoic acid were the most abundant phenolic compounds. Autochthonous apple cultivars showed better 5-LOX/NO− inhibition and nontoxic effects in the tested cell lines with an interesting profile of phenolic compounds. Local autochthonous apples can be considered a better source of bioactive compounds than the commercial samples.http://www.sciencedirect.com/science/article/pii/S1756464624006340FruitsApplePolyphenolsFlavonoidsHPLC-MS/MSAnti-inflammatory |
| spellingShingle | Adrián Millán-Laleona Pilar Cebollada Giovanni Caprioli Diletta Piatti Filippo Maggi Ana Pina Carlota Gómez-Rincón Víctor López Valorization of local regional apple (Malus domestica Borkh.) cultivars versus commercial samples from Spain: Phenolic compounds by HPLC-MS/MS, cytotoxicity and biological potential on nitric oxide radicals and lipoxygenase inhibition Journal of Functional Foods Fruits Apple Polyphenols Flavonoids HPLC-MS/MS Anti-inflammatory |
| title | Valorization of local regional apple (Malus domestica Borkh.) cultivars versus commercial samples from Spain: Phenolic compounds by HPLC-MS/MS, cytotoxicity and biological potential on nitric oxide radicals and lipoxygenase inhibition |
| title_full | Valorization of local regional apple (Malus domestica Borkh.) cultivars versus commercial samples from Spain: Phenolic compounds by HPLC-MS/MS, cytotoxicity and biological potential on nitric oxide radicals and lipoxygenase inhibition |
| title_fullStr | Valorization of local regional apple (Malus domestica Borkh.) cultivars versus commercial samples from Spain: Phenolic compounds by HPLC-MS/MS, cytotoxicity and biological potential on nitric oxide radicals and lipoxygenase inhibition |
| title_full_unstemmed | Valorization of local regional apple (Malus domestica Borkh.) cultivars versus commercial samples from Spain: Phenolic compounds by HPLC-MS/MS, cytotoxicity and biological potential on nitric oxide radicals and lipoxygenase inhibition |
| title_short | Valorization of local regional apple (Malus domestica Borkh.) cultivars versus commercial samples from Spain: Phenolic compounds by HPLC-MS/MS, cytotoxicity and biological potential on nitric oxide radicals and lipoxygenase inhibition |
| title_sort | valorization of local regional apple malus domestica borkh cultivars versus commercial samples from spain phenolic compounds by hplc ms ms cytotoxicity and biological potential on nitric oxide radicals and lipoxygenase inhibition |
| topic | Fruits Apple Polyphenols Flavonoids HPLC-MS/MS Anti-inflammatory |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624006340 |
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