Saline pasture improve meat quality in Qinghai Tibetan sheep through changes in the rumen microbiota
IntroductionThis study aimed to investigate the effects of natural forage from different regions (saline-alkali and non-saline-alkali areas) on the rumen microbiota, muscle metabolites, and meat quality of Tibetan sheep.MethodsTargeted and non-targeted metabolomics were used to comprehensively analy...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1573040/full |
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| author | Yujiao Jia Lijuan Han Shengzhen Hou Linsheng Gui Zhenzhen Yuan Shengnan Sun Zhiyou Wang Baochun Yang Chao Yang |
| author_facet | Yujiao Jia Lijuan Han Shengzhen Hou Linsheng Gui Zhenzhen Yuan Shengnan Sun Zhiyou Wang Baochun Yang Chao Yang |
| author_sort | Yujiao Jia |
| collection | DOAJ |
| description | IntroductionThis study aimed to investigate the effects of natural forage from different regions (saline-alkali and non-saline-alkali areas) on the rumen microbiota, muscle metabolites, and meat quality of Tibetan sheep.MethodsTargeted and non-targeted metabolomics were used to comprehensively analyze both pasture and meat quality, metabolites. Additionally, 16S rDNA sequencing was employed to analyze the rumen microbial community structure of Tibetan sheep.ResultsThe results showed that the natural saline-alkali forage (HG group) had higher protein content, lower fiber content, higher relative feed value, and better quality. Metabolomic analysis revealed significant accumulation of flavonoids and upregulation of amino acid metabolism in the HG group. Additionally, the natural saline-alkali forage significantly increased amino acid deposition in Tibetan sheep muscle, markedly enhanced the redness value (a*), and significantly reduced the yellowness value (b*). Furthermore, the natural saline-alkali forage altered the rumen fermentation patterns in Tibetan sheep, leading to a significant increase in the abundance of F082 and WCHB1-41, while significantly reducing the abundance of Prevotellaceae_UCG-003. Correlation analysis revealed that these microbial taxa were significantly influenced by the natural saline-alkali forage,while also showing significant associations with muscle quality parameters (a*, b*) and metabolites (cysteine, C18:1n9, etc.).DiscussionOverall, the natural saline-alkali forage demonstrated superior quality and metabolite content compared to natural non-saline-alkali forage. Furthermore, this saline-alkali forage significantly influenced the abundance of specific rumen microbiota in Tibetan sheep, consequently regulating muscle coloration and amino acid deposition. |
| format | Article |
| id | doaj-art-f1a409b2d7be4ca08d0dda363612b5d9 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-f1a409b2d7be4ca08d0dda363612b5d92025-08-20T03:44:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-08-011610.3389/fmicb.2025.15730401573040Saline pasture improve meat quality in Qinghai Tibetan sheep through changes in the rumen microbiotaYujiao JiaLijuan HanShengzhen HouLinsheng GuiZhenzhen YuanShengnan SunZhiyou WangBaochun YangChao YangIntroductionThis study aimed to investigate the effects of natural forage from different regions (saline-alkali and non-saline-alkali areas) on the rumen microbiota, muscle metabolites, and meat quality of Tibetan sheep.MethodsTargeted and non-targeted metabolomics were used to comprehensively analyze both pasture and meat quality, metabolites. Additionally, 16S rDNA sequencing was employed to analyze the rumen microbial community structure of Tibetan sheep.ResultsThe results showed that the natural saline-alkali forage (HG group) had higher protein content, lower fiber content, higher relative feed value, and better quality. Metabolomic analysis revealed significant accumulation of flavonoids and upregulation of amino acid metabolism in the HG group. Additionally, the natural saline-alkali forage significantly increased amino acid deposition in Tibetan sheep muscle, markedly enhanced the redness value (a*), and significantly reduced the yellowness value (b*). Furthermore, the natural saline-alkali forage altered the rumen fermentation patterns in Tibetan sheep, leading to a significant increase in the abundance of F082 and WCHB1-41, while significantly reducing the abundance of Prevotellaceae_UCG-003. Correlation analysis revealed that these microbial taxa were significantly influenced by the natural saline-alkali forage,while also showing significant associations with muscle quality parameters (a*, b*) and metabolites (cysteine, C18:1n9, etc.).DiscussionOverall, the natural saline-alkali forage demonstrated superior quality and metabolite content compared to natural non-saline-alkali forage. Furthermore, this saline-alkali forage significantly influenced the abundance of specific rumen microbiota in Tibetan sheep, consequently regulating muscle coloration and amino acid deposition.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1573040/fullTibetan sheepsaline-alkali stressforage qualitymeat qualitymetabolomicsrumen microorganism |
| spellingShingle | Yujiao Jia Lijuan Han Shengzhen Hou Linsheng Gui Zhenzhen Yuan Shengnan Sun Zhiyou Wang Baochun Yang Chao Yang Saline pasture improve meat quality in Qinghai Tibetan sheep through changes in the rumen microbiota Frontiers in Microbiology Tibetan sheep saline-alkali stress forage quality meat quality metabolomics rumen microorganism |
| title | Saline pasture improve meat quality in Qinghai Tibetan sheep through changes in the rumen microbiota |
| title_full | Saline pasture improve meat quality in Qinghai Tibetan sheep through changes in the rumen microbiota |
| title_fullStr | Saline pasture improve meat quality in Qinghai Tibetan sheep through changes in the rumen microbiota |
| title_full_unstemmed | Saline pasture improve meat quality in Qinghai Tibetan sheep through changes in the rumen microbiota |
| title_short | Saline pasture improve meat quality in Qinghai Tibetan sheep through changes in the rumen microbiota |
| title_sort | saline pasture improve meat quality in qinghai tibetan sheep through changes in the rumen microbiota |
| topic | Tibetan sheep saline-alkali stress forage quality meat quality metabolomics rumen microorganism |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1573040/full |
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