Miniaturized scalable arrayed CRISPR screening in primary cells enables discovery at the single donor resolution

Miniaturized scalable arrayed CRISPR screening in primary cells enables discovery at the single donor resolution

Abstract High-efficiency gene editing in primary human cells is critical for advancing therapeutic development and functional genomics, yet conventional electroporation platforms often require high cell input and are poorly suited to parallelized experiments. Here we introduce a next-generation digi...

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Main Authors: Miti A. Patel, Brittany P. Boribong, Hugo Sinha, Bin Xiao, Keqiang Xie, Philippe Q. N. Vo, Andrew B. Chin, Ayoub Ellouzi, Samuel R. Little, Steve Shih, Hao Wu, William J. Muller, Alison Hirukawa
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-025-13532-z
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https://doi.org/10.1038/s41598-025-13532-z

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