The role of Ca2+ signalling and InsP3R in the pathogenesis of intrahepatic cholestasis of pregnancy
Background In order to contribute new insights for future prevention and treatment of intrahepatic cholestasis of pregnancy (ICP), and to promote positive pregnancy outcomes, we evaluated serum Ca2+ levels and inositol 1,4,5-trisphosphate receptor (InsP3R) expression in the liver tissue of a rat ICP...
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| Format: | Article |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Obstetrics and Gynaecology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/01443615.2024.2345276 |
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| author | Dan Pan Mengting Jiang Guoxian Tao Jinmei Shi Zhiwei Song Ren Chen Dongguo Wang |
| author_facet | Dan Pan Mengting Jiang Guoxian Tao Jinmei Shi Zhiwei Song Ren Chen Dongguo Wang |
| author_sort | Dan Pan |
| collection | DOAJ |
| description | Background In order to contribute new insights for future prevention and treatment of intrahepatic cholestasis of pregnancy (ICP), and to promote positive pregnancy outcomes, we evaluated serum Ca2+ levels and inositol 1,4,5-trisphosphate receptor (InsP3R) expression in the liver tissue of a rat ICP model.Methods After establishing the model by injection of oestradiol benzoate and progesterone into pregnant rats, animals were divided into normal control (n = 5) and ICP model groups (n = 5). The expression of InsP3R protein in the liver, and serum levels of Ca2+, glycocholic acid and bile acid were detected.Results InsP3R mRNA and protein were significantly lower in the ICP model group compared to the normal group, as determined by qPCR and immunohistochemistry, respectively. Serum enzyme-linked immunosorbent assay results revealed significantly higher levels of glycocholic acid and bile acid in the ICP model group compared to the normal group, while Ca2+ levels were significantly lower. The levers of Ca2+ were significantly and negatively correlated with the levels of glycocholic acid. The observed decrease in Ca2+ was associated with an increase in total bile acids, but there was no significant correlation.Conclusions Our results revealed that the expression of InsP3R and serum Ca2+ levels was significantly decreased in the liver tissue of ICP model rats. Additionally, Ca2+ levels were found to be negatively correlated with the level of glycocholic acid. |
| format | Article |
| id | doaj-art-ef74086da1834d30932ed16e43788961 |
| institution | Kabale University |
| issn | 0144-3615 1364-6893 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Obstetrics and Gynaecology |
| spelling | doaj-art-ef74086da1834d30932ed16e437889612025-01-09T12:13:18ZengTaylor & Francis GroupJournal of Obstetrics and Gynaecology0144-36151364-68932024-12-0144110.1080/01443615.2024.2345276The role of Ca2+ signalling and InsP3R in the pathogenesis of intrahepatic cholestasis of pregnancyDan Pan0Mengting Jiang1Guoxian Tao2Jinmei Shi3Zhiwei Song4Ren Chen5Dongguo Wang6Department of Obstetrics and Gynecology, Taizhou Municipal Hospital affiliated with Taizhou University, Taizhou, ChinaDepartment of Obstetrics and Gynecology, Taizhou Municipal Hospital affiliated with Taizhou University, Taizhou, ChinaDepartment of Obstetrics and Gynecology, Taizhou Municipal Hospital affiliated with Taizhou University, Taizhou, ChinaDepartment of Obstetrics and Gynecology, Taizhou Municipal Hospital affiliated with Taizhou University, Taizhou, ChinaDepartment of Medical Laboratory, Taizhou Municipal Hospital affiliated with Taizhou University, Taizhou, ChinaDepartment of Obstetrics and Gynecology, Taizhou Municipal Hospital affiliated with Taizhou University, Taizhou, ChinaDepartment of Central Laboratory, Taizhou Municipal Hospital affiliated with Taizhou University, Taizhou, ChinaBackground In order to contribute new insights for future prevention and treatment of intrahepatic cholestasis of pregnancy (ICP), and to promote positive pregnancy outcomes, we evaluated serum Ca2+ levels and inositol 1,4,5-trisphosphate receptor (InsP3R) expression in the liver tissue of a rat ICP model.Methods After establishing the model by injection of oestradiol benzoate and progesterone into pregnant rats, animals were divided into normal control (n = 5) and ICP model groups (n = 5). The expression of InsP3R protein in the liver, and serum levels of Ca2+, glycocholic acid and bile acid were detected.Results InsP3R mRNA and protein were significantly lower in the ICP model group compared to the normal group, as determined by qPCR and immunohistochemistry, respectively. Serum enzyme-linked immunosorbent assay results revealed significantly higher levels of glycocholic acid and bile acid in the ICP model group compared to the normal group, while Ca2+ levels were significantly lower. The levers of Ca2+ were significantly and negatively correlated with the levels of glycocholic acid. The observed decrease in Ca2+ was associated with an increase in total bile acids, but there was no significant correlation.Conclusions Our results revealed that the expression of InsP3R and serum Ca2+ levels was significantly decreased in the liver tissue of ICP model rats. Additionally, Ca2+ levels were found to be negatively correlated with the level of glycocholic acid.https://www.tandfonline.com/doi/10.1080/01443615.2024.2345276Intrahepatic cholestasis of pregnancyInsP3Rcalcium ion |
| spellingShingle | Dan Pan Mengting Jiang Guoxian Tao Jinmei Shi Zhiwei Song Ren Chen Dongguo Wang The role of Ca2+ signalling and InsP3R in the pathogenesis of intrahepatic cholestasis of pregnancy Journal of Obstetrics and Gynaecology Intrahepatic cholestasis of pregnancy InsP3R calcium ion |
| title | The role of Ca2+ signalling and InsP3R in the pathogenesis of intrahepatic cholestasis of pregnancy |
| title_full | The role of Ca2+ signalling and InsP3R in the pathogenesis of intrahepatic cholestasis of pregnancy |
| title_fullStr | The role of Ca2+ signalling and InsP3R in the pathogenesis of intrahepatic cholestasis of pregnancy |
| title_full_unstemmed | The role of Ca2+ signalling and InsP3R in the pathogenesis of intrahepatic cholestasis of pregnancy |
| title_short | The role of Ca2+ signalling and InsP3R in the pathogenesis of intrahepatic cholestasis of pregnancy |
| title_sort | role of ca2 signalling and insp3r in the pathogenesis of intrahepatic cholestasis of pregnancy |
| topic | Intrahepatic cholestasis of pregnancy InsP3R calcium ion |
| url | https://www.tandfonline.com/doi/10.1080/01443615.2024.2345276 |
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