Dose and time dependent cytotoxicity of Blepharis maderaspatensis (L) against various human cancer cell lines
Background: Traditionally, Blepharis maderaspatensis has been assigned antioxidant, anti-inflammatory, antimicrobial, and wound healing properties. Aim and Objectives: Present study was aimed to assess dose and time-dependent cytotoxic potential of B. maderaspatensis. Material and Methods: This was...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Al Ameen Medical College
2025-01-01
|
| Series: | Al Ameen Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://ajms.alameenmedical.org/ArticlePDFs/5%20AJMS%20V18.N1.2025%20p%2018-24.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841545869313179648 |
|---|---|
| author | Bhutkar Pratima Milind V. Suganthi Bhutkar Milind Vishnu |
| author_facet | Bhutkar Pratima Milind V. Suganthi Bhutkar Milind Vishnu |
| author_sort | Bhutkar Pratima Milind |
| collection | DOAJ |
| description | Background: Traditionally, Blepharis maderaspatensis has been assigned antioxidant, anti-inflammatory, antimicrobial, and wound healing properties. Aim and Objectives: Present study was aimed to assess dose and time-dependent cytotoxic potential of B. maderaspatensis. Material and Methods: This was an experimental study with positive and negative control under standard laboratory conditions. In vitro cytotoxic activity of ethanolic extract of B. maderaspatensis was assessed by MTT assay on MCF7, PA1, HT29, A375, HepG2 cancer cell lines. All the cell lines were treated with increasing concentration of the extract for 24, 48 and 72 hours or standard anticancer drug. IC50 value was calculated for each duration from sigmoid dose-response curve by using GraphPad Prism8 software. Results: Ethanolic extract of B. maderaspatensis was effective cytotoxic agent against all the tested cancer cell lines in dose and time-dependent manner. Cytotoxicity was maximum against A375 (melanoma) (IC50: 21.46μg/ml), followed by HT29 (colon cancer) cell lines (IC50: 32.66μg/ml). For all other cell lines IC50 values were >100μg/ml. Conclusions: Ethanolic extract of B. maderaspatensis is effective cytotoxic agent and can be evaluated further to study its mechanism of action. Investigations involving purification, identification of active principles and determination of mechanisms of action can be helpful in drug discovery program. |
| format | Article |
| id | doaj-art-ef72d7fcca0046b285dbcede0810ac52 |
| institution | Kabale University |
| issn | 0974-1143 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Al Ameen Medical College |
| record_format | Article |
| series | Al Ameen Journal of Medical Sciences |
| spelling | doaj-art-ef72d7fcca0046b285dbcede0810ac522025-01-11T12:11:37ZengAl Ameen Medical CollegeAl Ameen Journal of Medical Sciences0974-11432025-01-0118011824Dose and time dependent cytotoxicity of Blepharis maderaspatensis (L) against various human cancer cell linesBhutkar Pratima Milind0V. Suganthi1Bhutkar Milind Vishnu2Department of Physiology, Swamy Vivekanandha Medical College Hospital and Research Institute, Elayampalayam, Tiruchengode, Namakkal-637205, Tamil Nadu, IndiaDepartment of Physiology, Vinayaka Mission's , KV Medical College and Hospitals, NH47, Sankari Main Road Salem-636308, Tamil Nadu, IndiaDepartment of Physiology, Swamy Vivekanandha Medical College Hospital and Research Institute, Elayampalayam, Tiruchengode, Namakkal-637205, Tamil Nadu, IndiaBackground: Traditionally, Blepharis maderaspatensis has been assigned antioxidant, anti-inflammatory, antimicrobial, and wound healing properties. Aim and Objectives: Present study was aimed to assess dose and time-dependent cytotoxic potential of B. maderaspatensis. Material and Methods: This was an experimental study with positive and negative control under standard laboratory conditions. In vitro cytotoxic activity of ethanolic extract of B. maderaspatensis was assessed by MTT assay on MCF7, PA1, HT29, A375, HepG2 cancer cell lines. All the cell lines were treated with increasing concentration of the extract for 24, 48 and 72 hours or standard anticancer drug. IC50 value was calculated for each duration from sigmoid dose-response curve by using GraphPad Prism8 software. Results: Ethanolic extract of B. maderaspatensis was effective cytotoxic agent against all the tested cancer cell lines in dose and time-dependent manner. Cytotoxicity was maximum against A375 (melanoma) (IC50: 21.46μg/ml), followed by HT29 (colon cancer) cell lines (IC50: 32.66μg/ml). For all other cell lines IC50 values were >100μg/ml. Conclusions: Ethanolic extract of B. maderaspatensis is effective cytotoxic agent and can be evaluated further to study its mechanism of action. Investigations involving purification, identification of active principles and determination of mechanisms of action can be helpful in drug discovery program.https://ajms.alameenmedical.org/ArticlePDFs/5%20AJMS%20V18.N1.2025%20p%2018-24.pdfanticancermtt assaymelanomacolon cancer |
| spellingShingle | Bhutkar Pratima Milind V. Suganthi Bhutkar Milind Vishnu Dose and time dependent cytotoxicity of Blepharis maderaspatensis (L) against various human cancer cell lines Al Ameen Journal of Medical Sciences anticancer mtt assay melanoma colon cancer |
| title | Dose and time dependent cytotoxicity of Blepharis maderaspatensis (L) against various human cancer cell lines |
| title_full | Dose and time dependent cytotoxicity of Blepharis maderaspatensis (L) against various human cancer cell lines |
| title_fullStr | Dose and time dependent cytotoxicity of Blepharis maderaspatensis (L) against various human cancer cell lines |
| title_full_unstemmed | Dose and time dependent cytotoxicity of Blepharis maderaspatensis (L) against various human cancer cell lines |
| title_short | Dose and time dependent cytotoxicity of Blepharis maderaspatensis (L) against various human cancer cell lines |
| title_sort | dose and time dependent cytotoxicity of blepharis maderaspatensis l against various human cancer cell lines |
| topic | anticancer mtt assay melanoma colon cancer |
| url | https://ajms.alameenmedical.org/ArticlePDFs/5%20AJMS%20V18.N1.2025%20p%2018-24.pdf |
| work_keys_str_mv | AT bhutkarpratimamilind doseandtimedependentcytotoxicityofblepharismaderaspatensislagainstvarioushumancancercelllines AT vsuganthi doseandtimedependentcytotoxicityofblepharismaderaspatensislagainstvarioushumancancercelllines AT bhutkarmilindvishnu doseandtimedependentcytotoxicityofblepharismaderaspatensislagainstvarioushumancancercelllines |