Impact of cryopreservation on DNA damage in Acer platanoides L. seeds evaluated by the comet assay

Impact of cryopreservation on DNA damage in Acer platanoides L. seeds evaluated by the comet assay

Abstract Plant cells can suffer significant damage during cryopreservation, primarily due to extensive ice punctures and oxidative stress from reactive oxygen species (ROS). The extent of damage is closely linked to the moisture content (MC) of seeds, a crucial factor influencing their viability aft...

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Bibliographic Details
Main Authors: Beata P. Plitta-Michalak, Alice A. Ramos, Magdalena Trusiak, Ewelina Ratajczak, Marcin Michalak
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
Subjects:
Comet assay
Cryopreservation
DNA damage
5-hydroxymethylcytosine
5-methylcytosine
8-oxo-7,8-dihydroguanine
Online Access:https://doi.org/10.1038/s41598-025-08476-3
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