Evaluation of DAZ1 Gene Expression in Spermatogenic Failure among Infertile Non-Obstructive Azoospermic Iraqi Males
Background and Objective: Non-obstructive azoospermia (NOA) is one of the causes of male infertility. Male infertility can be induced by changes in the expression pattern of a significant number of genes in spermatogenic cells. So, analysis of gene expression profiles of testicular tissues is essent...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Babol University of Medical Sciences
2024-03-01
|
| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul |
| Subjects: | |
| Online Access: | http://jbums.org/article-1-11375-en.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846129066280747008 |
|---|---|
| author | I Abd Ali Al-Humairi HA Karbal AA Abid Albdairi |
| author_facet | I Abd Ali Al-Humairi HA Karbal AA Abid Albdairi |
| author_sort | I Abd Ali Al-Humairi |
| collection | DOAJ |
| description | Background and Objective: Non-obstructive azoospermia (NOA) is one of the causes of male infertility. Male infertility can be induced by changes in the expression pattern of a significant number of genes in spermatogenic cells. So, analysis of gene expression profiles of testicular tissues is essential in these patients. Since DAZ1 is an essential gene in spermatogenesis, this study aimed to evaluate the role of DAZ1 gene expression in NOA Iraqi males.
Methods: This cross-sectional study enrolled a total of 50 infertile men with NOA attending Teba IVF and genetic Centre from December 2018 to December 2019. All patients had bilateral testicular sperm aspirate (TESA) under local anesthesia and biopsy. The quantitative detection and gene expression analysis of DAZ1 were carried out using the qPCR technique. The correlations between DAZ1 expression levels, testicular spermatogenic patterns, and clinical hormonal indicators were evaluated. The patients' histological phenotype and hormonal profile were also evaluated in gene expression data.
Findings: The mean age of the patients was 35.1±6.6 years. DAZ1 gene expression was different in faulty spermatogenesis testes. Hypospermatogenesis patients had the greatest expression level of DAZ1. DAZ1 transcript expression was significantly lower in the six spermatogenic samples without germ cells or Sertoli cells (p<0.05). Men with the worst histology (maturation arrest and no germ cell and Sertoli cell) had higher mean blood FSH levels (36.6±3.9) (p<0.05). DAZ1 expression in testicular samples demonstrated significant positive correlation with the spermatogenic score (p<0.0001).
Conclusion: The differential expression of spermatogenesis-related gene (DAZ1) across the various spermatogenic patterns suggests that this gene may be a molecular marker for NOA spermatogenesis assessment. |
| format | Article |
| id | doaj-art-ecfc48d750bc4dc9aaad0371439f653f |
| institution | Kabale University |
| issn | 1561-4107 2251-7170 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Babol University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul |
| spelling | doaj-art-ecfc48d750bc4dc9aaad0371439f653f2024-12-10T09:44:47ZengBabol University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul1561-41072251-71702024-03-012600Evaluation of DAZ1 Gene Expression in Spermatogenic Failure among Infertile Non-Obstructive Azoospermic Iraqi MalesI Abd Ali Al-Humairi0HA Karbal1AA Abid Albdairi2 1.Hammurabi College of Medicine, University of Babylon, Babylon, Iraq. 1.Hammurabi College of Medicine, University of Babylon, Babylon, Iraq. 2.Teba IVF & Genetic Center, Babylon, Iraq. Background and Objective: Non-obstructive azoospermia (NOA) is one of the causes of male infertility. Male infertility can be induced by changes in the expression pattern of a significant number of genes in spermatogenic cells. So, analysis of gene expression profiles of testicular tissues is essential in these patients. Since DAZ1 is an essential gene in spermatogenesis, this study aimed to evaluate the role of DAZ1 gene expression in NOA Iraqi males. Methods: This cross-sectional study enrolled a total of 50 infertile men with NOA attending Teba IVF and genetic Centre from December 2018 to December 2019. All patients had bilateral testicular sperm aspirate (TESA) under local anesthesia and biopsy. The quantitative detection and gene expression analysis of DAZ1 were carried out using the qPCR technique. The correlations between DAZ1 expression levels, testicular spermatogenic patterns, and clinical hormonal indicators were evaluated. The patients' histological phenotype and hormonal profile were also evaluated in gene expression data. Findings: The mean age of the patients was 35.1±6.6 years. DAZ1 gene expression was different in faulty spermatogenesis testes. Hypospermatogenesis patients had the greatest expression level of DAZ1. DAZ1 transcript expression was significantly lower in the six spermatogenic samples without germ cells or Sertoli cells (p<0.05). Men with the worst histology (maturation arrest and no germ cell and Sertoli cell) had higher mean blood FSH levels (36.6±3.9) (p<0.05). DAZ1 expression in testicular samples demonstrated significant positive correlation with the spermatogenic score (p<0.0001). Conclusion: The differential expression of spermatogenesis-related gene (DAZ1) across the various spermatogenic patterns suggests that this gene may be a molecular marker for NOA spermatogenesis assessment.http://jbums.org/article-1-11375-en.pdfdaz1 proteinhistopathologymale infertilityspermatogenesisinfertilitynonobstructive azoospermia. |
| spellingShingle | I Abd Ali Al-Humairi HA Karbal AA Abid Albdairi Evaluation of DAZ1 Gene Expression in Spermatogenic Failure among Infertile Non-Obstructive Azoospermic Iraqi Males Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul daz1 protein histopathology male infertility spermatogenesis infertility nonobstructive azoospermia. |
| title | Evaluation of DAZ1 Gene Expression in Spermatogenic Failure among Infertile Non-Obstructive Azoospermic Iraqi Males |
| title_full | Evaluation of DAZ1 Gene Expression in Spermatogenic Failure among Infertile Non-Obstructive Azoospermic Iraqi Males |
| title_fullStr | Evaluation of DAZ1 Gene Expression in Spermatogenic Failure among Infertile Non-Obstructive Azoospermic Iraqi Males |
| title_full_unstemmed | Evaluation of DAZ1 Gene Expression in Spermatogenic Failure among Infertile Non-Obstructive Azoospermic Iraqi Males |
| title_short | Evaluation of DAZ1 Gene Expression in Spermatogenic Failure among Infertile Non-Obstructive Azoospermic Iraqi Males |
| title_sort | evaluation of daz1 gene expression in spermatogenic failure among infertile non obstructive azoospermic iraqi males |
| topic | daz1 protein histopathology male infertility spermatogenesis infertility nonobstructive azoospermia. |
| url | http://jbums.org/article-1-11375-en.pdf |
| work_keys_str_mv | AT iabdalialhumairi evaluationofdaz1geneexpressioninspermatogenicfailureamonginfertilenonobstructiveazoospermiciraqimales AT hakarbal evaluationofdaz1geneexpressioninspermatogenicfailureamonginfertilenonobstructiveazoospermiciraqimales AT aaabidalbdairi evaluationofdaz1geneexpressioninspermatogenicfailureamonginfertilenonobstructiveazoospermiciraqimales |