Optimization of the Ultrasonic-assisted Aqueous Two-phase Extraction of Lycium ruthenicum Polysaccharides and Its Monosaccharide Composition and Pancreatic Lipase Inhibitory Activity
Objective: To optimize the preparation process of polysaccharides from Lycium ruthenicum, analyze the monosaccharide composition and evaluate its pancreatic lipase inhibitory activity in vitro. Methods: Ultrasound-assisted aqueous two-phase extraction method was employed. Taking the overall evaluati...
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The editorial department of Science and Technology of Food Industry
2024-11-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010085 |
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| author | Wan TAN Yangfei WEI Fenglin LI Ning LU Yongpeng GONG Quanchun LI Yi ZHANG |
| author_facet | Wan TAN Yangfei WEI Fenglin LI Ning LU Yongpeng GONG Quanchun LI Yi ZHANG |
| author_sort | Wan TAN |
| collection | DOAJ |
| description | Objective: To optimize the preparation process of polysaccharides from Lycium ruthenicum, analyze the monosaccharide composition and evaluate its pancreatic lipase inhibitory activity in vitro. Methods: Ultrasound-assisted aqueous two-phase extraction method was employed. Taking the overall evaluation value of polysaccharide recovery and extraction rate as the evaluation index, on the basis of single factor experiment, response surface analysis was conducted to optimize the extraction process. The composition of monosaccharides was studied by high performance liquid chromatography with pre-column derivatization. The pancreatic lipase inhibitory activity of Lycium ruthenicum polysaccharides was evaluated by p-nitrophenyl palmitate method. Results: The optimum extraction conditions: Ethanol-ammonium sulfate system, ethanol concentration 30.89%, ammonium sulfate concentration 17.00%, extraction temperature 49.05 ℃, extraction time 81.85 min, liquid to material ratio 29.80 g/g. At this time, the predicted total evaluation value was 0.9242, and the extraction and recovery rates were 104.76 mg/g and 96.96%, respectively. Lycium ruthenicum polysaccharides were composed of monosaccharides such as mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose, with the relative molar ratios of 4.92:0.29:4.80:0.40:9.69:44.79:17.04:18.06, respectively. The IC50 value of Lycium ruthenicum polysaccharides inhibiting pancreatic lipase was 1.51 mg/mL. Conclusion: The preparation process of Lycium ruthenicum polysaccharides optimized by response surface methodology was stable. The detection method of monosaccharide composition was accurate and reliable. Lycium ruthenicum polysaccharides had good pancreatic lipase inhibitory activity in vitro. The results would provide a basis for the further development and utilization of Lycium ruthenicum polysaccharides. |
| format | Article |
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| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2024-11-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-ec13b292f03a40cbacdd946192e4abf12024-11-22T07:28:26ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-11-01452215816810.13386/j.issn1002-0306.20240100852024010085-22Optimization of the Ultrasonic-assisted Aqueous Two-phase Extraction of Lycium ruthenicum Polysaccharides and Its Monosaccharide Composition and Pancreatic Lipase Inhibitory ActivityWan TAN0Yangfei WEI1Fenglin LI2Ning LU3Yongpeng GONG4Quanchun LI5Yi ZHANG6Key Laboratory of Hexi Corridor Resources Utilization of Gansu, Hexi University, Zhangye 734000, ChinaKey Laboratory of Hexi Corridor Resources Utilization of Gansu, Hexi University, Zhangye 734000, ChinaChinese Medicine Hospital of Minle County, Zhangye 734000, ChinaKey Laboratory of Hexi Corridor Resources Utilization of Gansu, Hexi University, Zhangye 734000, ChinaKey Laboratory of Hexi Corridor Resources Utilization of Gansu, Hexi University, Zhangye 734000, ChinaKey Laboratory of Hexi Corridor Resources Utilization of Gansu, Hexi University, Zhangye 734000, ChinaKey Laboratory of Hexi Corridor Resources Utilization of Gansu, Hexi University, Zhangye 734000, ChinaObjective: To optimize the preparation process of polysaccharides from Lycium ruthenicum, analyze the monosaccharide composition and evaluate its pancreatic lipase inhibitory activity in vitro. Methods: Ultrasound-assisted aqueous two-phase extraction method was employed. Taking the overall evaluation value of polysaccharide recovery and extraction rate as the evaluation index, on the basis of single factor experiment, response surface analysis was conducted to optimize the extraction process. The composition of monosaccharides was studied by high performance liquid chromatography with pre-column derivatization. The pancreatic lipase inhibitory activity of Lycium ruthenicum polysaccharides was evaluated by p-nitrophenyl palmitate method. Results: The optimum extraction conditions: Ethanol-ammonium sulfate system, ethanol concentration 30.89%, ammonium sulfate concentration 17.00%, extraction temperature 49.05 ℃, extraction time 81.85 min, liquid to material ratio 29.80 g/g. At this time, the predicted total evaluation value was 0.9242, and the extraction and recovery rates were 104.76 mg/g and 96.96%, respectively. Lycium ruthenicum polysaccharides were composed of monosaccharides such as mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose, with the relative molar ratios of 4.92:0.29:4.80:0.40:9.69:44.79:17.04:18.06, respectively. The IC50 value of Lycium ruthenicum polysaccharides inhibiting pancreatic lipase was 1.51 mg/mL. Conclusion: The preparation process of Lycium ruthenicum polysaccharides optimized by response surface methodology was stable. The detection method of monosaccharide composition was accurate and reliable. Lycium ruthenicum polysaccharides had good pancreatic lipase inhibitory activity in vitro. The results would provide a basis for the further development and utilization of Lycium ruthenicum polysaccharides.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010085lycium ruthenicum murr.polysaccharidesaqueous two-phase extractionresponse surface experimentsmonosaccharide compositionpancreatic lipase |
| spellingShingle | Wan TAN Yangfei WEI Fenglin LI Ning LU Yongpeng GONG Quanchun LI Yi ZHANG Optimization of the Ultrasonic-assisted Aqueous Two-phase Extraction of Lycium ruthenicum Polysaccharides and Its Monosaccharide Composition and Pancreatic Lipase Inhibitory Activity Shipin gongye ke-ji lycium ruthenicum murr. polysaccharides aqueous two-phase extraction response surface experiments monosaccharide composition pancreatic lipase |
| title | Optimization of the Ultrasonic-assisted Aqueous Two-phase Extraction of Lycium ruthenicum Polysaccharides and Its Monosaccharide Composition and Pancreatic Lipase Inhibitory Activity |
| title_full | Optimization of the Ultrasonic-assisted Aqueous Two-phase Extraction of Lycium ruthenicum Polysaccharides and Its Monosaccharide Composition and Pancreatic Lipase Inhibitory Activity |
| title_fullStr | Optimization of the Ultrasonic-assisted Aqueous Two-phase Extraction of Lycium ruthenicum Polysaccharides and Its Monosaccharide Composition and Pancreatic Lipase Inhibitory Activity |
| title_full_unstemmed | Optimization of the Ultrasonic-assisted Aqueous Two-phase Extraction of Lycium ruthenicum Polysaccharides and Its Monosaccharide Composition and Pancreatic Lipase Inhibitory Activity |
| title_short | Optimization of the Ultrasonic-assisted Aqueous Two-phase Extraction of Lycium ruthenicum Polysaccharides and Its Monosaccharide Composition and Pancreatic Lipase Inhibitory Activity |
| title_sort | optimization of the ultrasonic assisted aqueous two phase extraction of lycium ruthenicum polysaccharides and its monosaccharide composition and pancreatic lipase inhibitory activity |
| topic | lycium ruthenicum murr. polysaccharides aqueous two-phase extraction response surface experiments monosaccharide composition pancreatic lipase |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010085 |
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