Molecular detection and phylogenetic analysis of Kenyan human bocavirus isolates
Introduction: The commonly expected causative agents associated with flu-like symptoms in Kenya are the classical viral pathogens identifiable as influenza virus, adenovirus, parainfluenza virus, enteroviruses, respiratory syncytial virus (RSV) and rhinovirus. However, newer agents have been identif...
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The Journal of Infection in Developing Countries
2014-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/3050 |
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| author | Dennis Misigo Dufton Mwaengo David Mburu |
| author_facet | Dennis Misigo Dufton Mwaengo David Mburu |
| author_sort | Dennis Misigo |
| collection | DOAJ |
| description | Introduction: The commonly expected causative agents associated with flu-like symptoms in Kenya are the classical viral pathogens identifiable as influenza virus, adenovirus, parainfluenza virus, enteroviruses, respiratory syncytial virus (RSV) and rhinovirus. However, newer agents have been identified globally that present with illnesses clinically indistinguishable from those caused by the classical pathogens; one of them is human bocavirus.
Methodology: A total of 384 specimens were analyzed, primarily to determine if the emerging human bocavirus (HBoV) infections exist in Kenya as coinfections with other respiratory viruses and to describe the genotype of the virus in circulation. In brief, viral nucleic acids were extracted from culture supernatants, amplified by PCR, and sequenced.
Results: HBoV DNA was amplified from 1.8% of screened specimens. Coinfection with parainfluenza virus, adenovirus, and enterovirus was 2.5%, 2%, and 1.4%, respectively. Multiple coinfections consisting of HBoV plus two other viruses were found in 3% of specimens. Isolation occurred in the months of January, March, April, August, and November. Retrospective review of clinical parameters indicated that all the individuals complained of non-specific symptoms, mainly fever, coughs, nasal stuffiness, runny noses, and vomiting. Phylogenetically, the GenBank deposited sequences of this study’s isolates cluster closely to the reference strain NC_07455 (HBoV1).
Conclusion: Coinfections with human bocavirus (HBoV1) occur in Kenya, and high incidence might primarily be during the early stages of children’s lives.
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| format | Article |
| id | doaj-art-eb8d1fbf6d3b464e94d5ff3db3cc72aa |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2014-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-eb8d1fbf6d3b464e94d5ff3db3cc72aa2025-08-20T03:52:39ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802014-02-0180210.3855/jidc.3050Molecular detection and phylogenetic analysis of Kenyan human bocavirus isolatesDennis Misigo0Dufton Mwaengo1David Mburu2Kenya Methodist University, Meru, KenyaUniversity of Nairobi, Nairobi, KenyaKenyatta University, Nairobi, KenyaIntroduction: The commonly expected causative agents associated with flu-like symptoms in Kenya are the classical viral pathogens identifiable as influenza virus, adenovirus, parainfluenza virus, enteroviruses, respiratory syncytial virus (RSV) and rhinovirus. However, newer agents have been identified globally that present with illnesses clinically indistinguishable from those caused by the classical pathogens; one of them is human bocavirus. Methodology: A total of 384 specimens were analyzed, primarily to determine if the emerging human bocavirus (HBoV) infections exist in Kenya as coinfections with other respiratory viruses and to describe the genotype of the virus in circulation. In brief, viral nucleic acids were extracted from culture supernatants, amplified by PCR, and sequenced. Results: HBoV DNA was amplified from 1.8% of screened specimens. Coinfection with parainfluenza virus, adenovirus, and enterovirus was 2.5%, 2%, and 1.4%, respectively. Multiple coinfections consisting of HBoV plus two other viruses were found in 3% of specimens. Isolation occurred in the months of January, March, April, August, and November. Retrospective review of clinical parameters indicated that all the individuals complained of non-specific symptoms, mainly fever, coughs, nasal stuffiness, runny noses, and vomiting. Phylogenetically, the GenBank deposited sequences of this study’s isolates cluster closely to the reference strain NC_07455 (HBoV1). Conclusion: Coinfections with human bocavirus (HBoV1) occur in Kenya, and high incidence might primarily be during the early stages of children’s lives. https://jidc.org/index.php/journal/article/view/3050human bocavirusKenya isolatesrespiratory viral infections |
| spellingShingle | Dennis Misigo Dufton Mwaengo David Mburu Molecular detection and phylogenetic analysis of Kenyan human bocavirus isolates Journal of Infection in Developing Countries human bocavirus Kenya isolates respiratory viral infections |
| title | Molecular detection and phylogenetic analysis of Kenyan human bocavirus isolates |
| title_full | Molecular detection and phylogenetic analysis of Kenyan human bocavirus isolates |
| title_fullStr | Molecular detection and phylogenetic analysis of Kenyan human bocavirus isolates |
| title_full_unstemmed | Molecular detection and phylogenetic analysis of Kenyan human bocavirus isolates |
| title_short | Molecular detection and phylogenetic analysis of Kenyan human bocavirus isolates |
| title_sort | molecular detection and phylogenetic analysis of kenyan human bocavirus isolates |
| topic | human bocavirus Kenya isolates respiratory viral infections |
| url | https://jidc.org/index.php/journal/article/view/3050 |
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