EMCV VP2 degrades IFI16 through Caspase-dependent apoptosis to evade IFI16-STING pathway
Abstract Interferon (IFN)-γ inducible protein 16 (IFI16), a key DNA sensor, triggers downstream STING-dependent type I interferon (IFN-I) production and antiviral immunity. However, how the IFI16-STING signaling pathway is regulated by EMCV infection is still not well elucidated. In this study, we i...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-11-01
|
| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-024-02568-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846158848935591936 |
|---|---|
| author | Ruofei Feng Dianyu Li Zhenfang Yan Xiangrong Li Jingying Xie |
| author_facet | Ruofei Feng Dianyu Li Zhenfang Yan Xiangrong Li Jingying Xie |
| author_sort | Ruofei Feng |
| collection | DOAJ |
| description | Abstract Interferon (IFN)-γ inducible protein 16 (IFI16), a key DNA sensor, triggers downstream STING-dependent type I interferon (IFN-I) production and antiviral immunity. However, how the IFI16-STING signaling pathway is regulated by EMCV infection is still not well elucidated. In this study, we investigated the interaction between IFI16 and EMCV. Results indicated EMCV infection suppressed IFI16 expression in A549 cells. This study reveals that IFI16 plays an active role in combating EMCV. Screening viral proteins in conjunction with IFI16, we found that the EMCV VP2 protein hinders the antiviral response mediated by IFI16 by causing degradation of the IFI16 protein via the caspase-dependent apoptosis pathway. Our study communicates the antiviral role of the IFI16-STING pathway during EMCV infection. Importantly, this study unveils the novel mechanism by which VP2 counteracts the innate immune signaling activated by foreign DNA. |
| format | Article |
| id | doaj-art-ea256fe9d7064febb7e8adc4c1a9db1c |
| institution | Kabale University |
| issn | 1743-422X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj-art-ea256fe9d7064febb7e8adc4c1a9db1c2024-11-24T12:10:37ZengBMCVirology Journal1743-422X2024-11-0121111710.1186/s12985-024-02568-8EMCV VP2 degrades IFI16 through Caspase-dependent apoptosis to evade IFI16-STING pathwayRuofei Feng0Dianyu Li1Zhenfang Yan2Xiangrong Li3Jingying Xie4Key Laboratory of Biotechnology and Bioengineering of State Ethnic Biomedical Research Center, Northwest Minzu UniversityKey Laboratory of Biotechnology and Bioengineering of State Ethnic Biomedical Research Center, Northwest Minzu UniversityKey Laboratory of Biotechnology and Bioengineering of State Ethnic Biomedical Research Center, Northwest Minzu UniversityKey Laboratory of Biotechnology and Bioengineering of State Ethnic Biomedical Research Center, Northwest Minzu UniversityKey Laboratory of Biotechnology and Bioengineering of State Ethnic Biomedical Research Center, Northwest Minzu UniversityAbstract Interferon (IFN)-γ inducible protein 16 (IFI16), a key DNA sensor, triggers downstream STING-dependent type I interferon (IFN-I) production and antiviral immunity. However, how the IFI16-STING signaling pathway is regulated by EMCV infection is still not well elucidated. In this study, we investigated the interaction between IFI16 and EMCV. Results indicated EMCV infection suppressed IFI16 expression in A549 cells. This study reveals that IFI16 plays an active role in combating EMCV. Screening viral proteins in conjunction with IFI16, we found that the EMCV VP2 protein hinders the antiviral response mediated by IFI16 by causing degradation of the IFI16 protein via the caspase-dependent apoptosis pathway. Our study communicates the antiviral role of the IFI16-STING pathway during EMCV infection. Importantly, this study unveils the novel mechanism by which VP2 counteracts the innate immune signaling activated by foreign DNA.https://doi.org/10.1186/s12985-024-02568-8EMCVIFI16Viral protein VP2Caspase-dependent apoptosis |
| spellingShingle | Ruofei Feng Dianyu Li Zhenfang Yan Xiangrong Li Jingying Xie EMCV VP2 degrades IFI16 through Caspase-dependent apoptosis to evade IFI16-STING pathway Virology Journal EMCV IFI16 Viral protein VP2 Caspase-dependent apoptosis |
| title | EMCV VP2 degrades IFI16 through Caspase-dependent apoptosis to evade IFI16-STING pathway |
| title_full | EMCV VP2 degrades IFI16 through Caspase-dependent apoptosis to evade IFI16-STING pathway |
| title_fullStr | EMCV VP2 degrades IFI16 through Caspase-dependent apoptosis to evade IFI16-STING pathway |
| title_full_unstemmed | EMCV VP2 degrades IFI16 through Caspase-dependent apoptosis to evade IFI16-STING pathway |
| title_short | EMCV VP2 degrades IFI16 through Caspase-dependent apoptosis to evade IFI16-STING pathway |
| title_sort | emcv vp2 degrades ifi16 through caspase dependent apoptosis to evade ifi16 sting pathway |
| topic | EMCV IFI16 Viral protein VP2 Caspase-dependent apoptosis |
| url | https://doi.org/10.1186/s12985-024-02568-8 |
| work_keys_str_mv | AT ruofeifeng emcvvp2degradesifi16throughcaspasedependentapoptosistoevadeifi16stingpathway AT dianyuli emcvvp2degradesifi16throughcaspasedependentapoptosistoevadeifi16stingpathway AT zhenfangyan emcvvp2degradesifi16throughcaspasedependentapoptosistoevadeifi16stingpathway AT xiangrongli emcvvp2degradesifi16throughcaspasedependentapoptosistoevadeifi16stingpathway AT jingyingxie emcvvp2degradesifi16throughcaspasedependentapoptosistoevadeifi16stingpathway |