Fat grafting: Lipofragmentation X Liposuction
Introduction: Aiming to obtain autogenous and injectable lipografts from resected tissues in dermolipectomies, this study proposes a new method for harvesting and processing adipose tissue through a specific fragmenting device. The main objective was to establish a comparative analysis of the qualit...
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| Format: | Article |
| Language: | English |
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Thieme Revinter Publicações Ltda.
2019-09-01
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| Series: | Revista Brasileira de Cirurgia Plástica |
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| Online Access: | http://www.rbcp.org.br/export-pdf/2653/en_v34n3a11.pdf |
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| author | Flavio Henrique Mendes Fausto Viterbo Elenice Deffune Maria Aparecida Custódio Domingues Marjorie Assis Golim José Marcos Gabas Renan Roldi Rossoni; Helga Caputo Nunes |
| author_facet | Flavio Henrique Mendes Fausto Viterbo Elenice Deffune Maria Aparecida Custódio Domingues Marjorie Assis Golim José Marcos Gabas Renan Roldi Rossoni; Helga Caputo Nunes |
| author_sort | Flavio Henrique Mendes |
| collection | DOAJ |
| description | Introduction: Aiming to obtain autogenous and injectable lipografts from resected tissues in dermolipectomies, this study proposes a new method for harvesting and processing adipose tissue through a specific fragmenting device. The main objective was to establish a comparative analysis of the quality and viability characteristics of the new lipofragmentation technique and those of the well-known liposuction technique, widely accepted as a viable source of fat grafting. In vivo and in vitro assays were designed to evaluate the biological behavior of the samples to guide new and possible human studies with clinical applications.
Methods: A post-bariatric patient who underwent abdominal dermolipectomy had her surgical specimen resected, which was divided into four parts that underwent liposuction and lipofragmentation, with and without prior infiltration. All samples were centrifuged and distributed for assays with assessments involving histological analysis, immunohistochemistry, flow cytometry, cell culture, and xenograft injection on the back of 10 Wistar rats, which was evaluated after six weeks for mass, volume, and histological features.
Results: The structural characteristics and biological behaviors of fragmented, dry, and infiltrated fat samples were similar to those of liposuction samples.
Conclusions: Fat fragmentation transformed the subcutaneous cellular tissue of dermolipectomies into a new, viable injectable lipograft variant, with biological characteristics similar to those of traditional liposuction. Although still preliminary, our results support further investigations to optimize the technique and improve fat grafting and its possible applications in regenerative medicine. |
| format | Article |
| id | doaj-art-e9c5cacf6d4f4c4c977a6af9c9f9db31 |
| institution | Kabale University |
| issn | 1983-5175 2177-1235 |
| language | English |
| publishDate | 2019-09-01 |
| publisher | Thieme Revinter Publicações Ltda. |
| record_format | Article |
| series | Revista Brasileira de Cirurgia Plástica |
| spelling | doaj-art-e9c5cacf6d4f4c4c977a6af9c9f9db312025-08-20T03:39:18ZengThieme Revinter Publicações Ltda.Revista Brasileira de Cirurgia Plástica1983-51752177-12352019-09-01340336837710.5935/2177-1235.2019RBCP0210Fat grafting: Lipofragmentation X LiposuctionFlavio Henrique Mendes0Fausto Viterbo1Elenice Deffune2Maria Aparecida Custódio Domingues3Marjorie Assis Golim4José Marcos Gabas5Renan Roldi Rossoni;6Helga Caputo Nunes7Faculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, BrazilFaculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, BrazilFaculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, BrazilFaculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, BrazilFaculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, BrazilFaculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, BrazilFaculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, BrazilFaculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, BrazilIntroduction: Aiming to obtain autogenous and injectable lipografts from resected tissues in dermolipectomies, this study proposes a new method for harvesting and processing adipose tissue through a specific fragmenting device. The main objective was to establish a comparative analysis of the quality and viability characteristics of the new lipofragmentation technique and those of the well-known liposuction technique, widely accepted as a viable source of fat grafting. In vivo and in vitro assays were designed to evaluate the biological behavior of the samples to guide new and possible human studies with clinical applications. Methods: A post-bariatric patient who underwent abdominal dermolipectomy had her surgical specimen resected, which was divided into four parts that underwent liposuction and lipofragmentation, with and without prior infiltration. All samples were centrifuged and distributed for assays with assessments involving histological analysis, immunohistochemistry, flow cytometry, cell culture, and xenograft injection on the back of 10 Wistar rats, which was evaluated after six weeks for mass, volume, and histological features. Results: The structural characteristics and biological behaviors of fragmented, dry, and infiltrated fat samples were similar to those of liposuction samples. Conclusions: Fat fragmentation transformed the subcutaneous cellular tissue of dermolipectomies into a new, viable injectable lipograft variant, with biological characteristics similar to those of traditional liposuction. Although still preliminary, our results support further investigations to optimize the technique and improve fat grafting and its possible applications in regenerative medicine.http://www.rbcp.org.br/export-pdf/2653/en_v34n3a11.pdfadipose tissuetransplantsplastic surgerybioprosthesisgraft survival |
| spellingShingle | Flavio Henrique Mendes Fausto Viterbo Elenice Deffune Maria Aparecida Custódio Domingues Marjorie Assis Golim José Marcos Gabas Renan Roldi Rossoni; Helga Caputo Nunes Fat grafting: Lipofragmentation X Liposuction Revista Brasileira de Cirurgia Plástica adipose tissue transplants plastic surgery bioprosthesis graft survival |
| title | Fat grafting: Lipofragmentation X Liposuction |
| title_full | Fat grafting: Lipofragmentation X Liposuction |
| title_fullStr | Fat grafting: Lipofragmentation X Liposuction |
| title_full_unstemmed | Fat grafting: Lipofragmentation X Liposuction |
| title_short | Fat grafting: Lipofragmentation X Liposuction |
| title_sort | fat grafting lipofragmentation x liposuction |
| topic | adipose tissue transplants plastic surgery bioprosthesis graft survival |
| url | http://www.rbcp.org.br/export-pdf/2653/en_v34n3a11.pdf |
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