Comprehensive analysis of poly(A) tails in mouse testes and ovaries using Nanopore Direct RNA Sequencing
Abstract Gametogenesis is a process in which dysfunctions lead to infertility, a growing health and social problem worldwide. In both spermatogenesis and oogenesis, post-transcriptional gene expression regulation is crucial. Essentially, all mRNAs possess non-templated poly(A) tails, whose compositi...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-024-04226-8 |
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| author | Agnieszka Czarnocka-Cieciura Michał Brouze Natalia Gumińska Seweryn Mroczek Olga Gewartowska Paweł S. Krawczyk Andrzej Dziembowski |
| author_facet | Agnieszka Czarnocka-Cieciura Michał Brouze Natalia Gumińska Seweryn Mroczek Olga Gewartowska Paweł S. Krawczyk Andrzej Dziembowski |
| author_sort | Agnieszka Czarnocka-Cieciura |
| collection | DOAJ |
| description | Abstract Gametogenesis is a process in which dysfunctions lead to infertility, a growing health and social problem worldwide. In both spermatogenesis and oogenesis, post-transcriptional gene expression regulation is crucial. Essentially, all mRNAs possess non-templated poly(A) tails, whose composition and dynamics (elongation, shortening, and modifications) determine the fate of mRNA. Moreover, gametogenesis, especially oogenesis, represents a unique instance of the complexity of poly(A) tails metabolism, with oocyte-specific waves of cytoplasmic polyadenylation. In this context, we provide a comprehensive transcriptomic dataset focusing on mRNA poly(A) tail composition and dynamics in murine testes and ovaries. It consists of RNA samples isolated from wild-type and transgenic mice lacking TENT5 polymerases, which can extend poly(A) tails in the cytoplasm. TENT5 deficiencies have serious consequences. For instance, the defect of TENT5D causes infertility in humans. The data described here are generated mainly using the Oxford Nanopore Direct RNA Sequencing (DRS) method, which provides ground-truth information about mRNA molecules, including poly(A) tail length and nucleotide content. For instance, we show the prevalence of uridilated tails in testicular mRNAs. |
| format | Article |
| id | doaj-art-e7eeecf6a1684003b77a1962f78b5f3c |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-e7eeecf6a1684003b77a1962f78b5f3c2025-01-12T12:07:52ZengNature PortfolioScientific Data2052-44632025-01-0112111010.1038/s41597-024-04226-8Comprehensive analysis of poly(A) tails in mouse testes and ovaries using Nanopore Direct RNA SequencingAgnieszka Czarnocka-Cieciura0Michał Brouze1Natalia Gumińska2Seweryn Mroczek3Olga Gewartowska4Paweł S. Krawczyk5Andrzej Dziembowski6Laboratory of RNA Biology, International Institute of Molecular and Cell BiologyLaboratory of RNA Biology, International Institute of Molecular and Cell BiologyLaboratory of RNA Biology, International Institute of Molecular and Cell BiologyLaboratory of RNA Biology, International Institute of Molecular and Cell BiologyGenome Engineering Facility, International Institute of Molecular and Cell BiologyLaboratory of RNA Biology, International Institute of Molecular and Cell BiologyLaboratory of RNA Biology, International Institute of Molecular and Cell BiologyAbstract Gametogenesis is a process in which dysfunctions lead to infertility, a growing health and social problem worldwide. In both spermatogenesis and oogenesis, post-transcriptional gene expression regulation is crucial. Essentially, all mRNAs possess non-templated poly(A) tails, whose composition and dynamics (elongation, shortening, and modifications) determine the fate of mRNA. Moreover, gametogenesis, especially oogenesis, represents a unique instance of the complexity of poly(A) tails metabolism, with oocyte-specific waves of cytoplasmic polyadenylation. In this context, we provide a comprehensive transcriptomic dataset focusing on mRNA poly(A) tail composition and dynamics in murine testes and ovaries. It consists of RNA samples isolated from wild-type and transgenic mice lacking TENT5 polymerases, which can extend poly(A) tails in the cytoplasm. TENT5 deficiencies have serious consequences. For instance, the defect of TENT5D causes infertility in humans. The data described here are generated mainly using the Oxford Nanopore Direct RNA Sequencing (DRS) method, which provides ground-truth information about mRNA molecules, including poly(A) tail length and nucleotide content. For instance, we show the prevalence of uridilated tails in testicular mRNAs.https://doi.org/10.1038/s41597-024-04226-8 |
| spellingShingle | Agnieszka Czarnocka-Cieciura Michał Brouze Natalia Gumińska Seweryn Mroczek Olga Gewartowska Paweł S. Krawczyk Andrzej Dziembowski Comprehensive analysis of poly(A) tails in mouse testes and ovaries using Nanopore Direct RNA Sequencing Scientific Data |
| title | Comprehensive analysis of poly(A) tails in mouse testes and ovaries using Nanopore Direct RNA Sequencing |
| title_full | Comprehensive analysis of poly(A) tails in mouse testes and ovaries using Nanopore Direct RNA Sequencing |
| title_fullStr | Comprehensive analysis of poly(A) tails in mouse testes and ovaries using Nanopore Direct RNA Sequencing |
| title_full_unstemmed | Comprehensive analysis of poly(A) tails in mouse testes and ovaries using Nanopore Direct RNA Sequencing |
| title_short | Comprehensive analysis of poly(A) tails in mouse testes and ovaries using Nanopore Direct RNA Sequencing |
| title_sort | comprehensive analysis of poly a tails in mouse testes and ovaries using nanopore direct rna sequencing |
| url | https://doi.org/10.1038/s41597-024-04226-8 |
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