miR-155与<italic>CEBPB</italic>的靶向验证及繁殖性能相关基因的检测

miR-155与<italic>CEBPB</italic>的靶向验证及繁殖性能相关基因的检测

<italic>CEBPB</italic>具有调节黄体生成促进排卵的作用，对动物繁殖性能极为重要，试验旨在通过构建<italic>CEBPB</italic>的3'端非翻译区（3'UTR）双荧光素酶报告基因载体，验证MicroRNA-155（miR-155）调控<italic>CEBPB</italic>的分子机制。应用在线生物信息学软件预测miR-155与<italic>CEBPB</italic>基因3'UTR的靶向结合区，PCR扩增获取民猪组织基因组的<i...

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Bibliographic Details
Main Authors: 李军义, 刘娣, 马红, 汪亮, 霍秀鹏, 郝丽
Format: Article
Language:zho
Published: Editorial Department of Chinese Journal of Wildlife 2023-05-01
Series:野生动物学报
Subjects:
繁殖性能
<italic>CEBPB</italic>基因
miR-155
Online Access:http://ysdw.nefu.edu.cn/thesisDetails#10.12375/ysdwxb.20230209
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