Large‐scale image‐based profiling of single‐cell phenotypes in arrayed CRISPR‐Cas9 gene perturbation screens

Large‐scale image‐based profiling of single‐cell phenotypes in arrayed CRISPR‐Cas9 gene perturbation screens

Abstract High‐content imaging using automated microscopy and computer vision allows multivariate profiling of single‐cell phenotypes. Here, we present methods for the application of the CISPR‐Cas9 system in large‐scale, image‐based, gene perturbation experiments. We show that CRISPR‐Cas9‐mediated ge...

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Bibliographic Details
Main Authors: Reinoud de Groot, Joel Lüthi, Helen Lindsay, René Holtackers, Lucas Pelkmans
Format: Article
Language:English
Published: Springer Nature 2018-01-01
Series:Molecular Systems Biology
Subjects:
arrayed library
CRISPR‐Cas9
functional genomics
nuclear pore complex
single‐cell phenotypic profiling
Online Access:https://doi.org/10.15252/msb.20178064
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https://doi.org/10.15252/msb.20178064

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