Influence of different cavity disinfectants on composite adhesion to dentin of primary teeth: An in vitro study

Restorative treatment is the most common approach to treating dental caries. However, after cavity preparation, some microorganisms may still persist in the substrate, suggesting the use of cavity disinfectants. Nevertheless, their effect on adhesion to composite resins is not yet fully understood,...

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Main Authors: Ana Coelho, Luís Vilhena, Mariana Cordeiro, Inês Amaro, Anabela Paula, Carlos Miguel Marto, Cristina Cardoso Silva, Manuel Marques Ferreira, Eunice Carrilho, Amílcar Ramalho
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Heliyon
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024162949
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author Ana Coelho
Luís Vilhena
Mariana Cordeiro
Inês Amaro
Anabela Paula
Carlos Miguel Marto
Cristina Cardoso Silva
Manuel Marques Ferreira
Eunice Carrilho
Amílcar Ramalho
author_facet Ana Coelho
Luís Vilhena
Mariana Cordeiro
Inês Amaro
Anabela Paula
Carlos Miguel Marto
Cristina Cardoso Silva
Manuel Marques Ferreira
Eunice Carrilho
Amílcar Ramalho
author_sort Ana Coelho
collection DOAJ
description Restorative treatment is the most common approach to treating dental caries. However, after cavity preparation, some microorganisms may still persist in the substrate, suggesting the use of cavity disinfectants. Nevertheless, their effect on adhesion to composite resins is not yet fully understood, especially in primary teeth. The study aimed to assess the impact of five different cavity disinfectants on dentin adhesion in primary teeth. A total of 60 primary molars were uniformly flattened at their occlusal thirds and randomly allocated into six groups (n = 10 each): Control; Glutaraldehyde; Chlorhexidine; EDTA; Ethanol; Aloe vera. All disinfectants were actively applied, rinsed, and air-dried. The adhesion procedure was carried out according to the manufacturer's instructions, and the restoration was positioned using a mold. Shear bond strength was evaluated. Data were statistically analyzed (One-way ANOVA with Post-hoc Tukey test), with the level of significance set at 5 %. Glutaraldehyde (14.59 ± 3.89 MPa), Chlorhexidine (11.24 ± 2.25 MPa), and EDTA (11.04 ± 2.95 MPa) did not impair the shear bond strength when compared to the Control group (14.95 ± 2.75 MPa). Ethanol and Aloe vera application significantly lowered SBS. The results suggest that Glutaraldehyde, Chlorhexidine, and EDTA can be used as cavity disinfectants. Nevertheless, further in vitro and clinical research is required.
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spelling doaj-art-e5c73162e3cc4ea6a2c957d700dea2462024-12-13T10:58:37ZengElsevierHeliyon2405-84402024-12-011023e40263Influence of different cavity disinfectants on composite adhesion to dentin of primary teeth: An in vitro studyAna Coelho0Luís Vilhena1Mariana Cordeiro2Inês Amaro3Anabela Paula4Carlos Miguel Marto5Cristina Cardoso Silva6Manuel Marques Ferreira7Eunice Carrilho8Amílcar Ramalho9Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal; Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal; Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal; Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, PortugalDepartment of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, PortugalInstitute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, PortugalInstitute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal; Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal; Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal; Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, PortugalInstitute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal; Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal; Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal; Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, PortugalArea of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal; Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal; Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, Portugal; Institute of Biophysics, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal; Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal; Corresponding author. Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, Portugal.Faculty of Health Sciences, University Fernando Pessoa, 4200-551 Porto, Portugal; FP – I3ID, FP – BHS, Faculty of Health Sciences, University Fernando Pessoa, 4200-551 Porto, PortugalArea of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal; Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal; Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, Portugal; Institute of Endodontics, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, PortugalInstitute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal; Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal; Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal; Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, PortugalDepartment of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, PortugalRestorative treatment is the most common approach to treating dental caries. However, after cavity preparation, some microorganisms may still persist in the substrate, suggesting the use of cavity disinfectants. Nevertheless, their effect on adhesion to composite resins is not yet fully understood, especially in primary teeth. The study aimed to assess the impact of five different cavity disinfectants on dentin adhesion in primary teeth. A total of 60 primary molars were uniformly flattened at their occlusal thirds and randomly allocated into six groups (n = 10 each): Control; Glutaraldehyde; Chlorhexidine; EDTA; Ethanol; Aloe vera. All disinfectants were actively applied, rinsed, and air-dried. The adhesion procedure was carried out according to the manufacturer's instructions, and the restoration was positioned using a mold. Shear bond strength was evaluated. Data were statistically analyzed (One-way ANOVA with Post-hoc Tukey test), with the level of significance set at 5 %. Glutaraldehyde (14.59 ± 3.89 MPa), Chlorhexidine (11.24 ± 2.25 MPa), and EDTA (11.04 ± 2.95 MPa) did not impair the shear bond strength when compared to the Control group (14.95 ± 2.75 MPa). Ethanol and Aloe vera application significantly lowered SBS. The results suggest that Glutaraldehyde, Chlorhexidine, and EDTA can be used as cavity disinfectants. Nevertheless, further in vitro and clinical research is required.http://www.sciencedirect.com/science/article/pii/S2405844024162949AdhesionCavity disinfectantsIn vitro techniquePrimary teethShear bond strength
spellingShingle Ana Coelho
Luís Vilhena
Mariana Cordeiro
Inês Amaro
Anabela Paula
Carlos Miguel Marto
Cristina Cardoso Silva
Manuel Marques Ferreira
Eunice Carrilho
Amílcar Ramalho
Influence of different cavity disinfectants on composite adhesion to dentin of primary teeth: An in vitro study
Heliyon
Adhesion
Cavity disinfectants
In vitro technique
Primary teeth
Shear bond strength
title Influence of different cavity disinfectants on composite adhesion to dentin of primary teeth: An in vitro study
title_full Influence of different cavity disinfectants on composite adhesion to dentin of primary teeth: An in vitro study
title_fullStr Influence of different cavity disinfectants on composite adhesion to dentin of primary teeth: An in vitro study
title_full_unstemmed Influence of different cavity disinfectants on composite adhesion to dentin of primary teeth: An in vitro study
title_short Influence of different cavity disinfectants on composite adhesion to dentin of primary teeth: An in vitro study
title_sort influence of different cavity disinfectants on composite adhesion to dentin of primary teeth an in vitro study
topic Adhesion
Cavity disinfectants
In vitro technique
Primary teeth
Shear bond strength
url http://www.sciencedirect.com/science/article/pii/S2405844024162949
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