High β-sitosterol-D-glucoside content in sweet potato varieties and its anti-breast cancer potential through multiple metastasis-associated signals
β-Sitosterol-D-glucoside (β-SDG) is a phytosterol compound whose antitumor activity has been confirmed by previous studies. However, its suppression on breast cancer remains unclear. To that purpose, we isolated β-SDG from sweet potato and investigated the breast-cancer-inhibiting mechanism using pr...
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| Format: | Article |
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Tsinghua University Press
2024-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250225 |
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| author | Jinyin Zhang Lu Huang Xiang Tang Miao Pan Xiaoli Ye Xuegang Li Hang Ma |
| author_facet | Jinyin Zhang Lu Huang Xiang Tang Miao Pan Xiaoli Ye Xuegang Li Hang Ma |
| author_sort | Jinyin Zhang |
| collection | DOAJ |
| description | β-Sitosterol-D-glucoside (β-SDG) is a phytosterol compound whose antitumor activity has been confirmed by previous studies. However, its suppression on breast cancer remains unclear. To that purpose, we isolated β-SDG from sweet potato and investigated the breast-cancer-inhibiting mechanism using proteomic analysis. The sweet potato species S6 with high β-SDG content were chosen form 36 species and β-SDG was isolated by HPLC. Afterwards, an in situ animal model of breast cancer was established, and β-SDG significantly reduced the tumor volume of MCF-7 xenograft mice. Proteomic analysis of tumor tissues revealed that 127 of these proteins were upregulated and 80 were downregulated. Gene ontology and network analysis showed that regulatory proteins were mainly associated with epithelial-mesenchymal transition (EMT), myogenesis, cholesterol homeostasis, oxidative phosphorylation and reactive oxygen pathways, while Vimentin, NDUF, VDAC1, PPP2CA and SNx9 were the most significant 5 node degree genes. Meanwhile, in vitro and in vivo results showed that the protein expression of PPP2CA and Vimentin, which are markers of EMT, were involved in breast cancer cell metastasis and could be reversed by β-SDG. This work highlights β-SDG as a bioactive compound in sweet potato and the potential therapeutic effect of β-SDG for the treatment of breast cancer by inhibiting metastasis. |
| format | Article |
| id | doaj-art-e594e481dab84abfa454f375162908d7 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-e594e481dab84abfa454f375162908d72025-01-10T06:56:56ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-09-011352779278910.26599/FSHW.2022.9250225High β-sitosterol-D-glucoside content in sweet potato varieties and its anti-breast cancer potential through multiple metastasis-associated signalsJinyin Zhang0Lu Huang1Xiang Tang2Miao Pan3Xiaoli Ye4Xuegang Li5Hang Ma6School of Pharmaceutical Sciences, Southwest University, Chongqing 400716, ChinaSchool of Life Sciences, Southwest University, Chongqing 400715, ChinaSchool of Life Sciences, Southwest University, Chongqing 400715, ChinaSchool of Pharmaceutical Sciences, Southwest University, Chongqing 400716, ChinaSchool of Life Sciences, Southwest University, Chongqing 400715, ChinaSchool of Pharmaceutical Sciences, Southwest University, Chongqing 400716, ChinaSchool of Pharmaceutical Sciences, Southwest University, Chongqing 400716, Chinaβ-Sitosterol-D-glucoside (β-SDG) is a phytosterol compound whose antitumor activity has been confirmed by previous studies. However, its suppression on breast cancer remains unclear. To that purpose, we isolated β-SDG from sweet potato and investigated the breast-cancer-inhibiting mechanism using proteomic analysis. The sweet potato species S6 with high β-SDG content were chosen form 36 species and β-SDG was isolated by HPLC. Afterwards, an in situ animal model of breast cancer was established, and β-SDG significantly reduced the tumor volume of MCF-7 xenograft mice. Proteomic analysis of tumor tissues revealed that 127 of these proteins were upregulated and 80 were downregulated. Gene ontology and network analysis showed that regulatory proteins were mainly associated with epithelial-mesenchymal transition (EMT), myogenesis, cholesterol homeostasis, oxidative phosphorylation and reactive oxygen pathways, while Vimentin, NDUF, VDAC1, PPP2CA and SNx9 were the most significant 5 node degree genes. Meanwhile, in vitro and in vivo results showed that the protein expression of PPP2CA and Vimentin, which are markers of EMT, were involved in breast cancer cell metastasis and could be reversed by β-SDG. This work highlights β-SDG as a bioactive compound in sweet potato and the potential therapeutic effect of β-SDG for the treatment of breast cancer by inhibiting metastasis.https://www.sciopen.com/article/10.26599/FSHW.2022.9250225β-sitosterol-d-glucoside (β-sdg)sweet potatobreast cancerproteomicmetastasis |
| spellingShingle | Jinyin Zhang Lu Huang Xiang Tang Miao Pan Xiaoli Ye Xuegang Li Hang Ma High β-sitosterol-D-glucoside content in sweet potato varieties and its anti-breast cancer potential through multiple metastasis-associated signals Food Science and Human Wellness β-sitosterol-d-glucoside (β-sdg) sweet potato breast cancer proteomic metastasis |
| title | High β-sitosterol-D-glucoside content in sweet potato varieties and its anti-breast cancer potential through multiple metastasis-associated signals |
| title_full | High β-sitosterol-D-glucoside content in sweet potato varieties and its anti-breast cancer potential through multiple metastasis-associated signals |
| title_fullStr | High β-sitosterol-D-glucoside content in sweet potato varieties and its anti-breast cancer potential through multiple metastasis-associated signals |
| title_full_unstemmed | High β-sitosterol-D-glucoside content in sweet potato varieties and its anti-breast cancer potential through multiple metastasis-associated signals |
| title_short | High β-sitosterol-D-glucoside content in sweet potato varieties and its anti-breast cancer potential through multiple metastasis-associated signals |
| title_sort | high β sitosterol d glucoside content in sweet potato varieties and its anti breast cancer potential through multiple metastasis associated signals |
| topic | β-sitosterol-d-glucoside (β-sdg) sweet potato breast cancer proteomic metastasis |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250225 |
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