分枝杆菌在液体培养基中的L型诱导
通过对不同生长时期的卡介苗及耻垢分枝杆菌以不同组合和浓度的D-环丝氨酸(cyc)和氨苄青霉素(AMP)进行L型诱导,综合染色镜检、菌落形态观察及计数,以及对十二烷基磺酸钠(SDS)敏感性测定的结果,发现在对数生长晚期(卡介苗为OD600=0.25~0.30,耻垢菌为OD600=1.0~1.4)开始诱导,cyc和AMP的浓度分别为1.0mg/ml及0.lmg/ml时诱导效果最好,诱导率可达80%左右。...
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Format: | Article |
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Language: | zho |
Published: |
Editorial Office of Journal of Sun Yat-sen University
1991-01-01
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Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
Subjects: | |
Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43592921/ |
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