Efficient DNA-free co-targeting of nuclear genes in Chlamydomonas reinhardtii

Efficient DNA-free co-targeting of nuclear genes in Chlamydomonas reinhardtii

Abstract Chlamydomonas reinhardtii, a model organism for unicellular green microalgae, is widely used in basic and applied research. Nonetheless, proceeding towards synthetic biology requires a full set of manipulation techniques for inserting, removing, or editing genes. Despite recent advancements...

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Bibliographic Details
Main Authors: Claudia Battarra, Max Angstenberger, Roberto Bassi, Luca Dall’Osto
Format: Article
Language:English
Published: BMC 2024-11-01
Series:Biology Direct
Subjects:
Chlamydomonas reinhardtii
Microalgae
Genome editing
CRISPR/Cas9
Nonhomologous end joining
Antibiotic-free
Online Access:https://doi.org/10.1186/s13062-024-00545-3
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https://doi.org/10.1186/s13062-024-00545-3

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