Dileucine ingestion, but not leucine, increases lower body strength and performance following resistance training: A double-blind, randomized, placebo-controlled trial.
<h4>Background</h4>The essential amino acid leucine (LEU) plays a crucial role in promoting resistance-training adaptations. Dileucine (DILEU), a LEU-LEU dipeptide, increases MPS rates, however its impact on resistance training outcomes remains unexplored. This study assessed the effects...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2024-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0312997 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841533127998046208 |
|---|---|
| author | Anthony M Hagele Joesi M Krieger Connor J Gaige Kevin F Holley Kristen N Gross Joshua M Iannotti Leah E Allen Paige J Sutton Logan S Orr Petey W Mumford Martin Purpura Ralf Jager Chad M Kerksick |
| author_facet | Anthony M Hagele Joesi M Krieger Connor J Gaige Kevin F Holley Kristen N Gross Joshua M Iannotti Leah E Allen Paige J Sutton Logan S Orr Petey W Mumford Martin Purpura Ralf Jager Chad M Kerksick |
| author_sort | Anthony M Hagele |
| collection | DOAJ |
| description | <h4>Background</h4>The essential amino acid leucine (LEU) plays a crucial role in promoting resistance-training adaptations. Dileucine (DILEU), a LEU-LEU dipeptide, increases MPS rates, however its impact on resistance training outcomes remains unexplored. This study assessed the effects of DILEU supplementation on resistance training adaptations.<h4>Methods</h4>Using a randomized, double-blind, placebo-controlled approach, 34 resistance-trained males (age: 28.3 ± 5.9 years) consumed 2 grams of either DILEU monohydrate (RAMPS™, Ingenious Ingredients, L.P.), LEU, or placebo (PLA) while following a 4-day per week resistance training program for 10 weeks. Changes in body composition, 1-repetition maximum (1RM) and repetitions to failure (RTF) for leg press (LP) and bench press (BP), anaerobic capacity, countermovement jump (CMJ), and maximal voluntary contraction (MVC) were assessed after 0 and 10 weeks.<h4>Results</h4>Significant main effects for time (p < 0.001) were realized for LP and BP 1RM and RTF. A significant group × time interaction was identified for changes in LP 1RM (p = 0.02) and LP RTF (p = 0.03). Greater increases in LP 1RM were observed in DILEU compared to PLA (p = 0.02; 95% CI: 5.8, 73.2 kg), and greater increases in LP RTF in DILEU compared to LEU (p = 0.04; 95% CI: 0.58, 20.3 reps). No significant differences were found in other measures.<h4>Conclusions</h4>DILEU supplementation at 2 grams daily enhanced lower body strength and muscular endurance in resistance-trained males more effectively than LEU or PLA. These findings suggest DILEU as a potentially effective supplement for improving adaptations to resistance training. NCT06121869 retrospectively registered. |
| format | Article |
| id | doaj-art-e011456897524e0ea205ac3adb658510 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-e011456897524e0ea205ac3adb6585102025-01-17T05:31:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031299710.1371/journal.pone.0312997Dileucine ingestion, but not leucine, increases lower body strength and performance following resistance training: A double-blind, randomized, placebo-controlled trial.Anthony M HageleJoesi M KriegerConnor J GaigeKevin F HolleyKristen N GrossJoshua M IannottiLeah E AllenPaige J SuttonLogan S OrrPetey W MumfordMartin PurpuraRalf JagerChad M Kerksick<h4>Background</h4>The essential amino acid leucine (LEU) plays a crucial role in promoting resistance-training adaptations. Dileucine (DILEU), a LEU-LEU dipeptide, increases MPS rates, however its impact on resistance training outcomes remains unexplored. This study assessed the effects of DILEU supplementation on resistance training adaptations.<h4>Methods</h4>Using a randomized, double-blind, placebo-controlled approach, 34 resistance-trained males (age: 28.3 ± 5.9 years) consumed 2 grams of either DILEU monohydrate (RAMPS™, Ingenious Ingredients, L.P.), LEU, or placebo (PLA) while following a 4-day per week resistance training program for 10 weeks. Changes in body composition, 1-repetition maximum (1RM) and repetitions to failure (RTF) for leg press (LP) and bench press (BP), anaerobic capacity, countermovement jump (CMJ), and maximal voluntary contraction (MVC) were assessed after 0 and 10 weeks.<h4>Results</h4>Significant main effects for time (p < 0.001) were realized for LP and BP 1RM and RTF. A significant group × time interaction was identified for changes in LP 1RM (p = 0.02) and LP RTF (p = 0.03). Greater increases in LP 1RM were observed in DILEU compared to PLA (p = 0.02; 95% CI: 5.8, 73.2 kg), and greater increases in LP RTF in DILEU compared to LEU (p = 0.04; 95% CI: 0.58, 20.3 reps). No significant differences were found in other measures.<h4>Conclusions</h4>DILEU supplementation at 2 grams daily enhanced lower body strength and muscular endurance in resistance-trained males more effectively than LEU or PLA. These findings suggest DILEU as a potentially effective supplement for improving adaptations to resistance training. NCT06121869 retrospectively registered.https://doi.org/10.1371/journal.pone.0312997 |
| spellingShingle | Anthony M Hagele Joesi M Krieger Connor J Gaige Kevin F Holley Kristen N Gross Joshua M Iannotti Leah E Allen Paige J Sutton Logan S Orr Petey W Mumford Martin Purpura Ralf Jager Chad M Kerksick Dileucine ingestion, but not leucine, increases lower body strength and performance following resistance training: A double-blind, randomized, placebo-controlled trial. PLoS ONE |
| title | Dileucine ingestion, but not leucine, increases lower body strength and performance following resistance training: A double-blind, randomized, placebo-controlled trial. |
| title_full | Dileucine ingestion, but not leucine, increases lower body strength and performance following resistance training: A double-blind, randomized, placebo-controlled trial. |
| title_fullStr | Dileucine ingestion, but not leucine, increases lower body strength and performance following resistance training: A double-blind, randomized, placebo-controlled trial. |
| title_full_unstemmed | Dileucine ingestion, but not leucine, increases lower body strength and performance following resistance training: A double-blind, randomized, placebo-controlled trial. |
| title_short | Dileucine ingestion, but not leucine, increases lower body strength and performance following resistance training: A double-blind, randomized, placebo-controlled trial. |
| title_sort | dileucine ingestion but not leucine increases lower body strength and performance following resistance training a double blind randomized placebo controlled trial |
| url | https://doi.org/10.1371/journal.pone.0312997 |
| work_keys_str_mv | AT anthonymhagele dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial AT joesimkrieger dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial AT connorjgaige dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial AT kevinfholley dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial AT kristenngross dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial AT joshuamiannotti dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial AT leaheallen dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial AT paigejsutton dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial AT logansorr dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial AT peteywmumford dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial AT martinpurpura dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial AT ralfjager dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial AT chadmkerksick dileucineingestionbutnotleucineincreaseslowerbodystrengthandperformancefollowingresistancetrainingadoubleblindrandomizedplacebocontrolledtrial |