ReBiA—Robotic Enabled Biological Automation: 3D Epithelial Tissue Production
Abstract The Food and Drug Administration's recent decision to eliminate mandatory animal testing for drug approval marks a significant shift to alternative methods. Similarly, the European Parliament is advocating for a faster transition, reflecting public preference for animal‐free research p...
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| Format: | Article |
| Language: | English |
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Wiley
2024-12-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202406608 |
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| author | Lukas Königer Christoph Malkmus Dalia Mahdy Thomas Däullary Susanna Götz Thomas Schwarz Marius Gensler Niklas Pallmann Danjouma Cheufou Andreas Rosenwald Marc Möllmann Dieter Groneberg Christina Popp Florian Groeber‐Becker Maria Steinke Jan Hansmann |
| author_facet | Lukas Königer Christoph Malkmus Dalia Mahdy Thomas Däullary Susanna Götz Thomas Schwarz Marius Gensler Niklas Pallmann Danjouma Cheufou Andreas Rosenwald Marc Möllmann Dieter Groneberg Christina Popp Florian Groeber‐Becker Maria Steinke Jan Hansmann |
| author_sort | Lukas Königer |
| collection | DOAJ |
| description | Abstract The Food and Drug Administration's recent decision to eliminate mandatory animal testing for drug approval marks a significant shift to alternative methods. Similarly, the European Parliament is advocating for a faster transition, reflecting public preference for animal‐free research practices. In vitro tissue models are increasingly recognized as valuable tools for regulatory assessments before clinical trials, in line with the 3R principles (Replace, Reduce, Refine). Despite their potential, barriers such as the need for standardization, availability, and cost hinder their widespread adoption. To address these challenges, the Robotic Enabled Biological Automation (ReBiA) system is developed. This system uses a dual‐arm robot capable of standardizing laboratory processes within a closed automated environment, translating manual processes into automated ones. This reduces the need for process‐specific developments, making in vitro tissue models more consistent and cost‐effective. ReBiA's performance is demonstrated through producing human reconstructed epidermis, human airway epithelial models, and human intestinal organoids. Analyses confirm that these models match the morphology and protein expression of manually prepared and native tissues, with similar cell viability. These successes highlight ReBiA's potential to lower barriers to broader adoption of in vitro tissue models, supporting a shift toward more ethical and advanced research methods. |
| format | Article |
| id | doaj-art-dfe32480d69041d8bc0b2fb1f53adc4c |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-dfe32480d69041d8bc0b2fb1f53adc4c2024-12-04T12:14:54ZengWileyAdvanced Science2198-38442024-12-011145n/an/a10.1002/advs.202406608ReBiA—Robotic Enabled Biological Automation: 3D Epithelial Tissue ProductionLukas Königer0Christoph Malkmus1Dalia Mahdy2Thomas Däullary3Susanna Götz4Thomas Schwarz5Marius Gensler6Niklas Pallmann7Danjouma Cheufou8Andreas Rosenwald9Marc Möllmann10Dieter Groneberg11Christina Popp12Florian Groeber‐Becker13Maria Steinke14Jan Hansmann15Translational Center Regenerative Therapies Fraunhofer Institute for Silicate Research ISC 97070 Würzburg GermanyTranslational Center Regenerative Therapies Fraunhofer Institute for Silicate Research ISC 97070 Würzburg GermanyChair of Tissue Engineering and Regenerative Medicine University Hospital Würzburg 97070 Würzburg GermanyChair of Tissue Engineering and Regenerative Medicine University Hospital Würzburg 97070 Würzburg GermanyFaculty of Design Würzburg Technical University of Applied Sciences Würzburg‐Schweinfurt 97070 Würzburg GermanyTranslational Center Regenerative Therapies Fraunhofer Institute for Silicate Research ISC 97070 Würzburg GermanyChair of Tissue Engineering and Regenerative Medicine University Hospital Würzburg 97070 Würzburg GermanyChair of Tissue Engineering and Regenerative Medicine University Hospital Würzburg 97070 Würzburg GermanyDepartment of Thoracic Surgery Klinikum Würzburg Mitte 97070 Würzburg GermanyInstitute of Pathology University of Würzburg 97080 Würzburg GermanyTranslational Center Regenerative Therapies Fraunhofer Institute for Silicate Research ISC 97070 Würzburg GermanyTranslational Center Regenerative Therapies Fraunhofer Institute for Silicate Research ISC 97070 Würzburg GermanyTranslational Center Regenerative Therapies Fraunhofer Institute for Silicate Research ISC 97070 Würzburg GermanyTranslational Center Regenerative Therapies Fraunhofer Institute for Silicate Research ISC 97070 Würzburg GermanyTranslational Center Regenerative Therapies Fraunhofer Institute for Silicate Research ISC 97070 Würzburg GermanyTranslational Center Regenerative Therapies Fraunhofer Institute for Silicate Research ISC 97070 Würzburg GermanyAbstract The Food and Drug Administration's recent decision to eliminate mandatory animal testing for drug approval marks a significant shift to alternative methods. Similarly, the European Parliament is advocating for a faster transition, reflecting public preference for animal‐free research practices. In vitro tissue models are increasingly recognized as valuable tools for regulatory assessments before clinical trials, in line with the 3R principles (Replace, Reduce, Refine). Despite their potential, barriers such as the need for standardization, availability, and cost hinder their widespread adoption. To address these challenges, the Robotic Enabled Biological Automation (ReBiA) system is developed. This system uses a dual‐arm robot capable of standardizing laboratory processes within a closed automated environment, translating manual processes into automated ones. This reduces the need for process‐specific developments, making in vitro tissue models more consistent and cost‐effective. ReBiA's performance is demonstrated through producing human reconstructed epidermis, human airway epithelial models, and human intestinal organoids. Analyses confirm that these models match the morphology and protein expression of manually prepared and native tissues, with similar cell viability. These successes highlight ReBiA's potential to lower barriers to broader adoption of in vitro tissue models, supporting a shift toward more ethical and advanced research methods.https://doi.org/10.1002/advs.2024066083RAI toolsalternatives to animal testinglab automationtissue engineering |
| spellingShingle | Lukas Königer Christoph Malkmus Dalia Mahdy Thomas Däullary Susanna Götz Thomas Schwarz Marius Gensler Niklas Pallmann Danjouma Cheufou Andreas Rosenwald Marc Möllmann Dieter Groneberg Christina Popp Florian Groeber‐Becker Maria Steinke Jan Hansmann ReBiA—Robotic Enabled Biological Automation: 3D Epithelial Tissue Production Advanced Science 3R AI tools alternatives to animal testing lab automation tissue engineering |
| title | ReBiA—Robotic Enabled Biological Automation: 3D Epithelial Tissue Production |
| title_full | ReBiA—Robotic Enabled Biological Automation: 3D Epithelial Tissue Production |
| title_fullStr | ReBiA—Robotic Enabled Biological Automation: 3D Epithelial Tissue Production |
| title_full_unstemmed | ReBiA—Robotic Enabled Biological Automation: 3D Epithelial Tissue Production |
| title_short | ReBiA—Robotic Enabled Biological Automation: 3D Epithelial Tissue Production |
| title_sort | rebia robotic enabled biological automation 3d epithelial tissue production |
| topic | 3R AI tools alternatives to animal testing lab automation tissue engineering |
| url | https://doi.org/10.1002/advs.202406608 |
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