Surface-tailored graphene nanosheets targeting PI3K/Akt signaling of breast cancer cells
Abstract Herein, we report graphene nanosheets fabricated using liquid-phase exfoliation of graphite employing cetyltrimethylammonium chloride (CTAC) in an aqueous medium. The salient features of CTAC-assisted graphene nanosheets are as follows: (i) the concentration of graphene dispersed in water i...
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Nature Portfolio
2025-08-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-00983-7 |
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| author | Sabapathi Durgadevi Ponnuchamy Kumar |
| author_facet | Sabapathi Durgadevi Ponnuchamy Kumar |
| author_sort | Sabapathi Durgadevi |
| collection | DOAJ |
| description | Abstract Herein, we report graphene nanosheets fabricated using liquid-phase exfoliation of graphite employing cetyltrimethylammonium chloride (CTAC) in an aqueous medium. The salient features of CTAC-assisted graphene nanosheets are as follows: (i) the concentration of graphene dispersed in water is found to be 26.01 mg/mL at 50 mg with a Critical Micelle Concentration (CMC) Value of 3 mg/mL (9.375 mM); (ii) rapid exfoliation is assisted by CTAC via sonication, which develops hydrophilic micelles on graphitic surface; (iii) the strong repulsive force of CTAC prevents re-aggregation of graphene sheets by means of electrostatic interaction as revealed by zeta potential measurement (+ 33.8 mV); (iv) the graphene nanosheets display excellent anticancer properties against MCF-7 (IC50 – 205.5 ng/mL) and MDA-MB-231 (IC50 – 216.9 ng/mL) breast cancer cells; (v) the CTAC assisted graphene nanosheets induce PI3K/Akt mediated apoptosis in breast cancer cells. Based on the results, CTAC-assisted graphene nanosheets are expected to be a potential candidate for therapeutic applications in the near future. |
| format | Article |
| id | doaj-art-df5d7f9d1c114dfdadff775715ff9db2 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-df5d7f9d1c114dfdadff775715ff9db22025-08-20T03:42:48ZengNature PortfolioScientific Reports2045-23222025-08-0115111410.1038/s41598-025-00983-7Surface-tailored graphene nanosheets targeting PI3K/Akt signaling of breast cancer cellsSabapathi Durgadevi0Ponnuchamy Kumar1Food Chemistry and Molecular Cancer Biology, Department of Animal Health and Management, Alagappa UniversityFood Chemistry and Molecular Cancer Biology, Department of Animal Health and Management, Alagappa UniversityAbstract Herein, we report graphene nanosheets fabricated using liquid-phase exfoliation of graphite employing cetyltrimethylammonium chloride (CTAC) in an aqueous medium. The salient features of CTAC-assisted graphene nanosheets are as follows: (i) the concentration of graphene dispersed in water is found to be 26.01 mg/mL at 50 mg with a Critical Micelle Concentration (CMC) Value of 3 mg/mL (9.375 mM); (ii) rapid exfoliation is assisted by CTAC via sonication, which develops hydrophilic micelles on graphitic surface; (iii) the strong repulsive force of CTAC prevents re-aggregation of graphene sheets by means of electrostatic interaction as revealed by zeta potential measurement (+ 33.8 mV); (iv) the graphene nanosheets display excellent anticancer properties against MCF-7 (IC50 – 205.5 ng/mL) and MDA-MB-231 (IC50 – 216.9 ng/mL) breast cancer cells; (v) the CTAC assisted graphene nanosheets induce PI3K/Akt mediated apoptosis in breast cancer cells. Based on the results, CTAC-assisted graphene nanosheets are expected to be a potential candidate for therapeutic applications in the near future.https://doi.org/10.1038/s41598-025-00983-7Liquid-phase exfoliationCTACGrapheneBreast cancerApoptosisPI3K/Akt signaling |
| spellingShingle | Sabapathi Durgadevi Ponnuchamy Kumar Surface-tailored graphene nanosheets targeting PI3K/Akt signaling of breast cancer cells Scientific Reports Liquid-phase exfoliation CTAC Graphene Breast cancer Apoptosis PI3K/Akt signaling |
| title | Surface-tailored graphene nanosheets targeting PI3K/Akt signaling of breast cancer cells |
| title_full | Surface-tailored graphene nanosheets targeting PI3K/Akt signaling of breast cancer cells |
| title_fullStr | Surface-tailored graphene nanosheets targeting PI3K/Akt signaling of breast cancer cells |
| title_full_unstemmed | Surface-tailored graphene nanosheets targeting PI3K/Akt signaling of breast cancer cells |
| title_short | Surface-tailored graphene nanosheets targeting PI3K/Akt signaling of breast cancer cells |
| title_sort | surface tailored graphene nanosheets targeting pi3k akt signaling of breast cancer cells |
| topic | Liquid-phase exfoliation CTAC Graphene Breast cancer Apoptosis PI3K/Akt signaling |
| url | https://doi.org/10.1038/s41598-025-00983-7 |
| work_keys_str_mv | AT sabapathidurgadevi surfacetailoredgraphenenanosheetstargetingpi3kaktsignalingofbreastcancercells AT ponnuchamykumar surfacetailoredgraphenenanosheetstargetingpi3kaktsignalingofbreastcancercells |