Protocol for conditional mutagenesis in zebrafish germ cells using Tol2 transposon and a CRISPR-Cas9-based plasmid system

Summary: Here, we present a protocol for conditional mutagenesis in zebrafish germ cells using Tol2 transposon and a CRISPR-Cas9-based plasmid system. We describe steps for conditional mutagenesis plasmid construction, zebrafish embryo microinjection, and screening for green fluorescence in the hear...

Full description

Saved in:
Bibliographic Details
Main Authors: Yixuan Hu, Yutao Chen, Yu Zhang, Zhiquan Liu, Jianzhen Li
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724006816
Tags: Add Tag
No Tags, Be the first to tag this record!