The Immunosuppressive Functions of Eosinophils Are Compromised in Patients With Allergic Rhinitis, Particularly Concerning Rab27a Expression
ABSTRACT Background Eosinophils have been acknowledged to be involved in the induction of numerous inflammatory disorders. There is still a lack of knowledge about whether eosinophils play a role in immune regulation. The aim of this study is to uncover the immune regulatory functions of eosinophils...
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Wiley
2024-12-01
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| Series: | Immunity, Inflammation and Disease |
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| Online Access: | https://doi.org/10.1002/iid3.70091 |
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| author | Yun Liao Minyao Li Shuo Song Xuejie Xu Xiaojun Xiao Yu Liu Gui Yang Pingchang Yang |
| author_facet | Yun Liao Minyao Li Shuo Song Xuejie Xu Xiaojun Xiao Yu Liu Gui Yang Pingchang Yang |
| author_sort | Yun Liao |
| collection | DOAJ |
| description | ABSTRACT Background Eosinophils have been acknowledged to be involved in the induction of numerous inflammatory disorders. There is still a lack of knowledge about whether eosinophils play a role in immune regulation. The aim of this study is to uncover the immune regulatory functions of eosinophils. Methods Blood samples were collected from patients with allergic rhinitis (AR) and healthy control subjects. Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples. Eosinophils were purified from PBMCs using flow cytometry cell sorting and analyzed using immunological approaches. Results The results showed that eosinophils from healthy subjects had immune regulatory functions on T cell proliferation and cytokine release. Impairment of eosinophil immune regulatory functions was found in AR patients, which was associated with AR responses. Elevated Rab27a expression in eosinophils was associated with their impaired immune regulatory functions and the increased AR responses. Rab27a controlled the release of mediators from eosinophils. Low concentrations of Eosinophil mediators could trigger immune regulatory responses, while high concentrations could trigger inflammatory responses. Regulating Rab27a restored the immune regulatory functions of eosinophils of AR patients. Conclusions Eosinophils have immune regulatory functions, which are controlled by the expression of Rab27a. Regulation of Rab27a can improve the immune regulatory functions of eosinophils. The data suggest that inhibition of Rab27a can be a drug candidate for the treatment of eosinophil‐related disorders. |
| format | Article |
| id | doaj-art-dc6cad6d56c84428bbc6a02e6b24dc01 |
| institution | Kabale University |
| issn | 2050-4527 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
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| series | Immunity, Inflammation and Disease |
| spelling | doaj-art-dc6cad6d56c84428bbc6a02e6b24dc012024-12-26T14:35:28ZengWileyImmunity, Inflammation and Disease2050-45272024-12-011212n/an/a10.1002/iid3.70091The Immunosuppressive Functions of Eosinophils Are Compromised in Patients With Allergic Rhinitis, Particularly Concerning Rab27a ExpressionYun Liao0Minyao Li1Shuo Song2Xuejie Xu3Xiaojun Xiao4Yu Liu5Gui Yang6Pingchang Yang7Department of Otolaryngology Longgang Central Hospital Shenzhen ChinaDepartment of General Practice Medicine Third Affiliated Hospital of Shenzhen University Shenzhen ChinaDepartment of General Practice Medicine Third Affiliated Hospital of Shenzhen University Shenzhen ChinaInstitute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Allergy Division at Shenzhen University and Shenzhen Key Laboratory of Allergy & Immunology Shenzhen ChinaInstitute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Allergy Division at Shenzhen University and Shenzhen Key Laboratory of Allergy & Immunology Shenzhen ChinaDepartment of General Practice Medicine Third Affiliated Hospital of Shenzhen University Shenzhen ChinaDepartment of Otolaryngology Longgang Central Hospital Shenzhen ChinaDepartment of General Practice Medicine Third Affiliated Hospital of Shenzhen University Shenzhen ChinaABSTRACT Background Eosinophils have been acknowledged to be involved in the induction of numerous inflammatory disorders. There is still a lack of knowledge about whether eosinophils play a role in immune regulation. The aim of this study is to uncover the immune regulatory functions of eosinophils. Methods Blood samples were collected from patients with allergic rhinitis (AR) and healthy control subjects. Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples. Eosinophils were purified from PBMCs using flow cytometry cell sorting and analyzed using immunological approaches. Results The results showed that eosinophils from healthy subjects had immune regulatory functions on T cell proliferation and cytokine release. Impairment of eosinophil immune regulatory functions was found in AR patients, which was associated with AR responses. Elevated Rab27a expression in eosinophils was associated with their impaired immune regulatory functions and the increased AR responses. Rab27a controlled the release of mediators from eosinophils. Low concentrations of Eosinophil mediators could trigger immune regulatory responses, while high concentrations could trigger inflammatory responses. Regulating Rab27a restored the immune regulatory functions of eosinophils of AR patients. Conclusions Eosinophils have immune regulatory functions, which are controlled by the expression of Rab27a. Regulation of Rab27a can improve the immune regulatory functions of eosinophils. The data suggest that inhibition of Rab27a can be a drug candidate for the treatment of eosinophil‐related disorders.https://doi.org/10.1002/iid3.70091allergycytokineeosinophilimmune regulationrhinitis |
| spellingShingle | Yun Liao Minyao Li Shuo Song Xuejie Xu Xiaojun Xiao Yu Liu Gui Yang Pingchang Yang The Immunosuppressive Functions of Eosinophils Are Compromised in Patients With Allergic Rhinitis, Particularly Concerning Rab27a Expression Immunity, Inflammation and Disease allergy cytokine eosinophil immune regulation rhinitis |
| title | The Immunosuppressive Functions of Eosinophils Are Compromised in Patients With Allergic Rhinitis, Particularly Concerning Rab27a Expression |
| title_full | The Immunosuppressive Functions of Eosinophils Are Compromised in Patients With Allergic Rhinitis, Particularly Concerning Rab27a Expression |
| title_fullStr | The Immunosuppressive Functions of Eosinophils Are Compromised in Patients With Allergic Rhinitis, Particularly Concerning Rab27a Expression |
| title_full_unstemmed | The Immunosuppressive Functions of Eosinophils Are Compromised in Patients With Allergic Rhinitis, Particularly Concerning Rab27a Expression |
| title_short | The Immunosuppressive Functions of Eosinophils Are Compromised in Patients With Allergic Rhinitis, Particularly Concerning Rab27a Expression |
| title_sort | immunosuppressive functions of eosinophils are compromised in patients with allergic rhinitis particularly concerning rab27a expression |
| topic | allergy cytokine eosinophil immune regulation rhinitis |
| url | https://doi.org/10.1002/iid3.70091 |
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