PcWRKY1 Represses Transcription of Yellow Stripe‐Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar Stems
Abstract A considerable amount of cadmium (Cd) can accumulate in the bark of poplar stems, but the Cd transport pathway and its underlying molecular mechanisms remain unknown. Here, a Cd radial transport pathway in poplar stems and a previously unrecognized PcWRKY1‐Yellow Stripe‐Like 3 (PcYSL3) modu...
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Wiley
2025-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202405492 |
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| author | Xin Chen Yuhong Zhang Yao Cheng Wenjian Yu Lingyu Yang Peiqi Shu Jing Zhou Payam Fayyaz Zhi‐Bin Luo Shurong Deng Wenguang Shi |
| author_facet | Xin Chen Yuhong Zhang Yao Cheng Wenjian Yu Lingyu Yang Peiqi Shu Jing Zhou Payam Fayyaz Zhi‐Bin Luo Shurong Deng Wenguang Shi |
| author_sort | Xin Chen |
| collection | DOAJ |
| description | Abstract A considerable amount of cadmium (Cd) can accumulate in the bark of poplar stems, but the Cd transport pathway and its underlying molecular mechanisms remain unknown. Here, a Cd radial transport pathway in poplar stems and a previously unrecognized PcWRKY1‐Yellow Stripe‐Like 3 (PcYSL3) module that regulates Cd transport are identified in Populus × canescens (Aiton) Sm. Cadmiun‐nicotianamine (Cd‐NA) in xylem vessels in poplar stem‐wood is unloaded to adjacent ray parenchyma cells and further radially transported to bark‐phloem. PcYSL3 is putatively identified as involved in Cd radial transport in poplar stems. PcYSL3 is highly expressed in ray parenchyma cells adjacent to xylem vessels and the encoded protein localizes on the plasma membrane. Cd accumulation is greater in the wood and bark of PcYSL3‐overexpressing poplars than the wild type, whereas the opposite is observed in PcYSL3‐knockdown plants. PcWRKY1 can bind to the PcYSL3 promoter sequence and represses its expression. PcWRKY1 inhibits Cd accumulation in the wood and bark of plants. Thus, PcWRKY1 suppresses PcYSL3 transcription to negatively regulate Cd‐NA unloading from xylem vessels to adjacent ray parenchyma cells and its radial transport in poplar stem. The findings have provided new insights into breeding of poplars for more effective remediation of heavy metal‐contaminated soils. |
| format | Article |
| id | doaj-art-dc21c548230b4632a38d03cbb9b679f6 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-dc21c548230b4632a38d03cbb9b679f62025-01-09T11:44:45ZengWileyAdvanced Science2198-38442025-01-01121n/an/a10.1002/advs.202405492PcWRKY1 Represses Transcription of Yellow Stripe‐Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar StemsXin Chen0Yuhong Zhang1Yao Cheng2Wenjian Yu3Lingyu Yang4Peiqi Shu5Jing Zhou6Payam Fayyaz7Zhi‐Bin Luo8Shurong Deng9Wenguang Shi10State Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 P. R. ChinaState Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 P. R. ChinaState Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 P. R. ChinaState Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 P. R. ChinaState Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 P. R. ChinaState Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 P. R. ChinaState Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 P. R. ChinaForest, Range and Watershed Management Department Agriculture and Natural Resources Faculty Yasouj University Yasuj 75919 63179 IranState Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 P. R. ChinaState Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 P. R. ChinaState Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 P. R. ChinaAbstract A considerable amount of cadmium (Cd) can accumulate in the bark of poplar stems, but the Cd transport pathway and its underlying molecular mechanisms remain unknown. Here, a Cd radial transport pathway in poplar stems and a previously unrecognized PcWRKY1‐Yellow Stripe‐Like 3 (PcYSL3) module that regulates Cd transport are identified in Populus × canescens (Aiton) Sm. Cadmiun‐nicotianamine (Cd‐NA) in xylem vessels in poplar stem‐wood is unloaded to adjacent ray parenchyma cells and further radially transported to bark‐phloem. PcYSL3 is putatively identified as involved in Cd radial transport in poplar stems. PcYSL3 is highly expressed in ray parenchyma cells adjacent to xylem vessels and the encoded protein localizes on the plasma membrane. Cd accumulation is greater in the wood and bark of PcYSL3‐overexpressing poplars than the wild type, whereas the opposite is observed in PcYSL3‐knockdown plants. PcWRKY1 can bind to the PcYSL3 promoter sequence and represses its expression. PcWRKY1 inhibits Cd accumulation in the wood and bark of plants. Thus, PcWRKY1 suppresses PcYSL3 transcription to negatively regulate Cd‐NA unloading from xylem vessels to adjacent ray parenchyma cells and its radial transport in poplar stem. The findings have provided new insights into breeding of poplars for more effective remediation of heavy metal‐contaminated soils.https://doi.org/10.1002/advs.202405492heavy metalpopulustranscriptional regulationtransporterxylem‐to‐phloem transport |
| spellingShingle | Xin Chen Yuhong Zhang Yao Cheng Wenjian Yu Lingyu Yang Peiqi Shu Jing Zhou Payam Fayyaz Zhi‐Bin Luo Shurong Deng Wenguang Shi PcWRKY1 Represses Transcription of Yellow Stripe‐Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar Stems Advanced Science heavy metal populus transcriptional regulation transporter xylem‐to‐phloem transport |
| title | PcWRKY1 Represses Transcription of Yellow Stripe‐Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar Stems |
| title_full | PcWRKY1 Represses Transcription of Yellow Stripe‐Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar Stems |
| title_fullStr | PcWRKY1 Represses Transcription of Yellow Stripe‐Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar Stems |
| title_full_unstemmed | PcWRKY1 Represses Transcription of Yellow Stripe‐Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar Stems |
| title_short | PcWRKY1 Represses Transcription of Yellow Stripe‐Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar Stems |
| title_sort | pcwrky1 represses transcription of yellow stripe like 3 pcysl3 to negatively regulate radial cadmium transport in poplar stems |
| topic | heavy metal populus transcriptional regulation transporter xylem‐to‐phloem transport |
| url | https://doi.org/10.1002/advs.202405492 |
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