Icariin on the proliferation and osteogenic differentiation of MC3T3-E1: An experimental study
[Objective:] To study the effects of a Chinese traditional medicine icariin (ICA) on the cell activity and osteogenic differentiation of preosteoblasts in mice calvarium (MC3T3-E1). [Methods:] The optimal intervention concentration of ICA was screened and MC3T3-E1 cells were divided into blank contr...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-08-01
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| Series: | Kouqiang hemian waike zazhi |
| Subjects: | |
| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.04.003 |
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| Summary: | [Objective:] To study the effects of a Chinese traditional medicine icariin (ICA) on the cell activity and osteogenic differentiation of preosteoblasts in mice calvarium (MC3T3-E1). [Methods:] The optimal intervention concentration of ICA was screened and MC3T3-E1 cells were divided into blank control group and experimental group with different concentrations of ICA. MC3T3-E1 cells were treated with ICA complete medium containing 10-9, 10-8, 10-7, 10-6 and 10-5 mol/L. On days 2, 4 and 6, the proliferation of MC3T3-E1 cells was detected by CCK8 kit, and the ALP activity was detected by alkaline phosphatase (ALP) kit on days 3, 6 and 9; MC3T3-E1 cells were treated with 10-7 mol/L ICA complete medium for 21 days. Alizarin red (AR) staining was used to determine the formation of calcium nodules. [Results:] Compared with the blank control group, the drug treatment group significantly inhibited the proliferation of MC3T3-E1 cells, but promoted the ALP activity of MC3T3-E1 cells; however, different concentrations of ICA had no significant effect on the formation of calcium nodules in cultured cells. [Conclusion:] Under our experimental conditions, ICA may inhibit the proliferation of MC3T3-E1 cells and promote osteogenic differentiation of MC3T3-E1 cells. |
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| ISSN: | 1005-4979 |