Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord blood
Abstract Umbilical cord blood (UCB) units are an alternative source of human hematopoietic stem cells (HSCs) for allogeneic stem cell transplants. A large quantity of HSCs is needed but the low number of accessible cells from UCB has been a significant limitation. Improving the ex vivo growth of HSC...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-15647-9 |
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| author | Nareerat Sutjarit Laongthip Ruknarong Chamnan Tanprasertkul Kanit Bhukhai Hay Man Saung Hnin Soe Pakpoom Kheolamai Sirikul Manochantr Chairat Tantrawatpan Duangrat Tantikanlayaporn |
| author_facet | Nareerat Sutjarit Laongthip Ruknarong Chamnan Tanprasertkul Kanit Bhukhai Hay Man Saung Hnin Soe Pakpoom Kheolamai Sirikul Manochantr Chairat Tantrawatpan Duangrat Tantikanlayaporn |
| author_sort | Nareerat Sutjarit |
| collection | DOAJ |
| description | Abstract Umbilical cord blood (UCB) units are an alternative source of human hematopoietic stem cells (HSCs) for allogeneic stem cell transplants. A large quantity of HSCs is needed but the low number of accessible cells from UCB has been a significant limitation. Improving the ex vivo growth of HSCs while preserving their functioning is required. Here, we report that andrographolide (AP) enhanced the expansion of human UCB-derived HSCs (HSPCs) and pro-moted primitive HSCs (CD34+CD38−CD90+). AP also improved HSC functionality, evidenced by increased growth of colony-forming units and multilineage differentiation. AP upregulated genes involved in the Wnt/β-catenin and Notch signaling pathways. AP also modulated signaling pathways involved in HSC self-renewal, proliferation, survival, and differentiation, demonstrated by Nanostring analysis. The results of this study suggest that andrographolide enhances ex vivo UCB-HSC expansion while maintaining functionality and has potential for treatment of hematological diseases. |
| format | Article |
| id | doaj-art-db12851aad104d3287dd3734ed3d7cd9 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-db12851aad104d3287dd3734ed3d7cd92025-08-20T03:45:52ZengNature PortfolioScientific Reports2045-23222025-08-0115111310.1038/s41598-025-15647-9Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord bloodNareerat Sutjarit0Laongthip Ruknarong1Chamnan Tanprasertkul2Kanit Bhukhai3Hay Man Saung Hnin Soe4Pakpoom Kheolamai5Sirikul Manochantr6Chairat Tantrawatpan7Duangrat Tantikanlayaporn8Nutrition Unit, Faculty of Medicine Ramathibodi Hospital, Mahidol UniversityCenter of Excellence in Stem Research and Innovation, Thammasat UniversityThammasat University Research Unit in Advanced Clinical Data Statistics Analysis and Innovative Research in Obstetrics and Gynecology GroupDepartment of Physiology, Faculty of Science, Mahidol UniversityCenter of Excellence in Stem Research and Innovation, Thammasat UniversityCenter of Excellence in Stem Research and Innovation, Thammasat UniversityCenter of Excellence in Stem Research and Innovation, Thammasat UniversityCenter of Excellence in Stem Research and Innovation, Thammasat UniversityCenter of Excellence in Stem Research and Innovation, Thammasat UniversityAbstract Umbilical cord blood (UCB) units are an alternative source of human hematopoietic stem cells (HSCs) for allogeneic stem cell transplants. A large quantity of HSCs is needed but the low number of accessible cells from UCB has been a significant limitation. Improving the ex vivo growth of HSCs while preserving their functioning is required. Here, we report that andrographolide (AP) enhanced the expansion of human UCB-derived HSCs (HSPCs) and pro-moted primitive HSCs (CD34+CD38−CD90+). AP also improved HSC functionality, evidenced by increased growth of colony-forming units and multilineage differentiation. AP upregulated genes involved in the Wnt/β-catenin and Notch signaling pathways. AP also modulated signaling pathways involved in HSC self-renewal, proliferation, survival, and differentiation, demonstrated by Nanostring analysis. The results of this study suggest that andrographolide enhances ex vivo UCB-HSC expansion while maintaining functionality and has potential for treatment of hematological diseases.https://doi.org/10.1038/s41598-025-15647-9AndrographolideHematopoietic stem cells (HSCs)Ex vivo expansionUmbilical cord blood (UCB)Hematologic disordersHematopoietic stem cell transplantation |
| spellingShingle | Nareerat Sutjarit Laongthip Ruknarong Chamnan Tanprasertkul Kanit Bhukhai Hay Man Saung Hnin Soe Pakpoom Kheolamai Sirikul Manochantr Chairat Tantrawatpan Duangrat Tantikanlayaporn Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord blood Scientific Reports Andrographolide Hematopoietic stem cells (HSCs) Ex vivo expansion Umbilical cord blood (UCB) Hematologic disorders Hematopoietic stem cell transplantation |
| title | Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord blood |
| title_full | Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord blood |
| title_fullStr | Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord blood |
| title_full_unstemmed | Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord blood |
| title_short | Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord blood |
| title_sort | andrographolide promotes the ex vivo expansion of cd34 hematopoietic stem cells derived from human umbilical cord blood |
| topic | Andrographolide Hematopoietic stem cells (HSCs) Ex vivo expansion Umbilical cord blood (UCB) Hematologic disorders Hematopoietic stem cell transplantation |
| url | https://doi.org/10.1038/s41598-025-15647-9 |
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