Protective efficacy of a recombinant adenovirus expressing novel dual F and HN proteins of bovine parainfluenza virus type 3
Abstract Bovine parainfluenza virus type 3 (BPIV3) is a viral respiratory pathogen that infects cattle and causes significant economic losses. We generated a recombinant adenovirus called rHAd5-F + HN by expressing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein of BPIV3 using the h...
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| Language: | English |
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BMC
2024-11-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-024-01400-z |
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| author | Jiaqi Zhang Jinbo Wu Qing Zhu Xiangyue Huang Zhaohui Zhang Chenxi Zhu Gunan Deng Ajia Ake Yuanzhen Ma Chunsai He Rui Guo Hua Yue Lan Lan Bin Zhang |
| author_facet | Jiaqi Zhang Jinbo Wu Qing Zhu Xiangyue Huang Zhaohui Zhang Chenxi Zhu Gunan Deng Ajia Ake Yuanzhen Ma Chunsai He Rui Guo Hua Yue Lan Lan Bin Zhang |
| author_sort | Jiaqi Zhang |
| collection | DOAJ |
| description | Abstract Bovine parainfluenza virus type 3 (BPIV3) is a viral respiratory pathogen that infects cattle and causes significant economic losses. We generated a recombinant adenovirus called rHAd5-F + HN by expressing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein of BPIV3 using the human adenovirus serotype 5 (rHAd5). We evaluated its effects on humoral and cellular immune responses in mice (n = 45) and calves (n = 9). Serum antibody responses were assessed by enzyme-linked immunosorbent assay (ELISA), hemagglutination inhibition (HI), and neutralising antibodies (NAb). After boosting immunity with rHAd5-F + HN, mice produced significantly higher levels of antibodies against the BPIV3 genotype A and genotype C strains. The production of antibodies exceeded those produced by adenoviruses rHAd5-F and rHAd5-HN, which express the F and HN glycoprotein, respectively. The percentages of splenic CD3+/CD8+T lymphocytes and IL-4+ cytokines in rHAd5-F + HN mice were considerably higher than those in the control group. Mice immunised with rHAd5-F + HN exhibited much lower viral loads in the lungs and tracheas compared to the control group. Additionally, the lungs of mice vaccinated with rHAd5-F + HN showed no notable histopathological changes. On the other hand, rHAd5-F + HN produced a humoral immune response in calves. Following the booster intramuscular injection with the rHAd5-F + HN, the serum antibody levels against BPIV3 genotype C strain were 1:20 452, 1:1024, and 1:426 in calves, as detected by ELISA, HI, and NAb, respectively. The HI and NAb levels against the BPIV3 genotype A strain were 1:213 and 1:85 in calves, respectively. These results indicate that rHAd5-F + HN effectively induced immunity against BPIV3 infection. |
| format | Article |
| id | doaj-art-dab09fd5146145d881487901c43e5e0f |
| institution | Kabale University |
| issn | 1297-9716 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj-art-dab09fd5146145d881487901c43e5e0f2024-11-10T12:38:53ZengBMCVeterinary Research1297-97162024-11-0155111210.1186/s13567-024-01400-zProtective efficacy of a recombinant adenovirus expressing novel dual F and HN proteins of bovine parainfluenza virus type 3Jiaqi Zhang0Jinbo Wu1Qing Zhu2Xiangyue Huang3Zhaohui Zhang4Chenxi Zhu5Gunan Deng6Ajia Ake7Yuanzhen Ma8Chunsai He9Rui Guo10Hua Yue11Lan Lan12Bin Zhang13College of Animal and Veterinary Sciences, Southwest Minzu UniversityAnimal Husbandry Science Institute of ABa Autonomous PrefectureCenter for Animal Disease Control and Prevention, Ganzi Tibetan Autonomous PrefectueAnimal Husbandry Science Institute of ABa Autonomous PrefectureCenter for Animal Disease Control and Prevention, Ganzi Tibetan Autonomous PrefectueCollege of Animal and Veterinary Sciences, Southwest Minzu UniversityCollege of Animal and Veterinary Sciences, Southwest Minzu UniversityCollege of Animal and Veterinary Sciences, Southwest Minzu UniversityCollege of Animal and Veterinary Sciences, Southwest Minzu UniversityCollege of Animal and Veterinary Sciences, Southwest Minzu UniversityCollege of Animal and Veterinary Sciences, Southwest Minzu UniversityCollege of Animal and Veterinary Sciences, Southwest Minzu UniversityAnimal Husbandry Science Institute of Ganzi Tibetan Autonomous PrefectureCollege of Animal and Veterinary Sciences, Southwest Minzu UniversityAbstract Bovine parainfluenza virus type 3 (BPIV3) is a viral respiratory pathogen that infects cattle and causes significant economic losses. We generated a recombinant adenovirus called rHAd5-F + HN by expressing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein of BPIV3 using the human adenovirus serotype 5 (rHAd5). We evaluated its effects on humoral and cellular immune responses in mice (n = 45) and calves (n = 9). Serum antibody responses were assessed by enzyme-linked immunosorbent assay (ELISA), hemagglutination inhibition (HI), and neutralising antibodies (NAb). After boosting immunity with rHAd5-F + HN, mice produced significantly higher levels of antibodies against the BPIV3 genotype A and genotype C strains. The production of antibodies exceeded those produced by adenoviruses rHAd5-F and rHAd5-HN, which express the F and HN glycoprotein, respectively. The percentages of splenic CD3+/CD8+T lymphocytes and IL-4+ cytokines in rHAd5-F + HN mice were considerably higher than those in the control group. Mice immunised with rHAd5-F + HN exhibited much lower viral loads in the lungs and tracheas compared to the control group. Additionally, the lungs of mice vaccinated with rHAd5-F + HN showed no notable histopathological changes. On the other hand, rHAd5-F + HN produced a humoral immune response in calves. Following the booster intramuscular injection with the rHAd5-F + HN, the serum antibody levels against BPIV3 genotype C strain were 1:20 452, 1:1024, and 1:426 in calves, as detected by ELISA, HI, and NAb, respectively. The HI and NAb levels against the BPIV3 genotype A strain were 1:213 and 1:85 in calves, respectively. These results indicate that rHAd5-F + HN effectively induced immunity against BPIV3 infection.https://doi.org/10.1186/s13567-024-01400-zBovine parainfluenza virus type 3adenovirus vector vaccineF and HN proteinsprotection efficacy |
| spellingShingle | Jiaqi Zhang Jinbo Wu Qing Zhu Xiangyue Huang Zhaohui Zhang Chenxi Zhu Gunan Deng Ajia Ake Yuanzhen Ma Chunsai He Rui Guo Hua Yue Lan Lan Bin Zhang Protective efficacy of a recombinant adenovirus expressing novel dual F and HN proteins of bovine parainfluenza virus type 3 Veterinary Research Bovine parainfluenza virus type 3 adenovirus vector vaccine F and HN proteins protection efficacy |
| title | Protective efficacy of a recombinant adenovirus expressing novel dual F and HN proteins of bovine parainfluenza virus type 3 |
| title_full | Protective efficacy of a recombinant adenovirus expressing novel dual F and HN proteins of bovine parainfluenza virus type 3 |
| title_fullStr | Protective efficacy of a recombinant adenovirus expressing novel dual F and HN proteins of bovine parainfluenza virus type 3 |
| title_full_unstemmed | Protective efficacy of a recombinant adenovirus expressing novel dual F and HN proteins of bovine parainfluenza virus type 3 |
| title_short | Protective efficacy of a recombinant adenovirus expressing novel dual F and HN proteins of bovine parainfluenza virus type 3 |
| title_sort | protective efficacy of a recombinant adenovirus expressing novel dual f and hn proteins of bovine parainfluenza virus type 3 |
| topic | Bovine parainfluenza virus type 3 adenovirus vector vaccine F and HN proteins protection efficacy |
| url | https://doi.org/10.1186/s13567-024-01400-z |
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