Development of a full field-of-view spectroscopic single-molecule fluorescence instrument with improved methods for calibration
We have developed a high-throughput, full field-of-view (FOV) spectroscopic single-molecule fluorescence instrument, overcoming distortion issues with gratings and prisms. Our calibration techniques ensure spectral uniformity across the whole FOV, and our two-step Gaussian fit method enables better...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
IOP Publishing
2025-01-01
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| Series: | Applied Physics Express |
| Subjects: | |
| Online Access: | https://doi.org/10.35848/1882-0786/ada10b |
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